Comparative genomics reveals electron transfer and syntrophic mechanisms differentiating methanotrophic and methanogenic archaea.
The anaerobic oxidation of methane coupled to sulfate reduction is a microbially mediated process requiring a syntrophic partnership between anaerobic methanotrophic (ANME) archaea and sulfate-reducing bacteria (SRB). Based on genome taxonomy, ANME lineages are polyphyletic within the phylum Halobac...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2022-01-01
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| Series: | PLoS Biology |
| Online Access: | https://doi.org/10.1371/journal.pbio.3001508 |
| _version_ | 1811292769577074688 |
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| author | Grayson L Chadwick Connor T Skennerton Rafael Laso-Pérez Andy O Leu Daan R Speth Hang Yu Connor Morgan-Lang Roland Hatzenpichler Danielle Goudeau Rex Malmstrom William J Brazelton Tanja Woyke Steven J Hallam Gene W Tyson Gunter Wegener Antje Boetius Victoria J Orphan |
| author_facet | Grayson L Chadwick Connor T Skennerton Rafael Laso-Pérez Andy O Leu Daan R Speth Hang Yu Connor Morgan-Lang Roland Hatzenpichler Danielle Goudeau Rex Malmstrom William J Brazelton Tanja Woyke Steven J Hallam Gene W Tyson Gunter Wegener Antje Boetius Victoria J Orphan |
| author_sort | Grayson L Chadwick |
| collection | DOAJ |
| description | The anaerobic oxidation of methane coupled to sulfate reduction is a microbially mediated process requiring a syntrophic partnership between anaerobic methanotrophic (ANME) archaea and sulfate-reducing bacteria (SRB). Based on genome taxonomy, ANME lineages are polyphyletic within the phylum Halobacterota, none of which have been isolated in pure culture. Here, we reconstruct 28 ANME genomes from environmental metagenomes and flow sorted syntrophic consortia. Together with a reanalysis of previously published datasets, these genomes enable a comparative analysis of all marine ANME clades. We review the genomic features that separate ANME from their methanogenic relatives and identify what differentiates ANME clades. Large multiheme cytochromes and bioenergetic complexes predicted to be involved in novel electron bifurcation reactions are well distributed and conserved in the ANME archaea, while significant variations in the anabolic C1 pathways exists between clades. Our analysis raises the possibility that methylotrophic methanogenesis may have evolved from a methanotrophic ancestor. |
| first_indexed | 2024-04-13T04:51:24Z |
| format | Article |
| id | doaj.art-26c3bee9c55c4549a91b05700e3a3b8d |
| institution | Directory Open Access Journal |
| issn | 1544-9173 1545-7885 |
| language | English |
| last_indexed | 2024-04-13T04:51:24Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Biology |
| spelling | doaj.art-26c3bee9c55c4549a91b05700e3a3b8d2022-12-22T03:01:40ZengPublic Library of Science (PLoS)PLoS Biology1544-91731545-78852022-01-01201e300150810.1371/journal.pbio.3001508Comparative genomics reveals electron transfer and syntrophic mechanisms differentiating methanotrophic and methanogenic archaea.Grayson L ChadwickConnor T SkennertonRafael Laso-PérezAndy O LeuDaan R SpethHang YuConnor Morgan-LangRoland HatzenpichlerDanielle GoudeauRex MalmstromWilliam J BrazeltonTanja WoykeSteven J HallamGene W TysonGunter WegenerAntje BoetiusVictoria J OrphanThe anaerobic oxidation of methane coupled to sulfate reduction is a microbially mediated process requiring a syntrophic partnership between anaerobic methanotrophic (ANME) archaea and sulfate-reducing bacteria (SRB). Based on genome taxonomy, ANME lineages are polyphyletic within the phylum Halobacterota, none of which have been isolated in pure culture. Here, we reconstruct 28 ANME genomes from environmental metagenomes and flow sorted syntrophic consortia. Together with a reanalysis of previously published datasets, these genomes enable a comparative analysis of all marine ANME clades. We review the genomic features that separate ANME from their methanogenic relatives and identify what differentiates ANME clades. Large multiheme cytochromes and bioenergetic complexes predicted to be involved in novel electron bifurcation reactions are well distributed and conserved in the ANME archaea, while significant variations in the anabolic C1 pathways exists between clades. Our analysis raises the possibility that methylotrophic methanogenesis may have evolved from a methanotrophic ancestor.https://doi.org/10.1371/journal.pbio.3001508 |
| spellingShingle | Grayson L Chadwick Connor T Skennerton Rafael Laso-Pérez Andy O Leu Daan R Speth Hang Yu Connor Morgan-Lang Roland Hatzenpichler Danielle Goudeau Rex Malmstrom William J Brazelton Tanja Woyke Steven J Hallam Gene W Tyson Gunter Wegener Antje Boetius Victoria J Orphan Comparative genomics reveals electron transfer and syntrophic mechanisms differentiating methanotrophic and methanogenic archaea. PLoS Biology |
| title | Comparative genomics reveals electron transfer and syntrophic mechanisms differentiating methanotrophic and methanogenic archaea. |
| title_full | Comparative genomics reveals electron transfer and syntrophic mechanisms differentiating methanotrophic and methanogenic archaea. |
| title_fullStr | Comparative genomics reveals electron transfer and syntrophic mechanisms differentiating methanotrophic and methanogenic archaea. |
| title_full_unstemmed | Comparative genomics reveals electron transfer and syntrophic mechanisms differentiating methanotrophic and methanogenic archaea. |
| title_short | Comparative genomics reveals electron transfer and syntrophic mechanisms differentiating methanotrophic and methanogenic archaea. |
| title_sort | comparative genomics reveals electron transfer and syntrophic mechanisms differentiating methanotrophic and methanogenic archaea |
| url | https://doi.org/10.1371/journal.pbio.3001508 |
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