Peripheral Nerve-Derived Stem Cell Spheroids Induce Functional Recovery and Repair after Spinal Cord Injury in Rodents
Stem cell therapy is one of the most promising candidate treatments for spinal cord injury. Research has shown optimistic results for this therapy, but clinical limitations remain, including poor viability, engraftment, and differentiation. Here, we isolated novel peripheral nerve-derived stem cells...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/8/4141 |
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| author | Hye-Lan Lee Chung-Eun Yeum HyeYeong Lee Jinsoo Oh Jong-Tae Kim Won-Jin Lee Yoon Ha Young-Il Yang Keung-Nyun Kim |
| author_facet | Hye-Lan Lee Chung-Eun Yeum HyeYeong Lee Jinsoo Oh Jong-Tae Kim Won-Jin Lee Yoon Ha Young-Il Yang Keung-Nyun Kim |
| author_sort | Hye-Lan Lee |
| collection | DOAJ |
| description | Stem cell therapy is one of the most promising candidate treatments for spinal cord injury. Research has shown optimistic results for this therapy, but clinical limitations remain, including poor viability, engraftment, and differentiation. Here, we isolated novel peripheral nerve-derived stem cells (PNSCs) from adult peripheral nerves with similar characteristics to neural-crest stem cells. These PNSCs expressed neural-crest specific markers and showed multilineage differentiation potential into Schwann cells, neuroglia, neurons, and mesodermal cells. In addition, PNSCs showed therapeutic potential by releasing the neurotrophic factors, including glial cell-line-derived neurotrophic factor, insulin-like growth factor, nerve growth factor, and neurotrophin-3. PNSC abilities were also enhanced by their development into spheroids which secreted neurotrophic factors several times more than non-spheroid PNSCs and expressed several types of extra cellular matrix. These features suggest that the potential for these PNSC spheroids can overcome their limitations. In an animal spinal cord injury (SCI) model, these PNSC spheroids induced functional recovery and neuronal regeneration. These PNSC spheroids also reduced the neuropathic pain which accompanies SCI after remyelination. These PNSC spheroids may represent a new therapeutic approach for patients suffering from SCI. |
| first_indexed | 2024-03-10T12:15:09Z |
| format | Article |
| id | doaj.art-26d5e0d6ecdc4c19bafacf4949c233fa |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T12:15:09Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-26d5e0d6ecdc4c19bafacf4949c233fa2023-11-21T15:53:45ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-04-01228414110.3390/ijms22084141Peripheral Nerve-Derived Stem Cell Spheroids Induce Functional Recovery and Repair after Spinal Cord Injury in RodentsHye-Lan Lee0Chung-Eun Yeum1HyeYeong Lee2Jinsoo Oh3Jong-Tae Kim4Won-Jin Lee5Yoon Ha6Young-Il Yang7Keung-Nyun Kim8Spine & Spinal Cord Institute, Department of Neurosurgery, College of Medicine, Yonsei University, Seoul 03722, KoreaPaik Inje Memorial Institute for Clinical Research, Inje University College of Medicine, Busan 47392, KoreaSpine & Spinal Cord Institute, Department of Neurosurgery, College of Medicine, Yonsei University, Seoul 03722, KoreaSpine & Spinal Cord Institute, Department of Neurosurgery, College of Medicine, Yonsei University, Seoul 03722, KoreaPaik Inje Memorial Institute for Clinical Research, Inje University College of Medicine, Busan 47392, KoreaPaik Inje Memorial Institute for Clinical Research, Inje University College of Medicine, Busan 47392, KoreaSpine & Spinal Cord Institute, Department of Neurosurgery, College of Medicine, Yonsei University, Seoul 03722, KoreaPaik Inje Memorial Institute for Clinical Research, Inje University College of Medicine, Busan 47392, KoreaSpine & Spinal Cord Institute, Department of Neurosurgery, College of Medicine, Yonsei University, Seoul 03722, KoreaStem cell therapy is one of the most promising candidate treatments for spinal cord injury. Research has shown optimistic results for this therapy, but clinical limitations remain, including poor viability, engraftment, and differentiation. Here, we isolated novel peripheral nerve-derived stem cells (PNSCs) from adult peripheral nerves with similar characteristics to neural-crest stem cells. These PNSCs expressed neural-crest specific markers and showed multilineage differentiation potential into Schwann cells, neuroglia, neurons, and mesodermal cells. In addition, PNSCs showed therapeutic potential by releasing the neurotrophic factors, including glial cell-line-derived neurotrophic factor, insulin-like growth factor, nerve growth factor, and neurotrophin-3. PNSC abilities were also enhanced by their development into spheroids which secreted neurotrophic factors several times more than non-spheroid PNSCs and expressed several types of extra cellular matrix. These features suggest that the potential for these PNSC spheroids can overcome their limitations. In an animal spinal cord injury (SCI) model, these PNSC spheroids induced functional recovery and neuronal regeneration. These PNSC spheroids also reduced the neuropathic pain which accompanies SCI after remyelination. These PNSC spheroids may represent a new therapeutic approach for patients suffering from SCI.https://www.mdpi.com/1422-0067/22/8/4141peripheral nerve-derived stem cellsspheroidsspinal cord injuryneuroregenerationfunctional recoveryneurotrophic factor |
| spellingShingle | Hye-Lan Lee Chung-Eun Yeum HyeYeong Lee Jinsoo Oh Jong-Tae Kim Won-Jin Lee Yoon Ha Young-Il Yang Keung-Nyun Kim Peripheral Nerve-Derived Stem Cell Spheroids Induce Functional Recovery and Repair after Spinal Cord Injury in Rodents International Journal of Molecular Sciences peripheral nerve-derived stem cells spheroids spinal cord injury neuroregeneration functional recovery neurotrophic factor |
| title | Peripheral Nerve-Derived Stem Cell Spheroids Induce Functional Recovery and Repair after Spinal Cord Injury in Rodents |
| title_full | Peripheral Nerve-Derived Stem Cell Spheroids Induce Functional Recovery and Repair after Spinal Cord Injury in Rodents |
| title_fullStr | Peripheral Nerve-Derived Stem Cell Spheroids Induce Functional Recovery and Repair after Spinal Cord Injury in Rodents |
| title_full_unstemmed | Peripheral Nerve-Derived Stem Cell Spheroids Induce Functional Recovery and Repair after Spinal Cord Injury in Rodents |
| title_short | Peripheral Nerve-Derived Stem Cell Spheroids Induce Functional Recovery and Repair after Spinal Cord Injury in Rodents |
| title_sort | peripheral nerve derived stem cell spheroids induce functional recovery and repair after spinal cord injury in rodents |
| topic | peripheral nerve-derived stem cells spheroids spinal cord injury neuroregeneration functional recovery neurotrophic factor |
| url | https://www.mdpi.com/1422-0067/22/8/4141 |
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