A Wearable and Deformable Graphene-Based Affinity Nanosensor for Monitoring of Cytokines in Biofluids
A wearable and deformable graphene-based field-effect transistor biosensor is presented that uses aptamer-modified graphene as the conducting channel, which is capable of the sensitive, consistent and time-resolved detection of cytokines in human biofluids. Based on an ultrathin substrate, the biose...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-07-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/10/8/1503 |
| _version_ | 1827711891682623488 |
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| author | Ziran Wang Zhuang Hao Shifeng Yu Cong Huang Yunlu Pan Xuezeng Zhao |
| author_facet | Ziran Wang Zhuang Hao Shifeng Yu Cong Huang Yunlu Pan Xuezeng Zhao |
| author_sort | Ziran Wang |
| collection | DOAJ |
| description | A wearable and deformable graphene-based field-effect transistor biosensor is presented that uses aptamer-modified graphene as the conducting channel, which is capable of the sensitive, consistent and time-resolved detection of cytokines in human biofluids. Based on an ultrathin substrate, the biosensor offers a high level of mechanical durability and consistent sensing responses, while conforming to non-planar surfaces such as the human body and withstanding large deformations (e.g., bending and stretching). Moreover, a nonionic surfactant is employed to minimize the nonspecific adsorption of the biosensor, hence enabling cytokine detection (TNF-α and IFN-γ, significant inflammatory cytokines, are used as representatives) in artificial tears (used as a biofluid representative). The experimental results demonstrate that the biosensor very consistently and sensitively detects TNF-α and IFN-γ, with limits of detection down to 2.75 and 2.89 pM, respectively. The biosensor, which undergoes large deformations, can thus potentially provide a consistent and sensitive detection of cytokines in the human body. |
| first_indexed | 2024-03-10T18:03:53Z |
| format | Article |
| id | doaj.art-26bbf49e682b4648ad72aa0dd9018bfa |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T18:03:53Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-26bbf49e682b4648ad72aa0dd9018bfa2023-11-20T08:36:15ZengMDPI AGNanomaterials2079-49912020-07-01108150310.3390/nano10081503A Wearable and Deformable Graphene-Based Affinity Nanosensor for Monitoring of Cytokines in BiofluidsZiran Wang0Zhuang Hao1Shifeng Yu2Cong Huang3Yunlu Pan4Xuezeng Zhao5Key Laboratory of Micro-Systems and Micro-Structures Manufacturing, Harbin Institute of Technology, Ministry of Education, Harbin 150080, ChinaKey Laboratory of Micro-Systems and Micro-Structures Manufacturing, Harbin Institute of Technology, Ministry of Education, Harbin 150080, ChinaDepartment of Mechanical Engineering, Columbia University, New York, NY 10027, USAKey Laboratory of Micro-Systems and Micro-Structures Manufacturing, Harbin Institute of Technology, Ministry of Education, Harbin 150080, ChinaKey Laboratory of Micro-Systems and Micro-Structures Manufacturing, Harbin Institute of Technology, Ministry of Education, Harbin 150080, ChinaKey Laboratory of Micro-Systems and Micro-Structures Manufacturing, Harbin Institute of Technology, Ministry of Education, Harbin 150080, ChinaA wearable and deformable graphene-based field-effect transistor biosensor is presented that uses aptamer-modified graphene as the conducting channel, which is capable of the sensitive, consistent and time-resolved detection of cytokines in human biofluids. Based on an ultrathin substrate, the biosensor offers a high level of mechanical durability and consistent sensing responses, while conforming to non-planar surfaces such as the human body and withstanding large deformations (e.g., bending and stretching). Moreover, a nonionic surfactant is employed to minimize the nonspecific adsorption of the biosensor, hence enabling cytokine detection (TNF-α and IFN-γ, significant inflammatory cytokines, are used as representatives) in artificial tears (used as a biofluid representative). The experimental results demonstrate that the biosensor very consistently and sensitively detects TNF-α and IFN-γ, with limits of detection down to 2.75 and 2.89 pM, respectively. The biosensor, which undergoes large deformations, can thus potentially provide a consistent and sensitive detection of cytokines in the human body.https://www.mdpi.com/2079-4991/10/8/1503wearable sensorgraphene field-effect transistorcytokineultrathin sensor |
| spellingShingle | Ziran Wang Zhuang Hao Shifeng Yu Cong Huang Yunlu Pan Xuezeng Zhao A Wearable and Deformable Graphene-Based Affinity Nanosensor for Monitoring of Cytokines in Biofluids Nanomaterials wearable sensor graphene field-effect transistor cytokine ultrathin sensor |
| title | A Wearable and Deformable Graphene-Based Affinity Nanosensor for Monitoring of Cytokines in Biofluids |
| title_full | A Wearable and Deformable Graphene-Based Affinity Nanosensor for Monitoring of Cytokines in Biofluids |
| title_fullStr | A Wearable and Deformable Graphene-Based Affinity Nanosensor for Monitoring of Cytokines in Biofluids |
| title_full_unstemmed | A Wearable and Deformable Graphene-Based Affinity Nanosensor for Monitoring of Cytokines in Biofluids |
| title_short | A Wearable and Deformable Graphene-Based Affinity Nanosensor for Monitoring of Cytokines in Biofluids |
| title_sort | wearable and deformable graphene based affinity nanosensor for monitoring of cytokines in biofluids |
| topic | wearable sensor graphene field-effect transistor cytokine ultrathin sensor |
| url | https://www.mdpi.com/2079-4991/10/8/1503 |
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