Investigations of genotoxic potential of levamisole hydrochloride in bone marrow cells of Wistar rats
An experiment was performed under in vivo conditions on bone marrow cells of Wistar rats. The following doses of levamisole hydrochloride were tested: a therapeutic dose of 2.2 mg/kg bm, a dose of 4.4 mg/kg bm, LD50 -25% mg/kg bm, and LD50 -75% mg/kg bm. We followed the effect of levamisole hydrochl...
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| Format: | Article |
| Language: | English |
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Faculty of Veterinary Medicine, Belgrade
2006-01-01
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| Series: | Veterinarski Glasnik |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0350-2457/2006/0350-24570602003K.pdf |
| _version_ | 1819279445509275648 |
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| author | Kulić Milan Stanimirović Zoran Marković Biljana Ristić Siniša |
| author_facet | Kulić Milan Stanimirović Zoran Marković Biljana Ristić Siniša |
| author_sort | Kulić Milan |
| collection | DOAJ |
| description | An experiment was performed under in vivo conditions on bone marrow cells of Wistar rats. The following doses of levamisole hydrochloride were tested: a therapeutic dose of 2.2 mg/kg bm, a dose of 4.4 mg/kg bm, LD50 -25% mg/kg bm, and LD50 -75% mg/kg bm. We followed the effect of levamisole hydrochloride on kinetics of the cell cycle and the appearance of structural and numeric changes in chromosomes in bone marrow cells. The therapeutic dose of levamisole of 2.2 mg/kg bm exhibited a capability to increase mitotic activity in the observed cells, thus confirming knowledge of the immunostimulative effect of this dose of the medicine under in vivo conditions. The other tested doses of levamisole in this experiment, observed in comparison with the control group, had an opposite effect, namely, they caused a reduction in the mitotic activity of bone marrow cells. All the examined doses in vivo exhibited the ability to induce numeric (aneuploid and polyploid) and structural (lesions, breaks and insertions) chromosomal aberrations. It can be concluded on the grounds of these findings that the examined doses have a genotoxic effect. |
| first_indexed | 2024-12-24T00:28:01Z |
| format | Article |
| id | doaj.art-26cd85ffbcc1419790ae39d549f9cd99 |
| institution | Directory Open Access Journal |
| issn | 0350-2457 2406-0771 |
| language | English |
| last_indexed | 2024-12-24T00:28:01Z |
| publishDate | 2006-01-01 |
| publisher | Faculty of Veterinary Medicine, Belgrade |
| record_format | Article |
| series | Veterinarski Glasnik |
| spelling | doaj.art-26cd85ffbcc1419790ae39d549f9cd992022-12-21T17:24:21ZengFaculty of Veterinary Medicine, BelgradeVeterinarski Glasnik0350-24572406-07712006-01-01601-23910.2298/VETGL0602003K0350-24570602003KInvestigations of genotoxic potential of levamisole hydrochloride in bone marrow cells of Wistar ratsKulić Milan0Stanimirović Zoran1Marković Biljana2Ristić Siniša3Katedra za humanu genetiku, Medicinski fakultet, Foča, BIHKatedra za biologiju, Fakultet veterinarske medicine, BeogradKatedra za biologiju, Fakultet veterinarske medicine, BeogradKatedra za fiziologiju, Medicinski fakultet, Foča, BIHAn experiment was performed under in vivo conditions on bone marrow cells of Wistar rats. The following doses of levamisole hydrochloride were tested: a therapeutic dose of 2.2 mg/kg bm, a dose of 4.4 mg/kg bm, LD50 -25% mg/kg bm, and LD50 -75% mg/kg bm. We followed the effect of levamisole hydrochloride on kinetics of the cell cycle and the appearance of structural and numeric changes in chromosomes in bone marrow cells. The therapeutic dose of levamisole of 2.2 mg/kg bm exhibited a capability to increase mitotic activity in the observed cells, thus confirming knowledge of the immunostimulative effect of this dose of the medicine under in vivo conditions. The other tested doses of levamisole in this experiment, observed in comparison with the control group, had an opposite effect, namely, they caused a reduction in the mitotic activity of bone marrow cells. All the examined doses in vivo exhibited the ability to induce numeric (aneuploid and polyploid) and structural (lesions, breaks and insertions) chromosomal aberrations. It can be concluded on the grounds of these findings that the examined doses have a genotoxic effect.http://www.doiserbia.nb.rs/img/doi/0350-2457/2006/0350-24570602003K.pdfgenotoxitycytotoxicityaneuploidpolyploid lesionsbreaksinsertions |
| spellingShingle | Kulić Milan Stanimirović Zoran Marković Biljana Ristić Siniša Investigations of genotoxic potential of levamisole hydrochloride in bone marrow cells of Wistar rats Veterinarski Glasnik genotoxity cytotoxicity aneuploid polyploid lesions breaks insertions |
| title | Investigations of genotoxic potential of levamisole hydrochloride in bone marrow cells of Wistar rats |
| title_full | Investigations of genotoxic potential of levamisole hydrochloride in bone marrow cells of Wistar rats |
| title_fullStr | Investigations of genotoxic potential of levamisole hydrochloride in bone marrow cells of Wistar rats |
| title_full_unstemmed | Investigations of genotoxic potential of levamisole hydrochloride in bone marrow cells of Wistar rats |
| title_short | Investigations of genotoxic potential of levamisole hydrochloride in bone marrow cells of Wistar rats |
| title_sort | investigations of genotoxic potential of levamisole hydrochloride in bone marrow cells of wistar rats |
| topic | genotoxity cytotoxicity aneuploid polyploid lesions breaks insertions |
| url | http://www.doiserbia.nb.rs/img/doi/0350-2457/2006/0350-24570602003K.pdf |
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