Single embryo and oocyte lipid fingerprinting by mass spectrometry[S]
Methods used for lipid analysis in embryos and oocytes usually involve selective lipid extraction from a pool of many samples followed by chemical manipulation, separation and characterization of individual components by chromatographic techniques. Herein we report direct analysis by matrix-assisted...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2010-05-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520410788 |
| _version_ | 1819077223909425152 |
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| author | Christina R. Ferreira Sergio A. Saraiva Rodrigo R. Catharino Jerusa S. Garcia Fabio C. Gozzo Gustavo B. Sanvido Luiz Fernando A. Santos Edson G. Lo Turco José Henrique F. Pontes Andréa C. Basso Ricardo P. Bertolla Roberto Sartori Monique M. Guardieiro Felipe Perecin Flávio V. Meirelles Juliano R. Sangalli Marcos N. Eberlin |
| author_facet | Christina R. Ferreira Sergio A. Saraiva Rodrigo R. Catharino Jerusa S. Garcia Fabio C. Gozzo Gustavo B. Sanvido Luiz Fernando A. Santos Edson G. Lo Turco José Henrique F. Pontes Andréa C. Basso Ricardo P. Bertolla Roberto Sartori Monique M. Guardieiro Felipe Perecin Flávio V. Meirelles Juliano R. Sangalli Marcos N. Eberlin |
| author_sort | Christina R. Ferreira |
| collection | DOAJ |
| description | Methods used for lipid analysis in embryos and oocytes usually involve selective lipid extraction from a pool of many samples followed by chemical manipulation, separation and characterization of individual components by chromatographic techniques. Herein we report direct analysis by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) of single and intact embryos or oocytes from various species. Biological samples were simply moisturized with the matrix solution and characteristic lipid (represented by phosphatidylcholines, sphingomyelins and triacylglycerols) profiles were obtained via MALDI-MS. As representative examples, human, bovine, sheep and fish oocytes, as well as bovine and insect embryos were analyzed. MALDI-MS is shown to be capable of providing characteristic lipid profiles of gametes and embryos and also to respond to modifications due to developmental stages and in vitro culture conditions of bovine embryos. Investigation in developmental biology of the biological roles of structural and reserve lipids in embryos and oocytes should therefore benefit from these rapid MALDI-MS profiles from single and intact species. |
| first_indexed | 2024-12-21T18:53:47Z |
| format | Article |
| id | doaj.art-26d3d721e356461c9ac0635cecb1a92e |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-21T18:53:47Z |
| publishDate | 2010-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-26d3d721e356461c9ac0635cecb1a92e2022-12-21T18:53:41ZengElsevierJournal of Lipid Research0022-22752010-05-0151512181227Single embryo and oocyte lipid fingerprinting by mass spectrometry[S]Christina R. Ferreira0Sergio A. Saraiva1Rodrigo R. Catharino2Jerusa S. Garcia3Fabio C. Gozzo4Gustavo B. Sanvido5Luiz Fernando A. Santos6Edson G. Lo Turco7José Henrique F. Pontes8Andréa C. Basso9Ricardo P. Bertolla10Roberto Sartori11Monique M. Guardieiro12Felipe Perecin13Flávio V. Meirelles14Juliano R. Sangalli15Marcos N. Eberlin16To whom correspondence should be addressed.; Thomson Mass Spectrometry Laboratory, University of Campinas, Campinas, SP, BrazilThomson Mass Spectrometry Laboratory, University of Campinas, Campinas, SP, BrazilDepartment of Clinical Pathology, University of Campinas, Campinas, SP, BrazilThomson Mass Spectrometry Laboratory, University of Campinas, Campinas, SP, BrazilCenter for Structural Molecular Biology, University of Campinas, Campinas, SP, BrazilThomson Mass Spectrometry Laboratory, University of Campinas, Campinas, SP, BrazilCenter for Structural Molecular Biology, University of Campinas, Campinas, SP, BrazilDepartment of Surgery, Federal University of São Paulo, São Paulo, SP, BrazilIn Vitro Brasil Ltda, Mogi Mirim, SP, BrazilIn Vitro Brasil Ltda, Mogi Mirim, SP, BrazilDepartment of Surgery, Federal University of São Paulo, São Paulo, SP, BrazilDepartment of Animal Science, University of São Paulo, Piracicaba, SP, BrazilDepartment of Animal Science, University of São Paulo, Piracicaba, SP, BrazilFaculty of Animal Sciences and Food Engineering, University of São Paulo, Pirassununga, SP, BrazilFaculty of Animal Sciences and Food Engineering, University of São Paulo, Pirassununga, SP, BrazilFaculty of Animal Sciences and Food Engineering, University of São Paulo, Pirassununga, SP, BrazilThomson Mass Spectrometry Laboratory, University of Campinas, Campinas, SP, BrazilMethods used for lipid analysis in embryos and oocytes usually involve selective lipid extraction from a pool of many samples followed by chemical manipulation, separation and characterization of individual components by chromatographic techniques. Herein we report direct analysis by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) of single and intact embryos or oocytes from various species. Biological samples were simply moisturized with the matrix solution and characteristic lipid (represented by phosphatidylcholines, sphingomyelins and triacylglycerols) profiles were obtained via MALDI-MS. As representative examples, human, bovine, sheep and fish oocytes, as well as bovine and insect embryos were analyzed. MALDI-MS is shown to be capable of providing characteristic lipid profiles of gametes and embryos and also to respond to modifications due to developmental stages and in vitro culture conditions of bovine embryos. Investigation in developmental biology of the biological roles of structural and reserve lipids in embryos and oocytes should therefore benefit from these rapid MALDI-MS profiles from single and intact species.http://www.sciencedirect.com/science/article/pii/S0022227520410788oocyteembryomass spectrometryphospholipidtriacylglicerollipid fingerprinting |
| spellingShingle | Christina R. Ferreira Sergio A. Saraiva Rodrigo R. Catharino Jerusa S. Garcia Fabio C. Gozzo Gustavo B. Sanvido Luiz Fernando A. Santos Edson G. Lo Turco José Henrique F. Pontes Andréa C. Basso Ricardo P. Bertolla Roberto Sartori Monique M. Guardieiro Felipe Perecin Flávio V. Meirelles Juliano R. Sangalli Marcos N. Eberlin Single embryo and oocyte lipid fingerprinting by mass spectrometry[S] Journal of Lipid Research oocyte embryo mass spectrometry phospholipid triacylglicerol lipid fingerprinting |
| title | Single embryo and oocyte lipid fingerprinting by mass spectrometry[S] |
| title_full | Single embryo and oocyte lipid fingerprinting by mass spectrometry[S] |
| title_fullStr | Single embryo and oocyte lipid fingerprinting by mass spectrometry[S] |
| title_full_unstemmed | Single embryo and oocyte lipid fingerprinting by mass spectrometry[S] |
| title_short | Single embryo and oocyte lipid fingerprinting by mass spectrometry[S] |
| title_sort | single embryo and oocyte lipid fingerprinting by mass spectrometry s |
| topic | oocyte embryo mass spectrometry phospholipid triacylglicerol lipid fingerprinting |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520410788 |
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