Effect of vitamin A supplementation at different gaseous environments on in vitro development of pre-implantation sheep embryos to the blastocyst stage
Vitamin A (all-trans retinol) is an important antioxidant whose role in embryo development in vitro and in vivo is well established. Oxidative stress is a major cause of defective embryo development. This study evaluated the effects of all-trans retinol supplementation to maturation and embryo cultu...
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| Format: | Article |
| Language: | English |
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Elsevier
2010-01-01
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| Series: | Animal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1751731110001187 |
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| author | N. Rajesh M.B. Shankar M. Deecaraman |
| author_facet | N. Rajesh M.B. Shankar M. Deecaraman |
| author_sort | N. Rajesh |
| collection | DOAJ |
| description | Vitamin A (all-trans retinol) is an important antioxidant whose role in embryo development in vitro and in vivo is well established. Oxidative stress is a major cause of defective embryo development. This study evaluated the effects of all-trans retinol supplementation to maturation and embryo culture media under different gaseous environments on the development of ovine oocytes and embryos in vitro. The percentages of cleavage, morula and blastocyst, total cell count and comet assay were taken as indicators of developmental competence of embryos. In experiments I and II, all-trans retinol at concentrations of 0, 2, 4, 6, 8 and 10 μM were supplemented to the oocyte maturation medium and cultured in an environment of 5% or 20% O2 respectively. All-trans retinol supplementation (6 μM) to the maturation medium at 5% O2 levels significantly increased blastocyst yield and total cell number (P < 0.05). Maturation of oocytes in a 20% O2 environment bettered cleavage rates in the 6 μM supplemented group compared with the control group (P < 0.05). In experiments III and IV, all-trans retinol, at the aforesaid concentrations was supplemented to embryo culture media under a 5% or 20% O2 environment, respectively. All-trans retinol supplementation to the embryo culture medium at 5% O2 levels did not yield any significant result whereas the culture at 20% O2 levels gave significantly higher blastocyst yield in the 6 μM supplemented group compared with the control group (P < 0.01). |
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| format | Article |
| id | doaj.art-26be642515f04c0791134b614fae5a37 |
| institution | Directory Open Access Journal |
| issn | 1751-7311 |
| language | English |
| last_indexed | 2024-12-19T03:21:25Z |
| publishDate | 2010-01-01 |
| publisher | Elsevier |
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| series | Animal |
| spelling | doaj.art-26be642515f04c0791134b614fae5a372022-12-21T20:37:45ZengElsevierAnimal1751-73112010-01-0141118841890Effect of vitamin A supplementation at different gaseous environments on in vitro development of pre-implantation sheep embryos to the blastocyst stageN. Rajesh0M.B. Shankar1M. Deecaraman2Dr MGR Educational and Research Institute, Dr MGR University, Maduravoyal, Chennai – 600095, Tamilnadu, IndiaQuality Assurance Department, Huclin Research Limited, TICEL Biopark, Taramani, Chennai-600113, Tamilnadu, IndiaDr MGR Educational and Research Institute, Dr MGR University, Maduravoyal, Chennai – 600095, Tamilnadu, IndiaVitamin A (all-trans retinol) is an important antioxidant whose role in embryo development in vitro and in vivo is well established. Oxidative stress is a major cause of defective embryo development. This study evaluated the effects of all-trans retinol supplementation to maturation and embryo culture media under different gaseous environments on the development of ovine oocytes and embryos in vitro. The percentages of cleavage, morula and blastocyst, total cell count and comet assay were taken as indicators of developmental competence of embryos. In experiments I and II, all-trans retinol at concentrations of 0, 2, 4, 6, 8 and 10 μM were supplemented to the oocyte maturation medium and cultured in an environment of 5% or 20% O2 respectively. All-trans retinol supplementation (6 μM) to the maturation medium at 5% O2 levels significantly increased blastocyst yield and total cell number (P < 0.05). Maturation of oocytes in a 20% O2 environment bettered cleavage rates in the 6 μM supplemented group compared with the control group (P < 0.05). In experiments III and IV, all-trans retinol, at the aforesaid concentrations was supplemented to embryo culture media under a 5% or 20% O2 environment, respectively. All-trans retinol supplementation to the embryo culture medium at 5% O2 levels did not yield any significant result whereas the culture at 20% O2 levels gave significantly higher blastocyst yield in the 6 μM supplemented group compared with the control group (P < 0.01).http://www.sciencedirect.com/science/article/pii/S1751731110001187vitamin Aovinein vitro fertilisationoxidative stressembryo culture |
| spellingShingle | N. Rajesh M.B. Shankar M. Deecaraman Effect of vitamin A supplementation at different gaseous environments on in vitro development of pre-implantation sheep embryos to the blastocyst stage Animal vitamin A ovine in vitro fertilisation oxidative stress embryo culture |
| title | Effect of vitamin A supplementation at different gaseous environments on in vitro development of pre-implantation sheep embryos to the blastocyst stage |
| title_full | Effect of vitamin A supplementation at different gaseous environments on in vitro development of pre-implantation sheep embryos to the blastocyst stage |
| title_fullStr | Effect of vitamin A supplementation at different gaseous environments on in vitro development of pre-implantation sheep embryos to the blastocyst stage |
| title_full_unstemmed | Effect of vitamin A supplementation at different gaseous environments on in vitro development of pre-implantation sheep embryos to the blastocyst stage |
| title_short | Effect of vitamin A supplementation at different gaseous environments on in vitro development of pre-implantation sheep embryos to the blastocyst stage |
| title_sort | effect of vitamin a supplementation at different gaseous environments on in vitro development of pre implantation sheep embryos to the blastocyst stage |
| topic | vitamin A ovine in vitro fertilisation oxidative stress embryo culture |
| url | http://www.sciencedirect.com/science/article/pii/S1751731110001187 |
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