Evaluation of Biofilm Formation in <i>Candida tropicalis</i> Using a Silicone-Based Platform with Synthetic Urine Medium
Molecular mechanisms of biofilm formation in <i>Candida tropicalis</i> and current methods for biofilm analyses in this fungal pathogen are limited. (2) Methods: Biofilm biomass and crystal violet staining of the wild-type and each gene mutant strain of <i>C. tropicalis</i> w...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-05-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/8/5/660 |
| _version_ | 1797569103094874112 |
|---|---|
| author | Yi-Kai Tseng Yu-Chia Chen Chien-Jui Hou Fu-Sheng Deng Shen-Huan Liang Sin Yong Hoo Chih-Chieh Hsu Cai-Ling Ke Ching-Hsuan Lin |
| author_facet | Yi-Kai Tseng Yu-Chia Chen Chien-Jui Hou Fu-Sheng Deng Shen-Huan Liang Sin Yong Hoo Chih-Chieh Hsu Cai-Ling Ke Ching-Hsuan Lin |
| author_sort | Yi-Kai Tseng |
| collection | DOAJ |
| description | Molecular mechanisms of biofilm formation in <i>Candida tropicalis</i> and current methods for biofilm analyses in this fungal pathogen are limited. (2) Methods: Biofilm biomass and crystal violet staining of the wild-type and each gene mutant strain of <i>C. tropicalis</i> were evaluated on silicone under synthetic urine culture conditions. (3) Results: Seven media were tested to compare the effects on biofilm growth with or without silicone. Results showed that biofilm cells of <i>C. tropicalis</i> were unable to form firm biofilms on the bottom of 12-well polystyrene plates. However, on a silicone-based platform, Roswell Park Memorial Institute 1640 (RPMI 1640), yeast nitrogen base (YNB) + 1% glucose, and synthetic urine media were able to induce strong biofilm growth. In particular, replacement of Spider medium with synthetic urine in the adherence step and the developmental stage is necessary to gain remarkably increased biofilms. Interestingly, unlike <i>Candida albicans</i>, the <i>C. tropicalis</i> <i>ROB1</i> deletion strain but not the other five biofilm-associated mutants did not cause a significant reduction in biofilm formation, suggesting that the biofilm regulatory circuits of the two species are divergent. (4) Conclusions: This system for <i>C. tropicalis</i> biofilm analyses will become a useful tool to unveil the biofilm regulatory network in <i>C. tropicalis</i>. |
| first_indexed | 2024-03-10T20:06:15Z |
| format | Article |
| id | doaj.art-26c05df5c4f34c9b9bc3afc545076f15 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T20:06:15Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-26c05df5c4f34c9b9bc3afc545076f152023-11-19T23:13:17ZengMDPI AGMicroorganisms2076-26072020-05-018566010.3390/microorganisms8050660Evaluation of Biofilm Formation in <i>Candida tropicalis</i> Using a Silicone-Based Platform with Synthetic Urine MediumYi-Kai Tseng0Yu-Chia Chen1Chien-Jui Hou2Fu-Sheng Deng3Shen-Huan Liang4Sin Yong Hoo5Chih-Chieh Hsu6Cai-Ling Ke7Ching-Hsuan Lin8Department of Biochemical Science and Technology, College of Life Science, National Taiwan University, Taipei City 100-116, TaiwanDepartment of Biochemical Science and Technology, College of Life Science, National Taiwan University, Taipei City 100-116, TaiwanDepartment of Biochemical Science and Technology, College of Life Science, National Taiwan University, Taipei City 100-116, TaiwanDepartment of Biochemical Science and Technology, College of Life Science, National Taiwan University, Taipei City 100-116, TaiwanDepartment of Biochemical Science and Technology, College of Life Science, National Taiwan University, Taipei City 100-116, TaiwanDepartment of Biochemical Science and Technology, College of Life Science, National Taiwan University, Taipei City 100-116, TaiwanDepartment of Biochemical Science and Technology, College of Life Science, National Taiwan University, Taipei City 100-116, TaiwanDepartment of Biochemical Science and Technology, College of Life Science, National Taiwan University, Taipei City 100-116, TaiwanDepartment of