Investigation of phytochemical composition and bioactivity evaluation of extracts from Myrsine umbellata Mart.
Abstract The objective of the study was to carry out phytochemical prospection through colorimetric tests to determine the groups of secondary metabolites and also to determine the total content of phenolic compounds (TPC) present in plant extracts methanol (ME), ethyl acetate (EAE), hexane (HE) and...
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| Format: | Article |
| Language: | English |
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Instituto Internacional de Ecologia
2024-03-01
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| Series: | Brazilian Journal of Biology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842024000101103&lng=en&tlng=en |
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| author | L. V. Laskoski J. M. Batista D. M. Bandeira J. M. Corrêa J. Rosset L. H. S. M. Conceição F. G. S. Pinto |
| author_facet | L. V. Laskoski J. M. Batista D. M. Bandeira J. M. Corrêa J. Rosset L. H. S. M. Conceição F. G. S. Pinto |
| author_sort | L. V. Laskoski |
| collection | DOAJ |
| description | Abstract The objective of the study was to carry out phytochemical prospection through colorimetric tests to determine the groups of secondary metabolites and also to determine the total content of phenolic compounds (TPC) present in plant extracts methanol (ME), ethyl acetate (EAE), hexane (HE) and dichloromethane (DE) from the leaves of Myrsine umbellata, as well as to investigate the antimicrobial activity against twelve standard ATCC strains by the broth microdilution technique; the antioxidant potential by the DPPH method and the ABTS method and the antibiofilm potential on the biofilm biomass of standard bacteria by the crystal violet technique and tetrazolium salt reduction (MTT) assay. Phytochemical prospection detected the presence of saponins, steroids, alkaloids, anthocyanins, anthocyanidins, flavonoids, and tannins. The results of the quantitative phytochemical estimation revealed a higher content of total phenolics in DE (280.24 ± 0.037 µM GAE g ext. -1) followed by ME (159.01 ± 0.031 µM GAE g ext. -1). The ME showed the best biological activities when compared to the other extracts tested. We observed antimicrobial activity against Gram-positive Staphylococcus epidermidis strain (MIC 3.12 and MBC 6.25), antioxidant percentage of 92.58% against the DPPH radical and 420.31 µM Trolox g ext. -1 against the ABTS radical, finally showed antibiofilm action against Gram-positive strain Staphylococcus aureus, with eradication of the biomass in 92.58%. The results suggest that EM from M. umbellata represents an alternative source of plant bioactives for the development of natura products. |
| first_indexed | 2024-03-07T15:41:22Z |
| format | Article |
| id | doaj.art-26d1732fe43943d3bfa16ba81bd5b9ff |
| institution | Directory Open Access Journal |
| issn | 1678-4375 |
| language | English |
| last_indexed | 2024-03-07T15:41:22Z |
| publishDate | 2024-03-01 |
| publisher | Instituto Internacional de Ecologia |
| record_format | Article |
| series | Brazilian Journal of Biology |
| spelling | doaj.art-26d1732fe43943d3bfa16ba81bd5b9ff2024-03-05T07:42:22ZengInstituto Internacional de EcologiaBrazilian Journal of Biology1678-43752024-03-018410.1590/1519-6984.276871Investigation of phytochemical composition and bioactivity evaluation of extracts from Myrsine umbellata Mart.L. V. Laskoskihttps://orcid.org/0000-0001-8185-3790J. M. Batistahttps://orcid.org/0000-0002-1699-9643D. M. Bandeirahttps://orcid.org/0000-0001-5956-7210J. M. Corrêahttps://orcid.org/0000-0003-4526-0060J. Rossethttps://orcid.org/0000-0002-8348-0649L. H. S. M. Conceiçãohttps://orcid.org/0000-0002-9804-3476F. G. S. Pintohttps://orcid.org/0000-0002-0486-8486Abstract The objective of the study was to carry out phytochemical prospection through colorimetric tests to determine the groups of secondary metabolites and also to determine the total content of phenolic compounds (TPC) present in plant extracts methanol (ME), ethyl acetate (EAE), hexane (HE) and dichloromethane (DE) from the leaves of Myrsine umbellata, as well as to investigate the antimicrobial activity against twelve standard ATCC strains by the broth microdilution technique; the antioxidant potential by the DPPH method and the ABTS method and the antibiofilm potential on the biofilm biomass of standard bacteria by the crystal violet technique and tetrazolium salt reduction (MTT) assay. Phytochemical prospection detected the presence of saponins, steroids, alkaloids, anthocyanins, anthocyanidins, flavonoids, and tannins. The results of the quantitative phytochemical estimation revealed a higher content of total phenolics in DE (280.24 ± 0.037 µM GAE g ext. -1) followed by ME (159.01 ± 0.031 µM GAE g ext. -1). The ME showed the best biological activities when compared to the other extracts tested. We observed antimicrobial activity against Gram-positive Staphylococcus epidermidis strain (MIC 3.12 and MBC 6.25), antioxidant percentage of 92.58% against the DPPH radical and 420.31 µM Trolox g ext. -1 against the ABTS radical, finally showed antibiofilm action against Gram-positive strain Staphylococcus aureus, with eradication of the biomass in 92.58%. The results suggest that EM from M. umbellata represents an alternative source of plant bioactives for the development of natura products.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842024000101103&lng=en&tlng=encapororocabiological activitiesbiofilmbioactive compounds |
| spellingShingle | L. V. Laskoski J. M. Batista D. M. Bandeira J. M. Corrêa J. Rosset L. H. S. M. Conceição F. G. S. Pinto Investigation of phytochemical composition and bioactivity evaluation of extracts from Myrsine umbellata Mart. Brazilian Journal of Biology capororoca biological activities biofilm bioactive compounds |
| title | Investigation of phytochemical composition and bioactivity evaluation of extracts from Myrsine umbellata Mart. |
| title_full | Investigation of phytochemical composition and bioactivity evaluation of extracts from Myrsine umbellata Mart. |
| title_fullStr | Investigation of phytochemical composition and bioactivity evaluation of extracts from Myrsine umbellata Mart. |
| title_full_unstemmed | Investigation of phytochemical composition and bioactivity evaluation of extracts from Myrsine umbellata Mart. |
| title_short | Investigation of phytochemical composition and bioactivity evaluation of extracts from Myrsine umbellata Mart. |
| title_sort | investigation of phytochemical composition and bioactivity evaluation of extracts from myrsine umbellata mart |
| topic | capororoca biological activities biofilm bioactive compounds |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842024000101103&lng=en&tlng=en |
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