Resveratrol Alleviates the Early Challenges of Implant-Based Drug Delivery in a Human Glial Cell Model
Brain diseases are oftentimes life-threatening and difficult to treat. The local administration of drug substances using brain implants can increase on-site concentrations and decrease systemic side effects. However, the biocompatibility of potential brain implant materials needs to be evaluated car...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/4/2078 |
| _version_ | 1797297991603716096 |
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| author | Luise Schlotterose François Cossais Ralph Lucius Kirsten Hattermann |
| author_facet | Luise Schlotterose François Cossais Ralph Lucius Kirsten Hattermann |
| author_sort | Luise Schlotterose |
| collection | DOAJ |
| description | Brain diseases are oftentimes life-threatening and difficult to treat. The local administration of drug substances using brain implants can increase on-site concentrations and decrease systemic side effects. However, the biocompatibility of potential brain implant materials needs to be evaluated carefully as implants can trigger foreign body reactions, particularly by increasing the microglia and astrocyte reactivity. To date, these tests have been frequently conducted in very simple in vitro models, in particular not respecting the key players in glial cell reactions and the challenges of surgical implantation characterized by the disruption of oxygen and nutrient supply. Thus, we established an in vitro model in which we treated human glial cell lines with reduced oxygen and glucose levels. The model displayed cytokine and reactive oxygen species release from reactive microglia and an increase in a marker of reactive astrocytes, galectin-3. Moreover, the treatment caused changes in the cell survival and triggered the production of hypoxia-inducible factor 1α. In this comprehensive platform, we demonstrated the protective effect of the natural polyphenol resveratrol as a model substance, which might be included in brain implants to ease the undesired glial cell response. Overall, a glial-cell-based in vitro model of the initial challenges of local brain disease treatment may prove useful for investigating new therapy options. |
| first_indexed | 2024-03-07T22:29:26Z |
| format | Article |
| id | doaj.art-26c05867cc8246e4b35065d854a4d313 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-07T22:29:26Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-26c05867cc8246e4b35065d854a4d3132024-02-23T15:19:40ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-02-01254207810.3390/ijms25042078Resveratrol Alleviates the Early Challenges of Implant-Based Drug Delivery in a Human Glial Cell ModelLuise Schlotterose0François Cossais1Ralph Lucius2Kirsten Hattermann3Institute of Anatomy, Kiel University, 24118 Kiel, GermanyInstitute of Anatomy, Kiel University, 24118 Kiel, GermanyInstitute of Anatomy, Kiel University, 24118 Kiel, GermanyInstitute of Anatomy, Kiel University, 24118 Kiel, GermanyBrain diseases are oftentimes life-threatening and difficult to treat. The local administration of drug substances using brain implants can increase on-site concentrations and decrease systemic side effects. However, the biocompatibility of potential brain implant materials needs to be evaluated carefully as implants can trigger foreign body reactions, particularly by increasing the microglia and astrocyte reactivity. To date, these tests have been frequently conducted in very simple in vitro models, in particular not respecting the key players in glial cell reactions and the challenges of surgical implantation characterized by the disruption of oxygen and nutrient supply. Thus, we established an in vitro model in which we treated human glial cell lines with reduced oxygen and glucose levels. The model displayed cytokine and reactive oxygen species release from reactive microglia and an increase in a marker of reactive astrocytes, galectin-3. Moreover, the treatment caused changes in the cell survival and triggered the production of hypoxia-inducible factor 1α. In this comprehensive platform, we demonstrated the protective effect of the natural polyphenol resveratrol as a model substance, which might be included in brain implants to ease the undesired glial cell response. Overall, a glial-cell-based in vitro model of the initial challenges of local brain disease treatment may prove useful for investigating new therapy options.https://www.mdpi.com/1422-0067/25/4/2078brain implantsforeign body reactionoxygen deprivationglucose deprivationreactive astrogliosisneuroinflammation |
| spellingShingle | Luise Schlotterose François Cossais Ralph Lucius Kirsten Hattermann Resveratrol Alleviates the Early Challenges of Implant-Based Drug Delivery in a Human Glial Cell Model International Journal of Molecular Sciences brain implants foreign body reaction oxygen deprivation glucose deprivation reactive astrogliosis neuroinflammation |
| title | Resveratrol Alleviates the Early Challenges of Implant-Based Drug Delivery in a Human Glial Cell Model |
| title_full | Resveratrol Alleviates the Early Challenges of Implant-Based Drug Delivery in a Human Glial Cell Model |
| title_fullStr | Resveratrol Alleviates the Early Challenges of Implant-Based Drug Delivery in a Human Glial Cell Model |
| title_full_unstemmed | Resveratrol Alleviates the Early Challenges of Implant-Based Drug Delivery in a Human Glial Cell Model |
| title_short | Resveratrol Alleviates the Early Challenges of Implant-Based Drug Delivery in a Human Glial Cell Model |
| title_sort | resveratrol alleviates the early challenges of implant based drug delivery in a human glial cell model |
| topic | brain implants foreign body reaction oxygen deprivation glucose deprivation reactive astrogliosis neuroinflammation |
| url | https://www.mdpi.com/1422-0067/25/4/2078 |
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