Protective effect of polysaccharide from the artificially cultivated Sanghuangporus vaninii against H2O2-induced toxicity in vitro and in zebrafish models
PFSV-2 is a purified polysaccharide with good hypolipidemic activity from artificially cultivated Sanghuangporus vaninii. In this study, PFSV-2 was characterized by scanning electron microscopy, atomic force microscopy, X-ray diffraction, zeta potential, thermogravimetric analysis and the Congo red...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-10-01
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| Series: | Arabian Journal of Chemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1878535223005774 |
| _version_ | 1827823048802172928 |
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| author | Zhang Zuofa Lv Guoying Shen Meng Song Tingting Peng Juan |
| author_facet | Zhang Zuofa Lv Guoying Shen Meng Song Tingting Peng Juan |
| author_sort | Zhang Zuofa |
| collection | DOAJ |
| description | PFSV-2 is a purified polysaccharide with good hypolipidemic activity from artificially cultivated Sanghuangporus vaninii. In this study, PFSV-2 was characterized by scanning electron microscopy, atomic force microscopy, X-ray diffraction, zeta potential, thermogravimetric analysis and the Congo red test. To assess the protective effect of PFSV-2, H2O2-induced oxidant injury on L929 cells and larval zebrafish models was employed. PFSV-2 significantly reduced H2O2-induced cytotoxicity in L929 cells by decreasing intracellular ROS levels and cell apoptosis. Furthermore, PFSV-2 had an obvious protective effect against H2O2-stimulated oxidative stress in larval zebrafish by decreasing heart rate and reducing ROS generation and cells death. Additionally, PFSV-2 decreased the production of MDA and increased the activity of antioxidant enzymes SOD and CAT in both L929 cells and larval zebrafish. These results suggested that PFSV-2 may be useful as an antioxidant in the medical and cosmetic industries. |
| first_indexed | 2024-03-12T02:05:51Z |
| format | Article |
| id | doaj.art-26d8b8edd3184adda2e7489080758bf8 |
| institution | Directory Open Access Journal |
| issn | 1878-5352 |
| language | English |
| last_indexed | 2024-03-12T02:05:51Z |
| publishDate | 2023-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Arabian Journal of Chemistry |
| spelling | doaj.art-26d8b8edd3184adda2e7489080758bf82023-09-07T04:43:27ZengElsevierArabian Journal of Chemistry1878-53522023-10-011610105115Protective effect of polysaccharide from the artificially cultivated Sanghuangporus vaninii against H2O2-induced toxicity in vitro and in zebrafish modelsZhang Zuofa0Lv Guoying1Shen Meng2Song Tingting3Peng Juan4Institute of Horticulture, Zhejiang Academy of Agricultural Science, Hangzhou 310021, China; Corresponding author.Institute of Horticulture, Zhejiang Academy of Agricultural Science, Hangzhou 310021, ChinaJiaxing Academy of Agricultural Science, Jiaxing 314000, ChinaInstitute of Horticulture, Zhejiang Academy of Agricultural Science, Hangzhou 310021, ChinaInstitute of Horticulture, Zhejiang Academy of Agricultural Science, Hangzhou 310021, ChinaPFSV-2 is a purified polysaccharide with good hypolipidemic activity from artificially cultivated Sanghuangporus vaninii. In this study, PFSV-2 was characterized by scanning electron microscopy, atomic force microscopy, X-ray diffraction, zeta potential, thermogravimetric analysis and the Congo red test. To assess the protective effect of PFSV-2, H2O2-induced oxidant injury on L929 cells and larval zebrafish models was employed. PFSV-2 significantly reduced H2O2-induced cytotoxicity in L929 cells by decreasing intracellular ROS levels and cell apoptosis. Furthermore, PFSV-2 had an obvious protective effect against H2O2-stimulated oxidative stress in larval zebrafish by decreasing heart rate and reducing ROS generation and cells death. Additionally, PFSV-2 decreased the production of MDA and increased the activity of antioxidant enzymes SOD and CAT in both L929 cells and larval zebrafish. These results suggested that PFSV-2 may be useful as an antioxidant in the medical and cosmetic industries.http://www.sciencedirect.com/science/article/pii/S1878535223005774Sanghuangporus vaniniiPurified polysaccharideAntioxidant activityZebrafishL929 cells |
| spellingShingle | Zhang Zuofa Lv Guoying Shen Meng Song Tingting Peng Juan Protective effect of polysaccharide from the artificially cultivated Sanghuangporus vaninii against H2O2-induced toxicity in vitro and in zebrafish models Arabian Journal of Chemistry Sanghuangporus vaninii Purified polysaccharide Antioxidant activity Zebrafish L929 cells |
| title | Protective effect of polysaccharide from the artificially cultivated Sanghuangporus vaninii against H2O2-induced toxicity in vitro and in zebrafish models |
| title_full | Protective effect of polysaccharide from the artificially cultivated Sanghuangporus vaninii against H2O2-induced toxicity in vitro and in zebrafish models |
| title_fullStr | Protective effect of polysaccharide from the artificially cultivated Sanghuangporus vaninii against H2O2-induced toxicity in vitro and in zebrafish models |
| title_full_unstemmed | Protective effect of polysaccharide from the artificially cultivated Sanghuangporus vaninii against H2O2-induced toxicity in vitro and in zebrafish models |
| title_short | Protective effect of polysaccharide from the artificially cultivated Sanghuangporus vaninii against H2O2-induced toxicity in vitro and in zebrafish models |
| title_sort | protective effect of polysaccharide from the artificially cultivated sanghuangporus vaninii against h2o2 induced toxicity in vitro and in zebrafish models |
| topic | Sanghuangporus vaninii Purified polysaccharide Antioxidant activity Zebrafish L929 cells |
| url | http://www.sciencedirect.com/science/article/pii/S1878535223005774 |
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