Enhanced trimethylamine metabolism and gut dysbiosis in type 2 diabetes mellitus with microalbumin
BackgroundAbnormal gut microbiota and blood trimethylamine-N-oxide (TMAO) metabolome have been reported in patients with type 2 diabetes mellitus (T2DM) and advanced diabetic nephropathy. This study aimed to investigate the gut microbiota profiles and a group of targeted urine metabolic characterist...
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| Format: | Article |
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Frontiers Media S.A.
2023-11-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2023.1257457/full |
| _version_ | 1797560901734236160 |
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| author | Lixia Huo Hui Li Ming Zhu Yang Liu Lingyan Ren Jia Hu Xiaoyi Wang |
| author_facet | Lixia Huo Hui Li Ming Zhu Yang Liu Lingyan Ren Jia Hu Xiaoyi Wang |
| author_sort | Lixia Huo |
| collection | DOAJ |
| description | BackgroundAbnormal gut microbiota and blood trimethylamine-N-oxide (TMAO) metabolome have been reported in patients with type 2 diabetes mellitus (T2DM) and advanced diabetic nephropathy. This study aimed to investigate the gut microbiota profiles and a group of targeted urine metabolic characteristics in T2DM patients with or without microalbuminuria, to determine the correlation between the gut microbiota composition, trimethylamine (TMA) metabolism, and the clinical features during progression of diabetic kidney disease (DKD)MethodsThis study included 26 T2DM patients with microalbuminuria (Micro), 26 T2DM patients with normoalbuminuria (Normo), and 15 healthy controls (HC). Urine and Fecal samples were detected using ultra performance liquid chromatography tandem mass spectrometry and 16S ribosomal DNA gene sequencing, respectively.ResultsThe TMAO/TMA ratio decreased gradually during the HC-Normo-Micro transition. The levels of TMA, choline and betaine were significantly different between the HC group and the T2DM patients belonging to both Normo and Micro groups. At the operational taxonomic unit (OTU) level, the gut microflora diversity was significantly reduced in the Micro groups compared to the HC groups and the Normo groups. Taxonomic analyses revealed significant consumption in the relative abundances of eight bacterial genera and significant enrichment of two bacterial genera during the HC-Normo-Micro transition. Furthermore, the relative abundances of six bacterial genera, namely, Ruminococcus_1, [Eubacterium]_ruminantium_group, Roseburia, Faecalibacterium, Fusicatenibacter and Coprococcus_3 exhibited significant differences, and were associated with elevated urinary albumin creatinine ratio (UACR), TMAO/TMA, TMA and its precursors in the Micro group compared with the other groups.ConclusionThe imbalance of gut microbiota has occurred in patients with early-stage DKD, and the consumption of short-chain fatty acid-producing bacteria were associated with the accumulation of TMA and UACR. |
| first_indexed | 2024-03-10T18:07:18Z |
| format | Article |
| id | doaj.art-26c3afa7817c434d8dcf06266127c2b5 |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-03-10T18:07:18Z |
| publishDate | 2023-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-26c3afa7817c434d8dcf06266127c2b52023-11-20T08:23:36ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922023-11-011410.3389/fendo.2023.12574571257457Enhanced trimethylamine metabolism and gut dysbiosis in type 2 diabetes mellitus with microalbuminLixia Huo0Hui Li1Ming Zhu2Yang Liu3Lingyan Ren4Jia Hu5Xiaoyi Wang6Huzhou Key Laboratory of Translational Medicine, The First Affiliated Hospital of Huzhou University, The First People’s Hospital, Huzhou, Zhejiang, ChinaDepartment of Environmental and Occupational Health, Center for Disease Control and Prevention, Huzhou, Zhejiang, ChinaDepartment of Nephrology, The First Affiliated Hospital of Huzhou University, The First People’s Hospital, Huzhou, Zhejiang, ChinaHuzhou Key Laboratory of Translational Medicine, The First Affiliated