Qualitative and Quantitative Comparison of Plasma Exosomes from Neonates and Adults
Exosomes are extracellular vesicles that contain nucleic acids, lipids and metabolites, and play a critical role in health and disease as mediators of intercellular communication. The majority of extracellular vesicles in the blood are platelet-derived. Compared to adults, neonatal platelets are hyp...
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| Format: | Article |
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MDPI AG
2021-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/4/1926 |
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| author | Julia Peñas-Martínez María N. Barrachina Ernesto José Cuenca-Zamora Ginés Luengo-Gil Susana Belén Bravo Eva Caparrós-Pérez Raúl Teruel-Montoya José Eliseo-Blanco Vicente Vicente Ángel García Irene Martínez-Martínez Francisca Ferrer-Marín |
| author_facet | Julia Peñas-Martínez María N. Barrachina Ernesto José Cuenca-Zamora Ginés Luengo-Gil Susana Belén Bravo Eva Caparrós-Pérez Raúl Teruel-Montoya José Eliseo-Blanco Vicente Vicente Ángel García Irene Martínez-Martínez Francisca Ferrer-Marín |
| author_sort | Julia Peñas-Martínez |
| collection | DOAJ |
| description | Exosomes are extracellular vesicles that contain nucleic acids, lipids and metabolites, and play a critical role in health and disease as mediators of intercellular communication. The majority of extracellular vesicles in the blood are platelet-derived. Compared to adults, neonatal platelets are hyporeactive and show impaired granule release, associated with defects in Soluble N-ethylmaleimide-sensitive fusion Attachment protein REceptor (SNARE) proteins. Since these proteins participate in biogenesis of exosomes, we investigated the potential differences between newborn and adult plasma-derived exosomes. Plasma-derived exosomes were isolated by ultracentrifugation of umbilical cord blood from full-term neonates or peripheral blood from adults. Exosome characterization included size determination by transmission electron microscopy and quantitative proteomic analysis. Plasma-derived exosomes from neonates were significantly smaller and contained 65% less protein than those from adults. Remarkably, 131 proteins were found to be differentially expressed, 83 overexpressed and 48 underexpressed in neonatal (vs. adult) exosomes. Whereas the upregulated proteins in plasma exosomes from neonates are associated with platelet activation, coagulation and granule secretion, most of the underexpressed proteins are immunoglobulins. This is the first study showing that exosome size and content change with age. Our findings may contribute to elucidating the potential “developmental hemostatic mismatch risk” associated with transfusions containing plasma exosomes from adults. |
| first_indexed | 2024-03-09T00:51:15Z |
| format | Article |
| id | doaj.art-26d7807a6c9c4b95a5db83b799d1880b |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T00:51:15Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-26d7807a6c9c4b95a5db83b799d1880b2023-12-11T17:11:31ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-02-01224192610.3390/ijms22041926Qualitative and Quantitative Comparison of Plasma Exosomes from Neonates and AdultsJulia Peñas-Martínez0María N. Barrachina1Ernesto José Cuenca-Zamora2Ginés Luengo-Gil3Susana Belén Bravo4Eva Caparrós-Pérez5Raúl Teruel-Montoya6José Eliseo-Blanco7Vicente Vicente8Ángel García9Irene Martínez-Martínez10Francisca Ferrer-Marín11Servicio de Hematología y Oncología Médica, Hospital Universitario Morales Meseguer, Centro Regional de Hemodonación, Universidad de Murcia, IMIB-Arrixaca, 30003 Murcia, SpainPlatelet Proteomics Group, Centro Singular de Investigación en Medicina Molecular y Enfermedades Crónicas (CIMUS), Universidad de Santiago de Compostela e Instituto de Investigación Sanitaria de Santiago (IDIS), 15706 Santiago de Compostela, SpainServicio de Hematología y Oncología Médica, Hospital Universitario Morales Meseguer, Centro Regional de Hemodonación, Universidad de Murcia, IMIB-Arrixaca, 30003 Murcia, SpainServicio de Hematología y Oncología Médica, Hospital Universitario Morales Meseguer, Centro Regional de Hemodonación, Universidad de Murcia, IMIB-Arrixaca, 30003 Murcia, SpainServicio de Proteomica, e Instituto de Investigación Sanitaria de Santiago