Expression of Aquaporins in Human Embryos and Potential Role of AQP3 and AQP7 in Preimplantation Mouse Embryo Development
Background/Aims: Water channels, also named aquaporins (AQPs), play crucial roles in cellular water homeostasis. Methods: RT-PCR indicated the mRNA expression of AQPs 1-5, 7, 9, and 11-12, but not AQPs 0, 6, 8, and 10 in the 2∼8-cell stage human embryos. AQP3 and AQP7 were further analyzed for their...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Cell Physiol Biochem Press GmbH & Co KG
2013-05-01
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| Series: | Cellular Physiology and Biochemistry |
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| Online Access: | http://www.karger.com/Article/FullText/350084 |
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| author | Yun Xiong Ya-Jing Tan Yi-Meng Xiong Yi-Ting Huang Xiao-Ling Hu Yong-Chao Lu Ying-Hui Ye Ting-Ting Wang Dan Zhang Fan Jin He-Feng Huang Jian-Zhong Sheng |
| author_facet | Yun Xiong Ya-Jing Tan Yi-Meng Xiong Yi-Ting Huang Xiao-Ling Hu Yong-Chao Lu Ying-Hui Ye Ting-Ting Wang Dan Zhang Fan Jin He-Feng Huang Jian-Zhong Sheng |
| author_sort | Yun Xiong |
| collection | DOAJ |
| description | Background/Aims: Water channels, also named aquaporins (AQPs), play crucial roles in cellular water homeostasis. Methods: RT-PCR indicated the mRNA expression of AQPs 1-5, 7, 9, and 11-12, but not AQPs 0, 6, 8, and 10 in the 2∼8-cell stage human embryos. AQP3 and AQP7 were further analyzed for their mRNA expression and protein expression in the oocyte, zygote, 2-cell embryo, 4-cell embryo, 8-cell embryo, morula, and blastocyst from both human and mouse using RT-PCR and immunofluorescence, respectively. Results: AQP3 and AQP7 were detected in all these stages. Knockdown of either AQP3 or AQP7 by targeted siRNA injection into 2-cell mouse embryos significantly inhibited preimplantation embryo development. However, knockdown of AQP3 in JAr spheroid did not affect its attachment to Ishikawa cells. Conclusion: These data demonstrate that multiple aquaporins are expressed in the early stage human embryos and that AQP3 and AQP7 may play a role in preimplantation mouse embryo development. |
| first_indexed | 2024-12-14T10:43:31Z |
| format | Article |
| id | doaj.art-26d4640a3b394b319a65fcf3c9209a5d |
| institution | Directory Open Access Journal |
| issn | 1015-8987 1421-9778 |
| language | English |
| last_indexed | 2024-12-14T10:43:31Z |
| publishDate | 2013-05-01 |
| publisher | Cell Physiol Biochem Press GmbH & Co KG |
| record_format | Article |
| series | Cellular Physiology and Biochemistry |
| spelling | doaj.art-26d4640a3b394b319a65fcf3c9209a5d2022-12-21T23:05:33ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782013-05-01314-564965810.1159/000350084350084Expression of Aquaporins in Human Embryos and Potential Role of AQP3 and AQP7 in Preimplantation Mouse Embryo DevelopmentYun XiongYa-Jing TanYi-Meng XiongYi-Ting HuangXiao-Ling HuYong-Chao LuYing-Hui YeTing-Ting WangDan ZhangFan JinHe-Feng HuangJian-Zhong ShengBackground/Aims: Water channels, also named aquaporins (AQPs), play crucial roles in cellular water homeostasis. Methods: RT-PCR indicated the mRNA expression of AQPs 1-5, 7, 9, and 11-12, but not AQPs 0, 6, 8, and 10 in the 2∼8-cell stage human embryos. AQP3 and AQP7 were further analyzed for their mRNA expression and protein expression in the oocyte, zygote, 2-cell embryo, 4-cell embryo, 8-cell embryo, morula, and blastocyst from both human and mouse using RT-PCR and immunofluorescence, respectively. Results: AQP3 and AQP7 were detected in all these stages. Knockdown of either AQP3 or AQP7 by targeted siRNA injection into 2-cell mouse embryos significantly inhibited preimplantation embryo development. However, knockdown of AQP3 in JAr spheroid did not affect its attachment to Ishikawa cells. Conclusion: These data demonstrate that multiple aquaporins are expressed in the early stage human embryos and that AQP3 and AQP7 may play a role in preimplantation mouse embryo development.http://www.karger.com/Article/FullText/350084Aquaporin 3Aquaporin 7Early embryoDevelopment |
| spellingShingle | Yun Xiong Ya-Jing Tan Yi-Meng Xiong Yi-Ting Huang Xiao-Ling Hu Yong-Chao Lu Ying-Hui Ye Ting-Ting Wang Dan Zhang Fan Jin He-Feng Huang Jian-Zhong Sheng Expression of Aquaporins in Human Embryos and Potential Role of AQP3 and AQP7 in Preimplantation Mouse Embryo Development Cellular Physiology and Biochemistry Aquaporin 3 Aquaporin 7 Early embryo Development |
| title | Expression of Aquaporins in Human Embryos and Potential Role of AQP3 and AQP7 in Preimplantation Mouse Embryo Development |
| title_full | Expression of Aquaporins in Human Embryos and Potential Role of AQP3 and AQP7 in Preimplantation Mouse Embryo Development |
| title_fullStr | Expression of Aquaporins in Human Embryos and Potential Role of AQP3 and AQP7 in Preimplantation Mouse Embryo Development |
| title_full_unstemmed | Expression of Aquaporins in Human Embryos and Potential Role of AQP3 and AQP7 in Preimplantation Mouse Embryo Development |
| title_short | Expression of Aquaporins in Human Embryos and Potential Role of AQP3 and AQP7 in Preimplantation Mouse Embryo Development |
| title_sort | expression of aquaporins in human embryos and potential role of aqp3 and aqp7 in preimplantation mouse embryo development |
| topic | Aquaporin 3 Aquaporin 7 Early embryo Development |
| url | http://www.karger.com/Article/FullText/350084 |
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