Cold Storage, HepG2, Platelet Transfusion, Trehalose
Objective: Acute myeloid leukemia (AML) is characterized by abnormalities of differentiation and growth of primary hematopoietic stem cells (HSCs) in the blood and bone marrow. In many studies, miR-625-5p has been shown to inhibit downstream pathways from affecting the metastasis and invasion of the...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Royan Institute (ACECR), Tehran
2022-02-01
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| Series: | Cell Journal |
| Subjects: | |
| Online Access: | https://celljournal.org/journal/article/fulltext/upregulation-of-hsa-mir-625-5p-inhibits-invasion-of-acute-myeloid-leukemia-cancer-cells-through-ilkakt-pathway.pdf |
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| author | Sahar Samieyan Dehkordi Seyed Hadi Mousavi Marzieh Ebrahimi Shaban Alizadeh Amir Abbas Hedayati Asl Monireh Mohammad Bahareh Aliabedi |
| author_facet | Sahar Samieyan Dehkordi Seyed Hadi Mousavi Marzieh Ebrahimi Shaban Alizadeh Amir Abbas Hedayati Asl Monireh Mohammad Bahareh Aliabedi |
| author_sort | Sahar Samieyan Dehkordi |
| collection | DOAJ |
| description | Objective: Acute myeloid leukemia (AML) is characterized by abnormalities of differentiation and growth of primary hematopoietic stem cells (HSCs) in the blood and bone marrow. In many studies, miR-625-5p has been shown to inhibit downstream pathways from affecting the metastasis and invasion of the integrin-linked kinase (ILK) signaling pathway. It has been proved that the expression of miR-625-5p decreases in AML cell lines. This study aimed to investigate the effect of miR-625-5p upregulation on the invasion of KG1 ell line in vitro.
Materials and Methods: In this experimental study, we investigated the impact of upregulation of miR-625-5p on invasion via the ILK/AKT pathway in the KG1 cell line. After transfection using the viral method, the cellular invasion was assessed by invasion assay and the levels of miR-625-5p genes and protein were evaluated by quantitative polymerase chain reaction (qPCR) and western blotting. Moreover, CXCR4 level was assessed by flow cytometry.
Results: The invasion significantly reduced in MiR-625-5p-transfected KG1 cells (P<0.01) that was concomitant with remarkably decreasing in the expression levels of ILK, NF-κB, and COX2 genes compare with the control group (P<0.01). Incontrast, MMP9, AP1, and AKT significantly increased (P<0.01, P<0.001 and P<0.01, respectively) and GSK3β did not change significantly in MiR-625-5p-transfected KG1 cells. The protein level of NF-κB decreased (P<0.01) and MMP9 increased, however it was not significant. Moreoever, the expression of CXCR4 was significantly lower (P<0.01) in comparison with the control group.
Conclusion: miR-625-5p leads to a reduction in cell invasion in the AML cell line through ILK pathway. Therefore, it could be a breakthrough in future AML-related research. However, further studies are needed to support this argument. |
| first_indexed | 2024-12-10T20:56:36Z |
| format | Article |
| id | doaj.art-26d4b12f3f934793b5edaf87375c144b |
| institution | Directory Open Access Journal |
| issn | 2228-5806 2228-5814 |
| language | English |
| last_indexed | 2024-12-10T20:56:36Z |
| publishDate | 2022-02-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | Cell Journal |
| spelling | doaj.art-26d4b12f3f934793b5edaf87375c144b2022-12-22T01:33:56ZengRoyan Institute (ACECR), TehranCell Journal2228-58062228-58142022-02-01768410.22074/cellj.2022.7658Cold Storage, HepG2, Platelet Transfusion, TrehaloseSahar Samieyan Dehkordi0Seyed Hadi Mousavi1Marzieh Ebrahimi2Shaban Alizadeh3Amir Abbas Hedayati Asl4Monireh Mohammad5Bahareh Aliabedi6Department of Hematology, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, IranDepartment of Hematology, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, IranDepartment of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, IranDepartment of Hematology, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, IranDepartment of Regenerative Biomedicine, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, IranDepartment of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, IranDepartment of Hematology, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, IranObjective: Acute myeloid leukemia (AML) is characterized by abnormalities of differentiation and growth of primary hematopoietic stem cells (HSCs) in the blood and bone marrow. In many studies, miR-625-5p has been shown to inhibit downstream pathways from affecting the metastasis and invasion of the integrin-linked kinase (ILK) signaling pathway. It has been proved that the expression of miR-625-5p decreases in AML cell lines. This study aimed to investigate the effect of miR-625-5p upregulation on the invasion of KG1 ell line in vitro. Materials and Methods: In this experimental study, we investigated the impact of upregulation of miR-625-5p on invasion via the ILK/AKT pathway in the KG1 cell line. After transfection using the viral method, the cellular invasion was assessed by invasion assay and the levels of miR-625-5p genes and protein were evaluated by quantitative polymerase chain reaction (qPCR) and western blotting. Moreover, CXCR4 level was assessed by flow cytometry. Results: The invasion significantly reduced in MiR-625-5p-transfected KG1 cells (P<0.01) that was concomitant with remarkably decreasing in the expression levels of ILK, NF-κB, and COX2 genes compare with the control group (P<0.01). Incontrast, MMP9, AP1, and AKT significantly increased (P<0.01, P<0.001 and P<0.01, respectively) and GSK3β did not change significantly in MiR-625-5p-transfected KG1 cells. The protein level of NF-κB decreased (P<0.01) and MMP9 increased, however it was not significant. Moreoever, the expression of CXCR4 was significantly lower (P<0.01) in comparison with the control group. Conclusion: miR-625-5p leads to a reduction in cell invasion in the AML cell line through ILK pathway. Therefore, it could be a breakthrough in future AML-related research. However, further studies are needed to support this argument.https://celljournal.org/journal/article/fulltext/upregulation-of-hsa-mir-625-5p-inhibits-invasion-of-acute-myeloid-leukemia-cancer-cells-through-ilkakt-pathway.pdfacute myeloid leukemiacox2integrin-linked kinaseinvasionmmp9 |
| spellingShingle | Sahar Samieyan Dehkordi Seyed Hadi Mousavi Marzieh Ebrahimi Shaban Alizadeh Amir Abbas Hedayati Asl Monireh Mohammad Bahareh Aliabedi Cold Storage, HepG2, Platelet Transfusion, Trehalose Cell Journal acute myeloid leukemia cox2 integrin-linked kinase invasion mmp9 |
| title | Cold Storage, HepG2, Platelet Transfusion, Trehalose |
| title_full | Cold Storage, HepG2, Platelet Transfusion, Trehalose |
| title_fullStr | Cold Storage, HepG2, Platelet Transfusion, Trehalose |
| title_full_unstemmed | Cold Storage, HepG2, Platelet Transfusion, Trehalose |
| title_short | Cold Storage, HepG2, Platelet Transfusion, Trehalose |
| title_sort | cold storage hepg2 platelet transfusion trehalose |
| topic | acute myeloid leukemia cox2 integrin-linked kinase invasion mmp9 |
| url | https://celljournal.org/journal/article/fulltext/upregulation-of-hsa-mir-625-5p-inhibits-invasion-of-acute-myeloid-leukemia-cancer-cells-through-ilkakt-pathway.pdf |
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