Hospital of Huzhou University, The First People’s Hospital, Huzhou, Zhejiang, ChinaDepartment of Nephrology, The First Affiliated Hospital of Huzhou University, The First People’s Hospital, Huzhou, Zhejiang, ChinaDepartment of Endocrinology, The First Affiliated Hospital of Huzhou University, The First People’s Hospital, Huzhou, Zhejiang, ChinaDepartment of Nephrology, The First Affiliated Hospital of Huzhou University, The First People’s Hospital, Huzhou, Zhejiang, ChinaBackgroundAbnormal gut microbiota and blood trimethylamine-N-oxide (TMAO) metabolome have been reported in patients with type 2 diabetes mellitus (T2DM) and advanced diabetic nephropathy. This study aimed to investigate the gut microbiota profiles and a group of targeted urine metabolic characteristics in T2DM patients with or without microalbuminuria, to determine the correlation between the gut microbiota composition, trimethylamine (TMA) metabolism, and the clinical features during progression of diabetic kidney disease (DKD)MethodsThis study included 26 T2DM patients with microalbuminuria (Micro), 26 T2DM patients with normoalbuminuria (Normo), and 15 healthy controls (HC). Urine and Fecal samples were detected using ultra performance liquid chromatography tandem mass spectrometry and 16S ribosomal DNA gene sequencing, respectively.ResultsThe TMAO/TMA ratio decreased gradually during the HC-Normo-Micro transition. The levels of TMA, choline and betaine were significantly different between the HC group and the T2DM patients belonging to both Normo and Micro groups. At the operational taxonomic unit (OTU) level, the gut microflora diversity was significantly reduced in the Micro groups compared to the HC groups and the Normo groups. Taxonomic analyses revealed significant consumption in the relative abundances of eight bacterial genera and significant enrichment of two bacterial genera during the HC-Normo-Micro transition. Furthermore, the relative abundances of six bacterial genera, namely, Ruminococcus_1, [Eubacterium]_ruminantium_group, Roseburia, Faecalibacterium, Fusicatenibacter and Coprococcus_3 exhibited significant differences, and were associated with elevated urinary albumin creatinine ratio (UACR), TMAO/TMA, TMA and its precursors in the Micro group compared with the other groups.ConclusionThe imbalance of gut microbiota has occurred in patients with early-stage DKD, and the consumption of short-chain fatty acid-producing bacteria were associated with the accumulation of TMA and UACR.https://www.frontiersin.org/articles/10.3389/fendo.2023.1257457/fulltype 2 diabetes mellitus with microalbuminuriagut microbiotatrimethylaminetrimethylamine-n-oxideurinary albumin creatinine ratio |
| spellingShingle | Lixia Huo Hui Li Ming Zhu Yang Liu Lingyan Ren Jia Hu Xiaoyi Wang Enhanced trimethylamine metabolism and gut dysbiosis in type 2 diabetes mellitus with microalbumin Frontiers in Endocrinology type 2 diabetes mellitus with microalbuminuria gut microbiota trimethylamine trimethylamine-n-oxide urinary albumin creatinine ratio |
| title | Enhanced trimethylamine metabolism and gut dysbiosis in type 2 diabetes mellitus with microalbumin |
| title_full | Enhanced trimethylamine metabolism and gut dysbiosis in type 2 diabetes mellitus with microalbumin |
| title_fullStr | Enhanced trimethylamine metabolism and gut dysbiosis in type 2 diabetes mellitus with microalbumin |
| title_full_unstemmed | Enhanced trimethylamine metabolism and gut dysbiosis in type 2 diabetes mellitus with microalbumin |
| title_short | Enhanced trimethylamine metabolism and gut dysbiosis in type 2 diabetes mellitus with microalbumin |
| title_sort | enhanced trimethylamine metabolism and gut dysbiosis in type 2 diabetes mellitus with microalbumin |
| topic | type 2 diabetes mellitus with microalbuminuria gut microbiota trimethylamine trimethylamine-n-oxide urinary albumin creatinine ratio |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2023.1257457/full |
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