Distribution of salicifoline in freeze-fixed stems of Magnolia kobus as observed by cryo-TOF-SIMS
Abstract Alkaloids are basic nitrogen-containing chemicals that have important physiological and pharmacological characteristics. Many vascular plant species contain alkaloids, and their roles in planta are of interest. However, the detailed distribution of alkaloids remains unclear because of their...
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| Format: | Article |
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Nature Portfolio
2017-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-06444-0 |
| _version_ | 1818752539174109184 |
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| author | Wakaba Okumura Dan Aoki Yasuyuki Matsushita Masato Yoshida Kazuhiko Fukushima |
| author_facet | Wakaba Okumura Dan Aoki Yasuyuki Matsushita Masato Yoshida Kazuhiko Fukushima |
| author_sort | Wakaba Okumura |
| collection | DOAJ |
| description | Abstract Alkaloids are basic nitrogen-containing chemicals that have important physiological and pharmacological characteristics. Many vascular plant species contain alkaloids, and their roles in planta are of interest. However, the detailed distribution of alkaloids remains unclear because of their low water solubility and low concentrations in plants. In this study, we visualized the distribution of salicifoline, a water-soluble quaternary ammonium alkaloid, in the freeze-fixed stems of Magnolia kobus by cryo time-of-flight secondary ion mass spectrometry. Most of the salicifoline was distributed in living phloem tissues. In the xylem, salicifoline was detected in ray cells, lignifying wood fibres, and in vessels in the latest annual ring. The salicifoline distribution in the xylem varied with the cell wall formation stage. These results provide new insights into the storage, transportation, and role of the alkaloid salicifoline in M. kobus. |
| first_indexed | 2024-12-18T04:53:04Z |
| format | Article |
| id | doaj.art-26d43cd941894deb8ec2adb552b2a3ad |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-18T04:53:04Z |
| publishDate | 2017-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-26d43cd941894deb8ec2adb552b2a3ad2022-12-21T21:20:22ZengNature PortfolioScientific Reports2045-23222017-07-01711810.1038/s41598-017-06444-0Distribution of salicifoline in freeze-fixed stems of Magnolia kobus as observed by cryo-TOF-SIMSWakaba Okumura0Dan Aoki1Yasuyuki Matsushita2Masato Yoshida3Kazuhiko Fukushima4Graduate School of Bioagricultural Sciences, Nagoya University, Furo-choGraduate School of Bioagricultural Sciences, Nagoya University, Furo-choGraduate School of Bioagricultural Sciences, Nagoya University, Furo-choGraduate School of Bioagricultural Sciences, Nagoya University, Furo-choGraduate School of Bioagricultural Sciences, Nagoya University, Furo-choAbstract Alkaloids are basic nitrogen-containing chemicals that have important physiological and pharmacological characteristics. Many vascular plant species contain alkaloids, and their roles in planta are of interest. However, the detailed distribution of alkaloids remains unclear because of their low water solubility and low concentrations in plants. In this study, we visualized the distribution of salicifoline, a water-soluble quaternary ammonium alkaloid, in the freeze-fixed stems of Magnolia kobus by cryo time-of-flight secondary ion mass spectrometry. Most of the salicifoline was distributed in living phloem tissues. In the xylem, salicifoline was detected in ray cells, lignifying wood fibres, and in vessels in the latest annual ring. The salicifoline distribution in the xylem varied with the cell wall formation stage. These results provide new insights into the storage, transportation, and role of the alkaloid salicifoline in M. kobus.https://doi.org/10.1038/s41598-017-06444-0 |
| spellingShingle | Wakaba Okumura Dan Aoki Yasuyuki Matsushita Masato Yoshida Kazuhiko Fukushima Distribution of salicifoline in freeze-fixed stems of Magnolia kobus as observed by cryo-TOF-SIMS Scientific Reports |
| title | Distribution of salicifoline in freeze-fixed stems of Magnolia kobus as observed by cryo-TOF-SIMS |
| title_full | Distribution of salicifoline in freeze-fixed stems of Magnolia kobus as observed by cryo-TOF-SIMS |
| title_fullStr | Distribution of salicifoline in freeze-fixed stems of Magnolia kobus as observed by cryo-TOF-SIMS |
| title_full_unstemmed | Distribution of salicifoline in freeze-fixed stems of Magnolia kobus as observed by cryo-TOF-SIMS |
| title_short | Distribution of salicifoline in freeze-fixed stems of Magnolia kobus as observed by cryo-TOF-SIMS |
| title_sort | distribution of salicifoline in freeze fixed stems of magnolia kobus as observed by cryo tof sims |
| url | https://doi.org/10.1038/s41598-017-06444-0 |
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