Hypoxylonol F Isolated from <i>Annulohypoxylon annulatum</i> Improves Insulin Secretion by Regulating Pancreatic β-cell Metabolism
Insulin plays a key role in glucose homeostasis and is hence used to treat hyperglycemia, the main characteristic of diabetes mellitus. <i>Annulohypoxylon annulatum</i> is an inedible ball-shaped wood-rotting fungus, and hypoxylon F is one of the major compounds of <i>A. annulatum&...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2019-08-01
|
| Series: | Biomolecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-273X/9/8/335 |
| _version_ | 1818453675351212032 |
|---|---|
| author | Dahae Lee Buyng Su Hwang Pilju Choi Taejung Kim Youngseok Kim Bong Geun Song Noriko Yamabe Gwi Seo Hwang Ki Sung Kang Jungyeob Ham |
| author_facet | Dahae Lee Buyng Su Hwang Pilju Choi Taejung Kim Youngseok Kim Bong Geun Song Noriko Yamabe Gwi Seo Hwang Ki Sung Kang Jungyeob Ham |
| author_sort | Dahae Lee |
| collection | DOAJ |
| description | Insulin plays a key role in glucose homeostasis and is hence used to treat hyperglycemia, the main characteristic of diabetes mellitus. <i>Annulohypoxylon annulatum</i> is an inedible ball-shaped wood-rotting fungus, and hypoxylon F is one of the major compounds of <i>A. annulatum</i>. The aim of this study is to evaluate the effects of hypoxylonol F isolated from <i>A. annulatum</i> on insulin secretion in INS-1 pancreatic β-cells and demonstrate the molecular mechanisms involved. Glucose-stimulated insulin secretion (GSIS) values were evaluated using a rat insulin ELISA kit. Moreover, the expression of proteins related to pancreatic β-cell metabolism and insulin secretion was evaluated using Western blotting. Hypoxylonol F isolated from <i>A. annulatum</i> was found to significantly enhance glucose-stimulated insulin secretion without inducing cytotoxicity. Additionally, hypoxylonol F enhanced insulin receptor substrate-2 (IRS-2) levels and activated the phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) pathway. Interestingly, it also modulated the expression of peroxisome proliferator-activated receptor γ (PPARγ) and pancreatic and duodenal homeobox 1 (PDX-1). Our findings showed that <i>A. annulatum</i> and its bioactive compounds are capable of improving insulin secretion by pancreatic β-cells. This suggests that <i>A. annulatum</i> can be used as a therapeutic agent to treat diabetes. |
| first_indexed | 2024-12-14T21:42:45Z |
| format | Article |
| id | doaj.art-26d16dc710d443f99d33e13c88cc7e2f |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-12-14T21:42:45Z |
| publishDate | 2019-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-26d16dc710d443f99d33e13c88cc7e2f2022-12-21T22:46:25ZengMDPI AGBiomolecules2218-273X2019-08-019833510.3390/biom9080335biom9080335Hypoxylonol F Isolated from <i>Annulohypoxylon annulatum</i> Improves Insulin Secretion by Regulating Pancreatic β-cell MetabolismDahae Lee0Buyng Su Hwang1Pilju Choi2Taejung Kim3Youngseok Kim4Bong Geun Song5Noriko Yamabe6Gwi Seo Hwang7Ki Sung Kang8Jungyeob Ham9School of Pharmacy, Sungkyunkwan University, Suwon 16419, KoreaFreshwater Bioresources Utilization Bureau, Nakdonggang National Institute of Biological Resources, Sangju 37242, KoreaNatural Products Research Institute, Korea Institute of Science and Technology, Gangneung 25451, KoreaNatural Products Research Institute, Korea Institute of Science and Technology, Gangneung 25451, KoreaNatural Products Research Institute, Korea Institute of Science and Technology, Gangneung 25451, KoreaNatural Products Research Institute, Korea Institute of Science and Technology, Gangneung 25451, KoreaCollege of Korean Medicine, Gachon University, Seongnam 13120, KoreaCollege of Korean Medicine, Gachon University, Seongnam 13120, KoreaCollege of Korean Medicine, Gachon University, Seongnam 13120, KoreaFreshwater Bioresources Utilization Bureau, Nakdonggang National Institute of Biological Resources, Sangju 37242, KoreaInsulin plays a key role in glucose homeostasis and is hence used to treat hyperglycemia, the main characteristic