Biochemical Science and Technology, College of Life Science, National Taiwan University, Taipei City 100-116, TaiwanMolecular mechanisms of biofilm formation in <i>Candida tropicalis</i> and current methods for biofilm analyses in this fungal pathogen are limited. (2) Methods: Biofilm biomass and crystal violet staining of the wild-type and each gene mutant strain of <i>C. tropicalis</i> were evaluated on silicone under synthetic urine culture conditions. (3) Results: Seven media were tested to compare the effects on biofilm growth with or without silicone. Results showed that biofilm cells of <i>C. tropicalis</i> were unable to form firm biofilms on the bottom of 12-well polystyrene plates. However, on a silicone-based platform, Roswell Park Memorial Institute 1640 (RPMI 1640), yeast nitrogen base (YNB) + 1% glucose, and synthetic urine media were able to induce strong biofilm growth. In particular, replacement of Spider medium with synthetic urine in the adherence step and the developmental stage is necessary to gain remarkably increased biofilms. Interestingly, unlike <i>Candida albicans</i>, the <i>C. tropicalis</i> <i>ROB1</i> deletion strain but not the other five biofilm-associated mutants did not cause a significant reduction in biofilm formation, suggesting that the biofilm regulatory circuits of the two species are divergent. (4) Conclusions: This system for <i>C. tropicalis</i> biofilm analyses will become a useful tool to unveil the biofilm regulatory network in <i>C. tropicalis</i>.https://www.mdpi.com/2076-2607/8/5/660<i>Candida tropicalis</i>biofilmssynthetic urine<i>ROB1</i> |
| spellingShingle | Yi-Kai Tseng Yu-Chia Chen Chien-Jui Hou Fu-Sheng Deng Shen-Huan Liang Sin Yong Hoo Chih-Chieh Hsu Cai-Ling Ke Ching-Hsuan Lin Evaluation of Biofilm Formation in <i>Candida tropicalis</i> Using a Silicone-Based Platform with Synthetic Urine Medium Microorganisms <i>Candida tropicalis</i> biofilms synthetic urine <i>ROB1</i> |
| title | Evaluation of Biofilm Formation in <i>Candida tropicalis</i> Using a Silicone-Based Platform with Synthetic Urine Medium |
| title_full | Evaluation of Biofilm Formation in <i>Candida tropicalis</i> Using a Silicone-Based Platform with Synthetic Urine Medium |
| title_fullStr | Evaluation of Biofilm Formation in <i>Candida tropicalis</i> Using a Silicone-Based Platform with Synthetic Urine Medium |
| title_full_unstemmed | Evaluation of Biofilm Formation in <i>Candida tropicalis</i> Using a Silicone-Based Platform with Synthetic Urine Medium |
| title_short | Evaluation of Biofilm Formation in <i>Candida tropicalis</i> Using a Silicone-Based Platform with Synthetic Urine Medium |
| title_sort | evaluation of biofilm formation in i candida tropicalis i using a silicone based platform with synthetic urine medium |
| topic | <i>Candida tropicalis</i> biofilms synthetic urine <i>ROB1</i> |
| url | https://www.mdpi.com/2076-2607/8/5/660 |
| work_keys_str_mv | AT yikaitseng evaluationofbiofilmformationinicandidatropicalisiusingasiliconebasedplatformwithsyntheticurinemedium AT yuchiachen evaluationofbiofilmformationinicandidatropicalisiusingasiliconebasedplatformwithsyntheticurinemedium AT chienjuihou evaluationofbiofilmformationinicandidatropicalisiusingasiliconebasedplatformwithsyntheticurinemedium AT fushengdeng evaluationofbiofilmformationinicandidatropicalisiusingasiliconebasedplatformwithsyntheticurinemedium AT shenhuanliang evaluationofbiofilmformationinicandidatropicalisiusingasiliconebasedplatformwithsyntheticurinemedium AT sinyonghoo evaluationofbiofilmformationinicandidatropicalisiusingasiliconebasedplatformwithsyntheticurinemedium AT chihchiehhsu evaluationofbiofilmformationinicandidatropicalisiusingasiliconebasedplatformwithsyntheticurinemedium AT cailingke evaluationofbiofilmformationinicandidatropicalisiusingasiliconebasedplatformwithsyntheticurinemedium AT chinghsuanlin evaluationofbiofilmformationinicandidatropicalisiusingasiliconebasedplatformwithsyntheticurinemedium |