<i>Mycobacterium pseudoshottsii</i> in Mediterranean Fish Farms: New Trouble for European Aquaculture?
<i>Mycobacterium pseudoshottsii</i>, a slow-growing mycobacterium closely related to <i>M. marinum</i>, has been isolated only in wild fish in the United States and in Japanese fish farms to date. Here, we report cases of mortality in three farmed fish species (<i>Dicen...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-07-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/9/8/610 |
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| author | Davide Mugetti Katia Varello Andrea Gustinelli Paolo Pastorino Vasco Menconi Daniela Florio Maria Letizia Fioravanti Elena Bozzetta Simona Zoppi Alessandro Dondo Marino Prearo |
| author_facet | Davide Mugetti Katia Varello Andrea Gustinelli Paolo Pastorino Vasco Menconi Daniela Florio Maria Letizia Fioravanti Elena Bozzetta Simona Zoppi Alessandro Dondo Marino Prearo |
| author_sort | Davide Mugetti |
| collection | DOAJ |
| description | <i>Mycobacterium pseudoshottsii</i>, a slow-growing mycobacterium closely related to <i>M. marinum</i>, has been isolated only in wild fish in the United States and in Japanese fish farms to date. Here, we report cases of mortality in three farmed fish species (<i>Dicentrarchus labrax</i>, <i>Sparus aurata</i>, and <i>Sciaenops ocellatus</i>) caused by <i>M. pseudoshottsii</i> in Italy. Samples underwent necropsy, histology, and culture with pathogen identification based on PCR and sequencing of housekeeping genes (16S rRNA, <i>hsp65</i>, <i>rpoB</i>). Multifocal to coalescing granulomatous and necrotizing inflammation with acid-fast bacilli were observed in the parenchymatous organs, from which <i>M. pseudoshottsii</i> was isolated and identified. Phylogenetic analysis confirmed the results of gene sequencing and allowed subdivision of the isolates into three distinct groups. <i>M. pseudoshottsii</i> poses a potential threat for Mediterranean aquaculture. Its origin in the area under study needs to be clarified, as well as the threat to the farmed fish species. |
| first_indexed | 2024-03-10T18:10:49Z |
| format | Article |
| id | doaj.art-26c7462579b542de8b7a48d8cf29ad59 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T18:10:49Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-26c7462579b542de8b7a48d8cf29ad592023-11-20T08:03:55ZengMDPI AGPathogens2076-08172020-07-019861010.3390/pathogens9080610<i>Mycobacterium pseudoshottsii</i> in Mediterranean Fish Farms: New Trouble for European Aquaculture?Davide Mugetti0Katia Varello1Andrea Gustinelli2Paolo Pastorino3Vasco Menconi4Daniela Florio5Maria Letizia Fioravanti6Elena Bozzetta7Simona Zoppi8Alessandro Dondo9Marino Prearo10Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, 10154 Torino, ItalyIstituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, 10154 Torino, ItalyDipartimento di Scienze Mediche Veterinarie, Alma Mater Studiorum, 40064 Ozzano dell’Emilia (BO), ItalyIstituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, 10154 Torino, ItalyIstituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, 10154 Torino, ItalyDipartimento di Scienze Mediche Veterinarie, Alma Mater Studiorum, 40064 Ozzano dell’Emilia (BO), ItalyDipartimento di Scienze Mediche Veterinarie, Alma Mater Studiorum, 40064 Ozzano dell’Emilia (BO), ItalyIstituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, 10154 Torino, ItalyIstituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, 10154 Torino, ItalyIstituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, 10154 Torino, ItalyIstituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, 10154 Torino, Italy<i>Mycobacterium pseudoshottsii</i>, a slow-growing mycobacterium closely related to <i>M. marinum</i>, has been isolated only in wild fish in the United States and in Japanese fish farms to date. Here, we report cases of mortality in three farmed fish species (<i>Dicentrarchus labrax</i>, <i>Sparus aurata</i>, and <i>Sciaenops ocellatus</i>) caused by <i>M. pseudoshottsii</i> in Italy. Samples underwent necropsy, histology, and culture with pathogen identification based on PCR and sequencing of housekeeping genes (16S rRNA, <i>hsp65</i>, <i>rpoB</i>). Multifocal to coalescing granulomatous and necrotizing inflammation with acid-fast bacilli were observed in the parenchymatous organs, from which <i>M. pseudoshottsii</i> was isolated and identified. Phylogenetic analysis confirmed the results of gene sequencing and allowed subdivision of the isolates into three distinct groups. <i>M. pseudoshottsii</i> poses a potential threat for Mediterranean aquaculture. Its origin in the area under study needs to be clarified, as well as the threat to the farmed fish species.https://www.mdpi.com/2076-0817/9/8/610atypical mycobacteriaslow-growing mycobacteria<i>Mycobacterium marinum</i> complexemerging diseasesEuropean sea bassgilthead sea bream |
| spellingShingle | Davide Mugetti Katia Varello Andrea Gustinelli Paolo Pastorino Vasco Menconi Daniela Florio Maria Letizia Fioravanti Elena Bozzetta Simona Zoppi Alessandro Dondo Marino Prearo <i>Mycobacterium pseudoshottsii</i> in Mediterranean Fish Farms: New Trouble for European Aquaculture? Pathogens atypical mycobacteria slow-growing mycobacteria <i>Mycobacterium marinum</i> complex emerging diseases European sea bass gilthead sea bream |
| title | <i>Mycobacterium pseudoshottsii</i> in Mediterranean Fish Farms: New Trouble for European Aquaculture? |
| title_full | <i>Mycobacterium pseudoshottsii</i> in Mediterranean Fish Farms: New Trouble for European Aquaculture? |
| title_fullStr | <i>Mycobacterium pseudoshottsii</i> in Mediterranean Fish Farms: New Trouble for European Aquaculture? |
| title_full_unstemmed | <i>Mycobacterium pseudoshottsii</i> in Mediterranean Fish Farms: New Trouble for European Aquaculture? |
| title_short | <i>Mycobacterium pseudoshottsii</i> in Mediterranean Fish Farms: New Trouble for European Aquaculture? |
| title_sort | i mycobacterium pseudoshottsii i in mediterranean fish farms new trouble for european aquaculture |
| topic | atypical mycobacteria slow-growing mycobacteria <i>Mycobacterium marinum</i> complex emerging diseases European sea bass gilthead sea bream |
| url | https://www.mdpi.com/2076-0817/9/8/610 |
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