Anatomy of hypothalamic and diencephalic nuclei involved in seasonal fertility regulation in ewes
In this study, we describe in detail the anatomy of nuclei involved in seasonal fertility regulation (SFR) in ewes. For this purpose, the intergeniculate leaflet of the visual thalamus, the caudal hypothalamic arcuate nucleus, and suprachiasmatic, paraventricular and supraoptic nuclei of the rostral...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2023.1101024/full |
| _version_ | 1797904963704193024 |
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| author | Miguel Merchán Miguel Merchán Miguel Merchán Rafael Coveñas Rafael Coveñas Ignacio Plaza José Alfonso Abecia Carlos Palacios |
| author_facet | Miguel Merchán Miguel Merchán Miguel Merchán Rafael Coveñas Rafael Coveñas Ignacio Plaza José Alfonso Abecia Carlos Palacios |
| author_sort | Miguel Merchán |
| collection | DOAJ |
| description | In this study, we describe in detail the anatomy of nuclei involved in seasonal fertility regulation (SFR) in ewes. For this purpose, the intergeniculate leaflet of the visual thalamus, the caudal hypothalamic arcuate nucleus, and suprachiasmatic, paraventricular and supraoptic nuclei of the rostral hypothalamus were morphometrically and qualitatively analyzed in Nissl-stained serial sections, in the three anatomical planes. In addition, data were collected on calcium-binding proteins and cell phenotypes after immunostaining alternate serial sections for calretinin, parvalbumin and calbindin. For a complete neuroanatomical study, glial architecture was assessed by immunostaining and analyzing alternate sections for glial fibrillary acidic protein (GFAP) and ionized calcium-binding adapter molecule 1 (IBA1). The results showed a strong microglial and astroglia reaction around the hypothalamic nuclei of interest and around the whole 3rd ventricle of the ewe brain. Moreover, we correlated cytoarchitectonic coordinates of panoramic serial sections with their macroscopic localization and extension in midline sagittal-sectioned whole brain to provide guidelines for microdissecting nuclei involved in SFR. |
| first_indexed | 2024-04-10T09:58:35Z |
| format | Article |
| id | doaj.art-26d07bd0e4fc423e88951fe9e01af13f |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-04-10T09:58:35Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-26d07bd0e4fc423e88951fe9e01af13f2023-02-16T11:35:20ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692023-02-011010.3389/fvets.2023.11010241101024Anatomy of hypothalamic and diencephalic nuclei involved in seasonal fertility regulation in ewesMiguel Merchán0Miguel Merchán1Miguel Merchán2Rafael Coveñas3Rafael Coveñas4Ignacio Plaza5José Alfonso Abecia6Carlos Palacios7Animal Production Area, Department of Construction and Agronomy, Faculty of Agricultural and Environmental Sciences, University of Salamanca, Salamanca, SpainLaboratory of Neuroanatomy of the Peptidergic Systems, Institute for Neuroscience of Castilla y León (INCYL), University of Salamanca, Salamanca, SpainRecognized Research Group - Molecular Bases of Development (Grupo de Investigación Reconocido - Bases Moleculares del Desarrollo - GIR-BMD), University of Salamanca, Salamanca, SpainLaboratory of Neuroanatomy of the Peptidergic Systems, Institute for Neuroscience of Castilla y León (INCYL), University of Salamanca, Salamanca, SpainRecognized Research Group - Molecular Bases of Development (Grupo de Investigación Reconocido - Bases Moleculares del Desarrollo - GIR-BMD), University of Salamanca, Salamanca, SpainAuditory Neuroplasticity Laboratory, Institute for Neuroscience of Castilla y León (INCYL), University of Salamanca, Salamanca, SpainEnvironmental Science Institute (IUCA), University of Zaragoza, Zaragoza, SpainAnimal Production Area, Department of Construction and Agronomy, Faculty of Agricultural and Environmental Sciences, University of Salamanca, Salamanca, SpainIn this study, we describe in detail the anatomy of nuclei involved in seasonal fertility regulation (SFR) in ewes. For this purpose, the intergeniculate leaflet of the visual thalamus, the caudal hypothalamic arcuate nucleus, and suprachiasmatic, paraventricular and supraoptic nuclei of the rostral hypothalamus were morphometrically and qualitatively analyzed in Nissl-stained serial sections, in the three anatomical planes. In addition, data were collected on calcium-binding proteins and cell phenotypes after immunostaining alternate serial sections for calretinin, parvalbumin and calbindin. For a complete neuroanatomical study, glial architecture was assessed by immunostaining and analyzing alternate sections for glial fibrillary acidic protein (GFAP) and ionized calcium-binding adapter molecule 1 (IBA1). The results showed a strong microglial and astroglia reaction around the hypothalamic nuclei of interest and around the whole 3rd ventricle of the ewe brain. Moreover, we correlated cytoarchitectonic coordinates of panoramic serial sections with their macroscopic localization and extension in midline sagittal-sectioned whole brain to provide guidelines for microdissecting nuclei involved in SFR.https://www.frontiersin.org/articles/10.3389/fvets.2023.1101024/fullcalbindincalretininparvalbuminGFAPIBA1Nissl staining |
| spellingShingle | Miguel Merchán Miguel Merchán Miguel Merchán Rafael Coveñas Rafael Coveñas Ignacio Plaza José Alfonso Abecia Carlos Palacios Anatomy of hypothalamic and diencephalic nuclei involved in seasonal fertility regulation in ewes Frontiers in Veterinary Science calbindin calretinin parvalbumin GFAP IBA1 Nissl staining |
| title | Anatomy of hypothalamic and diencephalic nuclei involved in seasonal fertility regulation in ewes |
| title_full | Anatomy of hypothalamic and diencephalic nuclei involved in seasonal fertility regulation in ewes |
| title_fullStr | Anatomy of hypothalamic and diencephalic nuclei involved in seasonal fertility regulation in ewes |
| title_full_unstemmed | Anatomy of hypothalamic and diencephalic nuclei involved in seasonal fertility regulation in ewes |
| title_short | Anatomy of hypothalamic and diencephalic nuclei involved in seasonal fertility regulation in ewes |
| title_sort | anatomy of hypothalamic and diencephalic nuclei involved in seasonal fertility regulation in ewes |
| topic | calbindin calretinin parvalbumin GFAP IBA1 Nissl staining |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2023.1101024/full |
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