de Compostela (IDIS), Hospital ClínicoUniversitario de Santiago de Compostela, 15706 Santiago de Compostela, SpainServicio de Hematología y Oncología Médica, Hospital Universitario Morales Meseguer, Centro Regional de Hemodonación, Universidad de Murcia, IMIB-Arrixaca, 30003 Murcia, SpainServicio de Hematología y Oncología Médica, Hospital Universitario Morales Meseguer, Centro Regional de Hemodonación, Universidad de Murcia, IMIB-Arrixaca, 30003 Murcia, SpainServicio de Obstetricia y Ginecología, Hospital Clínico Virgen de la Arrixaca, 30120 Murcia, SpainServicio de Hematología y Oncología Médica, Hospital Universitario Morales Meseguer, Centro Regional de Hemodonación, Universidad de Murcia, IMIB-Arrixaca, 30003 Murcia, SpainPlatelet Proteomics Group, Centro Singular de Investigación en Medicina Molecular y Enfermedades Crónicas (CIMUS), Universidad de Santiago de Compostela e Instituto de Investigación Sanitaria de Santiago (IDIS), 15706 Santiago de Compostela, SpainServicio de Hematología y Oncología Médica, Hospital Universitario Morales Meseguer, Centro Regional de Hemodonación, Universidad de Murcia, IMIB-Arrixaca, 30003 Murcia, SpainServicio de Hematología y Oncología Médica, Hospital Universitario Morales Meseguer, Centro Regional de Hemodonación, Universidad de Murcia, IMIB-Arrixaca, 30003 Murcia, SpainExosomes are extracellular vesicles that contain nucleic acids, lipids and metabolites, and play a critical role in health and disease as mediators of intercellular communication. The majority of extracellular vesicles in the blood are platelet-derived. Compared to adults, neonatal platelets are hyporeactive and show impaired granule release, associated with defects in Soluble N-ethylmaleimide-sensitive fusion Attachment protein REceptor (SNARE) proteins. Since these proteins participate in biogenesis of exosomes, we investigated the potential differences between newborn and adult plasma-derived exosomes. Plasma-derived exosomes were isolated by ultracentrifugation of umbilical cord blood from full-term neonates or peripheral blood from adults. Exosome characterization included size determination by transmission electron microscopy and quantitative proteomic analysis. Plasma-derived exosomes from neonates were significantly smaller and contained 65% less protein than those from adults. Remarkably, 131 proteins were found to be differentially expressed, 83 overexpressed and 48 underexpressed in neonatal (vs. adult) exosomes. Whereas the upregulated proteins in plasma exosomes from neonates are associated with platelet activation, coagulation and granule secretion, most of the underexpressed proteins are immunoglobulins. This is the first study showing that exosome size and content change with age. Our findings may contribute to elucidating the potential “developmental hemostatic mismatch risk” associated with transfusions containing plasma exosomes from adults.https://www.mdpi.com/1422-0067/22/4/1926exosomesneonatal plateletsplatelet transfusionprotein Sproteomicvon Willebrand factor |
| spellingShingle | Julia Peñas-Martínez María N. Barrachina Ernesto José Cuenca-Zamora Ginés Luengo-Gil Susana Belén Bravo Eva Caparrós-Pérez Raúl Teruel-Montoya José Eliseo-Blanco Vicente Vicente Ángel García Irene Martínez-Martínez Francisca Ferrer-Marín Qualitative and Quantitative Comparison of Plasma Exosomes from Neonates and Adults International Journal of Molecular Sciences exosomes neonatal platelets platelet transfusion protein S proteomic von Willebrand factor |
| title | Qualitative and Quantitative Comparison of Plasma Exosomes from Neonates and Adults |
| title_full | Qualitative and Quantitative Comparison of Plasma Exosomes from Neonates and Adults |
| title_fullStr | Qualitative and Quantitative Comparison of Plasma Exosomes from Neonates and Adults |
| title_full_unstemmed | Qualitative and Quantitative Comparison of Plasma Exosomes from Neonates and Adults |
| title_short | Qualitative and Quantitative Comparison of Plasma Exosomes from Neonates and Adults |
| title_sort | qualitative and quantitative comparison of plasma exosomes from neonates and adults |
| topic | exosomes neonatal platelets platelet transfusion protein S proteomic von Willebrand factor |
| url | https://www.mdpi.com/1422-0067/22/4/1926 |
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