of diabetes mellitus. <i>Annulohypoxylon annulatum</i> is an inedible ball-shaped wood-rotting fungus, and hypoxylon F is one of the major compounds of <i>A. annulatum</i>. The aim of this study is to evaluate the effects of hypoxylonol F isolated from <i>A. annulatum</i> on insulin secretion in INS-1 pancreatic β-cells and demonstrate the molecular mechanisms involved. Glucose-stimulated insulin secretion (GSIS) values were evaluated using a rat insulin ELISA kit. Moreover, the expression of proteins related to pancreatic β-cell metabolism and insulin secretion was evaluated using Western blotting. Hypoxylonol F isolated from <i>A. annulatum</i> was found to significantly enhance glucose-stimulated insulin secretion without inducing cytotoxicity. Additionally, hypoxylonol F enhanced insulin receptor substrate-2 (IRS-2) levels and activated the phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) pathway. Interestingly, it also modulated the expression of peroxisome proliferator-activated receptor γ (PPARγ) and pancreatic and duodenal homeobox 1 (PDX-1). Our findings showed that <i>A. annulatum</i> and its bioactive compounds are capable of improving insulin secretion by pancreatic β-cells. This suggests that <i>A. annulatum</i> can be used as a therapeutic agent to treat diabetes.https://www.mdpi.com/2218-273X/9/8/335<i>Annulohypoxylon annulatum</i>insulinPI3KAktPPARγPDX-1 |
| spellingShingle | Dahae Lee Buyng Su Hwang Pilju Choi Taejung Kim Youngseok Kim Bong Geun Song Noriko Yamabe Gwi Seo Hwang Ki Sung Kang Jungyeob Ham Hypoxylonol F Isolated from <i>Annulohypoxylon annulatum</i> Improves Insulin Secretion by Regulating Pancreatic β-cell Metabolism Biomolecules <i>Annulohypoxylon annulatum</i> insulin PI3K Akt PPARγ PDX-1 |
| title | Hypoxylonol F Isolated from <i>Annulohypoxylon annulatum</i> Improves Insulin Secretion by Regulating Pancreatic β-cell Metabolism |
| title_full | Hypoxylonol F Isolated from <i>Annulohypoxylon annulatum</i> Improves Insulin Secretion by Regulating Pancreatic β-cell Metabolism |
| title_fullStr | Hypoxylonol F Isolated from <i>Annulohypoxylon annulatum</i> Improves Insulin Secretion by Regulating Pancreatic β-cell Metabolism |
| title_full_unstemmed | Hypoxylonol F Isolated from <i>Annulohypoxylon annulatum</i> Improves Insulin Secretion by Regulating Pancreatic β-cell Metabolism |
| title_short | Hypoxylonol F Isolated from <i>Annulohypoxylon annulatum</i> Improves Insulin Secretion by Regulating Pancreatic β-cell Metabolism |
| title_sort | hypoxylonol f isolated from i annulohypoxylon annulatum i improves insulin secretion by regulating pancreatic β cell metabolism |
| topic | <i>Annulohypoxylon annulatum</i> insulin PI3K Akt PPARγ PDX-1 |
| url | https://www.mdpi.com/2218-273X/9/8/335 |
| work_keys_str_mv | AT dahaelee hypoxylonolfisolatedfromiannulohypoxylonannulatumiimprovesinsulinsecretionbyregulatingpancreaticbcellmetabolism AT buyngsuhwang hypoxylonolfisolatedfromiannulohypoxylonannulatumiimprovesinsulinsecretionbyregulatingpancreaticbcellmetabolism AT piljuchoi hypoxylonolfisolatedfromiannulohypoxylonannulatumiimprovesinsulinsecretionbyregulatingpancreaticbcellmetabolism AT taejungkim hypoxylonolfisolatedfromiannulohypoxylonannulatumiimprovesinsulinsecretionbyregulatingpancreaticbcellmetabolism AT youngseokkim hypoxylonolfisolatedfromiannulohypoxylonannulatumiimprovesinsulinsecretionbyregulatingpancreaticbcellmetabolism AT bonggeunsong hypoxylonolfisolatedfromiannulohypoxylonannulatumiimprovesinsulinsecretionbyregulatingpancreaticbcellmetabolism AT norikoyamabe hypoxylonolfisolatedfromiannulohypoxylonannulatumiimprovesinsulinsecretionbyregulatingpancreaticbcellmetabolism AT gwiseohwang hypoxylonolfisolatedfromiannulohypoxylonannulatumiimprovesinsulinsecretionbyregulatingpancreaticbcellmetabolism AT kisungkang hypoxylonolfisolatedfromiannulohypoxylonannulatumiimprovesinsulinsecretionbyregulatingpancreaticbcellmetabolism AT jungyeobham hypoxylonolfisolatedfromiannulohypoxylonannulatumiimprovesinsulinsecretionbyregulatingpancreaticbcellmetabolism |