Human Lactobacillus Strains from the Intestine can Suppress IgE-Mediated Degranulation of Rat Basophilic Leukaemia (RBL-2H3) Cells
Mast cells play a critical role in immunoglobulin E (IgE)-mediated allergic diseases, and the degranulation of mast cells is important in the pathogenesis of these diseases. A disturbance of the intestinal microflora, especially of endogenous lactic acid bacteria, might be a contributing factor for...
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MDPI AG
2016-10-01
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| Series: | Microorganisms |
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| Online Access: | http://www.mdpi.com/2076-2607/4/4/40 |
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| author | Gaku Harata Fang He Kyoko Takahashi Akira Hosono Kenji Miyazawa Kazutoyo Yoda Masaru Hiramatsu Shuichi Kaminogawa |
| author_facet | Gaku Harata Fang He Kyoko Takahashi Akira Hosono Kenji Miyazawa Kazutoyo Yoda Masaru Hiramatsu Shuichi Kaminogawa |
| author_sort | Gaku Harata |
| collection | DOAJ |
| description | Mast cells play a critical role in immunoglobulin E (IgE)-mediated allergic diseases, and the degranulation of mast cells is important in the pathogenesis of these diseases. A disturbance of the intestinal microflora, especially of endogenous lactic acid bacteria, might be a contributing factor for IgE-mediated allergic diseases. Additional knowledge regarding the interaction of human intestinal Lactobacilli with mast cells is still necessary. Twenty-three strains of Lactobacilli, including commercial and reference strains and strains from the human intestine, were tested for their ability to regulate degranulation of cells from rat basophilic leukemia RBL-2H3 cells (RBL-2H3) in vitro based on a β-hexosaminidase release assay. Each of the tested Lactobacilli characteristically suppressed IgE-mediated degranulation of RBL-2H3 cells, and Lactobacillus GG showed the strongest inhibitory effect on the cells. Furthermore, the bacteria isolated from the human intestine significantly suppressed degranulation of RBL-2H3 cellsin comparison with the reference strains. These results suggest that Lactobacilli, particularly those from the human intestine, can affect the activation of mast cells in a strain-dependent manner. Further study should be conducted to analyse the understanding mechanism. |
| first_indexed | 2024-12-10T13:30:04Z |
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| id | doaj.art-26d854d4a30c47ebb2bb3c17551c28d7 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-12-10T13:30:04Z |
| publishDate | 2016-10-01 |
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| series | Microorganisms |
| spelling | doaj.art-26d854d4a30c47ebb2bb3c17551c28d72022-12-22T01:47:01ZengMDPI AGMicroorganisms2076-26072016-10-01444010.3390/microorganisms4040040microorganisms4040040Human Lactobacillus Strains from the Intestine can Suppress IgE-Mediated Degranulation of Rat Basophilic Leukaemia (RBL-2H3) CellsGaku Harata0Fang He1Kyoko Takahashi2Akira Hosono3Kenji Miyazawa4Kazutoyo Yoda5Masaru Hiramatsu6Shuichi Kaminogawa7Technical Research Laboratory, Takanashi Milk Products Co., Ltd., Yokohama 241-0023, JapanTechnical Research Laboratory, Takanashi Milk Products Co., Ltd., Yokohama 241-0023, JapanDepartment of Food Bioscience and Biotechnology, College of Bioresource Sciences, Nihon University, Fujisawa 252-8510, JapanDepartment of Food Bioscience and Biotechnology, College of Bioresource Sciences, Nihon University, Fujisawa 252-8510, JapanTechnical Research Laboratory, Takanashi Milk Products Co., Ltd., Yokohama 241-0023, JapanTechnical Research Laboratory, Takanashi Milk Products Co., Ltd., Yokohama 241-0023, JapanTechnical Research Laboratory, Takanashi Milk Products Co., Ltd., Yokohama 241-0023, JapanDepartment of Food Bioscience and Biotechnology, College of Bioresource Sciences, Nihon University, Fujisawa 252-8510, JapanMast cells play a critical role in immunoglobulin E (IgE)-mediated allergic diseases, and the degranulation of mast cells is important in the pathogenesis of these diseases. A disturbance of the intestinal microflora, especially of endogenous lactic acid bacteria, might be a contributing factor for IgE-mediated allergic diseases. Additional knowledge regarding the interaction of human intestinal Lactobacilli with mast cells is still necessary. Twenty-three strains of Lactobacilli, including commercial and reference strains and strains from the human intestine, were tested for their ability to regulate degranulation of cells from rat basophilic leukemia RBL-2H3 cells (RBL-2H3) in vitro based on a β-hexosaminidase release assay. Each of the tested Lactobacilli characteristically suppressed IgE-mediated degranulation of RBL-2H3 cells, and Lactobacillus GG showed the strongest inhibitory effect on the cells. Furthermore, the bacteria isolated from the human intestine significantly suppressed degranulation of RBL-2H3 cellsin comparison with the reference strains. These results suggest that Lactobacilli, particularly those from the human intestine, can affect the activation of mast cells in a strain-dependent manner. Further study should be conducted to analyse the understanding mechanism.http://www.mdpi.com/2076-2607/4/4/40degranulationIgE-mediated allergyLactobacillimast cell |
| spellingShingle | Gaku Harata Fang He Kyoko Takahashi Akira Hosono Kenji Miyazawa Kazutoyo Yoda Masaru Hiramatsu Shuichi Kaminogawa Human Lactobacillus Strains from the Intestine can Suppress IgE-Mediated Degranulation of Rat Basophilic Leukaemia (RBL-2H3) Cells Microorganisms degranulation IgE-mediated allergy Lactobacilli mast cell |
| title | Human Lactobacillus Strains from the Intestine can Suppress IgE-Mediated Degranulation of Rat Basophilic Leukaemia (RBL-2H3) Cells |
| title_full | Human Lactobacillus Strains from the Intestine can Suppress IgE-Mediated Degranulation of Rat Basophilic Leukaemia (RBL-2H3) Cells |
| title_fullStr | Human Lactobacillus Strains from the Intestine can Suppress IgE-Mediated Degranulation of Rat Basophilic Leukaemia (RBL-2H3) Cells |
| title_full_unstemmed | Human Lactobacillus Strains from the Intestine can Suppress IgE-Mediated Degranulation of Rat Basophilic Leukaemia (RBL-2H3) Cells |
| title_short | Human Lactobacillus Strains from the Intestine can Suppress IgE-Mediated Degranulation of Rat Basophilic Leukaemia (RBL-2H3) Cells |
| title_sort | human lactobacillus strains from the intestine can suppress ige mediated degranulation of rat basophilic leukaemia rbl 2h3 cells |
| topic | degranulation IgE-mediated allergy Lactobacilli mast cell |
| url | http://www.mdpi.com/2076-2607/4/4/40 |
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