Phylogenetic analysis, biofilm production, and antimicrobial resistance profile of Escherichia coli isolated from slaughtered pigs
ABSTRACT Enteric diseases of bacterial origin are frequent in the pig industry, of particular notoriety are the colibacillosis that mainly affect piglets and cause great damage to the swine industry worldwide. The aim of the study was to analyze phylogenetics, to detect biofilm production, and to de...
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| Format: | Article |
| Language: | English |
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Universidade Federal de Minas Gerais
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| Series: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000401309&lng=en&tlng=en |
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| author | A.S. Santos D.C.V. Lima E.F.T.S. Fernandes P.P.F. Albuquerque G.V. Gouveia M.C.A. Sá M.M. Costa J.W. Pinheiro Júnior R.A. Mota |
| author_facet | A.S. Santos D.C.V. Lima E.F.T.S. Fernandes P.P.F. Albuquerque G.V. Gouveia M.C.A. Sá M.M. Costa J.W. Pinheiro Júnior R.A. Mota |
| author_sort | A.S. Santos |
| collection | DOAJ |
| description | ABSTRACT Enteric diseases of bacterial origin are frequent in the pig industry, of particular notoriety are the colibacillosis that mainly affect piglets and cause great damage to the swine industry worldwide. The aim of the study was to analyze phylogenetics, to detect biofilm production, and to determine antimicrobial resistance profile in 126 strains of Escherichia coli isolated from swabs obtained from fragments of the small intestines of 235 healthy pigs killed in slaughterhouses in Pernambuco (Brazil) using polymerase chain reaction (PCR), adherence to microplates test and disc diffusion technique. Of the analyzed samples, 88.10% (111/126) were classified in phylogenetic group B1; 4.76% (6/126) in group D; 3.97% (5/126) in group B2 and, 3.17% (4/126) in group A. Antimicrobial resistance rates observed were: lincomycin 100% (126/126), erythromycin 100% (126/126), chlortetracycline 94.44% (119/126), cephalothin 51.59% (65/126), ampicillin 38.89% (49/126), sulfamethoxazole + trimethoprim 37.3% (47/126), ciprofloxacin 19.84% (25/126), norfloxacin 14.29% (18/126), gentamicin 8.73% (11/126) and, chloramphenicol 5.55% (7/126). Multiple antibiotic resistance (MAR) ranged from 0.2 to 0.9. Of the strains tested 46.03% (58/126) produced biofilm, and 99.21% (125/126) of the strains exhibited multi-resistance. Further studies are required to elucidate the importance of each phylogenetic group in pigs and to prevent the propagation of multi-resistant E. coli strains. |
| first_indexed | 2024-12-12T20:00:00Z |
| format | Article |
| id | doaj.art-26c2796e2d5e4ce5a665a0b2ec0e50ea |
| institution | Directory Open Access Journal |
| issn | 1678-4162 |
| language | English |
| last_indexed | 2024-12-12T20:00:00Z |
| publisher | Universidade Federal de Minas Gerais |
| record_format | Article |
| series | Arquivo Brasileiro de Medicina Veterinária e Zootecnia |
| spelling | doaj.art-26c2796e2d5e4ce5a665a0b2ec0e50ea2022-12-22T00:13:46ZengUniversidade Federal de Minas GeraisArquivo Brasileiro de Medicina Veterinária e Zootecnia1678-41627041309131510.1590/1678-4162-9926S0102-09352018000401309Phylogenetic analysis, biofilm production, and antimicrobial resistance profile of Escherichia coli isolated from slaughtered pigsA.S. SantosD.C.V. LimaE.F.T.S. FernandesP.P.F. AlbuquerqueG.V. GouveiaM.C.A. SáM.M. CostaJ.W. Pinheiro JúniorR.A. MotaABSTRACT Enteric diseases of bacterial origin are frequent in the pig industry, of particular notoriety are the colibacillosis that mainly affect piglets and cause great damage to the swine industry worldwide. The aim of the study was to analyze phylogenetics, to detect biofilm production, and to determine antimicrobial resistance profile in 126 strains of Escherichia coli isolated from swabs obtained from fragments of the small intestines of 235 healthy pigs killed in slaughterhouses in Pernambuco (Brazil) using polymerase chain reaction (PCR), adherence to microplates test and disc diffusion technique. Of the analyzed samples, 88.10% (111/126) were classified in phylogenetic group B1; 4.76% (6/126) in group D; 3.97% (5/126) in group B2 and, 3.17% (4/126) in group A. Antimicrobial resistance rates observed were: lincomycin 100% (126/126), erythromycin 100% (126/126), chlortetracycline 94.44% (119/126), cephalothin 51.59% (65/126), ampicillin 38.89% (49/126), sulfamethoxazole + trimethoprim 37.3% (47/126), ciprofloxacin 19.84% (25/126), norfloxacin 14.29% (18/126), gentamicin 8.73% (11/126) and, chloramphenicol 5.55% (7/126). Multiple antibiotic resistance (MAR) ranged from 0.2 to 0.9. Of the strains tested 46.03% (58/126) produced biofilm, and 99.21% (125/126) of the strains exhibited multi-resistance. Further studies are required to elucidate the importance of each phylogenetic group in pigs and to prevent the propagation of multi-resistant E. coli strains.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000401309&lng=en&tlng=enindústria suínacolibacilosefilogeniaantibiogramamultirresistência |
| spellingShingle | A.S. Santos D.C.V. Lima E.F.T.S. Fernandes P.P.F. Albuquerque G.V. Gouveia M.C.A. Sá M.M. Costa J.W. Pinheiro Júnior R.A. Mota Phylogenetic analysis, biofilm production, and antimicrobial resistance profile of Escherichia coli isolated from slaughtered pigs Arquivo Brasileiro de Medicina Veterinária e Zootecnia indústria suína colibacilose filogenia antibiograma multirresistência |
| title | Phylogenetic analysis, biofilm production, and antimicrobial resistance profile of Escherichia coli isolated from slaughtered pigs |
| title_full | Phylogenetic analysis, biofilm production, and antimicrobial resistance profile of Escherichia coli isolated from slaughtered pigs |
| title_fullStr | Phylogenetic analysis, biofilm production, and antimicrobial resistance profile of Escherichia coli isolated from slaughtered pigs |
| title_full_unstemmed | Phylogenetic analysis, biofilm production, and antimicrobial resistance profile of Escherichia coli isolated from slaughtered pigs |
| title_short | Phylogenetic analysis, biofilm production, and antimicrobial resistance profile of Escherichia coli isolated from slaughtered pigs |
| title_sort | phylogenetic analysis biofilm production and antimicrobial resistance profile of escherichia coli isolated from slaughtered pigs |
| topic | indústria suína colibacilose filogenia antibiograma multirresistência |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000401309&lng=en&tlng=en |
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