Identification, Classification and Characterization of bZIP Transcription Factor Family Members in <i>Pinus massoniana</i> Lamb.
Basic leucine zipper (bZIP) transcription factors (TFs) are ubiquitous in eukaryotes. Members of this family play significant roles in the regulation of plant growth, signal transduction, and various stresses. To date, bZIP TFs have been extensively studied in various plants, but there is little inf...
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| Format: | Article |
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MDPI AG
2023-01-01
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| Series: | Forests |
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| Online Access: | https://www.mdpi.com/1999-4907/14/1/155 |
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| author | Mengyang Zhang Peihuang Zhu Romaric Hippolyte Agassin Sheng Yao Dengbao Wang Zichen Huang Chi Zhang Qingqing Hao Kongshu Ji |
| author_facet | Mengyang Zhang Peihuang Zhu Romaric Hippolyte Agassin Sheng Yao Dengbao Wang Zichen Huang Chi Zhang Qingqing Hao Kongshu Ji |
| author_sort | Mengyang Zhang |
| collection | DOAJ |
| description | Basic leucine zipper (bZIP) transcription factors (TFs) are ubiquitous in eukaryotes. Members of this family play significant roles in the regulation of plant growth, signal transduction, and various stresses. To date, bZIP TFs have been extensively studied in various plants, but there is little information about them in <i>Pinus massoniana</i> Lamb. In this study, 55 bZIP TFs were identified based on data from four different <i>P. massoniana</i> transcriptomes, and a systematic analysis was performed. According to the phylogenetic results, <i>P. massoniana</i> bZIP TFs were divided into 11 groups. Each bZIP protein contained a highly conserved bZIP domain, and the numbers and types of motifs were similar in the same group. The PmbZIPs were nuclear localization proteins. Based on the pine wood nematode inoculation transcriptome, the transcriptional profiles revealed that 25 <i>PmbZIP</i> genes could respond to pine wood nematodes at different levels. Genes <i>PmbZIP3, PmbZIP4, PmbZIP8, PmbZIP20,</i> and <i>PmbZIP23</i> were selected to be upregulated in the process of inoculation with pine wood nematodes. These five genes had different expression levels in different tissues and were responsive to the related treatment conditions. Transcriptional activity analysis showed that PmbZIP3 and PmbZIP8 were transcriptional activators; PmbZIP4, PmbZIP20 and PmbZIP23 were transcriptional repressors. These findings provide preliminary information on PmbZIP TFs, which is helpful for further study of other physiological functions of bZIP TFs in <i>P. massoniana</i>. |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-09T12:40:37Z |
| publishDate | 2023-01-01 |
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| series | Forests |
| spelling | doaj.art-26d183634c91426f9eb45f6118c896152023-11-30T22:18:30ZengMDPI AGForests1999-49072023-01-0114115510.3390/f14010155Identification, Classification and Characterization of bZIP Transcription Factor Family Members in <i>Pinus massoniana</i> Lamb.Mengyang Zhang0Peihuang Zhu1Romaric Hippolyte Agassin2Sheng Yao3Dengbao Wang4Zichen Huang5Chi Zhang6Qingqing Hao7Kongshu Ji8State Key Laboratory of Tree Genetics and Breeding, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaState Key Laboratory of Tree Genetics and Breeding, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaState Key Laboratory of Tree Genetics and Breeding, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaState Key Laboratory of Tree Genetics and Breeding, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaState Key Laboratory of Tree Genetics and Breeding, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaState Key Laboratory of Tree Genetics and Breeding, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaState Key Laboratory of Tree Genetics and Breeding, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaState Key Laboratory of Tree Genetics and Breeding, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaState Key Laboratory of Tree Genetics and Breeding, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaBasic leucine zipper (bZIP) transcription factors (TFs) are ubiquitous in eukaryotes. Members of this family play significant roles in the regulation of plant growth, signal transduction, and various stresses. To date, bZIP TFs have been extensively studied in various plants, but there is little information about them in <i>Pinus massoniana</i> Lamb. In this study, 55 bZIP TFs were identified based on data from four different <i>P. massoniana</i> transcriptomes, and a systematic analysis was performed. According to the phylogenetic results, <i>P. massoniana</i> bZIP TFs were divided into 11 groups. Each bZIP protein contained a highly conserved bZIP domain, and the numbers and types of motifs were similar in the same group. The PmbZIPs were nuclear localization proteins. Based on the pine wood nematode inoculation transcriptome, the transcriptional profiles revealed that 25 <i>PmbZIP</i> genes could respond to pine wood nematodes at different levels. Genes <i>PmbZIP3, PmbZIP4, PmbZIP8, PmbZIP20,</i> and <i>PmbZIP23</i> were selected to be upregulated in the process of inoculation with pine wood nematodes. These five genes had different expression levels in different tissues and were responsive to the related treatment conditions. Transcriptional activity analysis showed that PmbZIP3 and PmbZIP8 were transcriptional activators; PmbZIP4, PmbZIP20 and PmbZIP23 were transcriptional repressors. These findings provide preliminary information on PmbZIP TFs, which is helpful for further study of other physiological functions of bZIP TFs in <i>P. massoniana</i>.https://www.mdpi.com/1999-4907/14/1/155<i>Pinus massoniana</i>bZIPstress responsehormoneexpression pattern |
| spellingShingle | Mengyang Zhang Peihuang Zhu Romaric Hippolyte Agassin Sheng Yao Dengbao Wang Zichen Huang Chi Zhang Qingqing Hao Kongshu Ji Identification, Classification and Characterization of bZIP Transcription Factor Family Members in <i>Pinus massoniana</i> Lamb. Forests <i>Pinus massoniana</i> bZIP stress response hormone expression pattern |
| title | Identification, Classification and Characterization of bZIP Transcription Factor Family Members in <i>Pinus massoniana</i> Lamb. |
| title_full | Identification, Classification and Characterization of bZIP Transcription Factor Family Members in <i>Pinus massoniana</i> Lamb. |
| title_fullStr | Identification, Classification and Characterization of bZIP Transcription Factor Family Members in <i>Pinus massoniana</i> Lamb. |
| title_full_unstemmed | Identification, Classification and Characterization of bZIP Transcription Factor Family Members in <i>Pinus massoniana</i> Lamb. |
| title_short | Identification, Classification and Characterization of bZIP Transcription Factor Family Members in <i>Pinus massoniana</i> Lamb. |
| title_sort | identification classification and characterization of bzip transcription factor family members in i pinus massoniana i lamb |
| topic | <i>Pinus massoniana</i> bZIP stress response hormone expression pattern |
| url | https://www.mdpi.com/1999-4907/14/1/155 |
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