Xylanase Production by <i>Talaromyces amestolkiae</i> Valuing Agroindustrial Byproducts
In general, agroindustrial byproducts can be easily assimilated by several microorganisms due to their composition, which is rich in carbohydrates. Therefore, they could be appropriate for use as raw materials in a sustainable refinery concept, including the production of hydrolytic enzymes with ind...
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MDPI AG
2022-05-01
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| Series: | BioTech |
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| Online Access: | https://www.mdpi.com/2673-6284/11/2/15 |
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| author | Giórgia S. Barbieri Heitor B. S. Bento Fernanda de Oliveira Flávio P. Picheli Lídia M. Dias Fernando Masarin Valéria C. Santos-Ebinuma |
| author_facet | Giórgia S. Barbieri Heitor B. S. Bento Fernanda de Oliveira Flávio P. Picheli Lídia M. Dias Fernando Masarin Valéria C. Santos-Ebinuma |
| author_sort | Giórgia S. Barbieri |
| collection | DOAJ |
| description | In general, agroindustrial byproducts can be easily assimilated by several microorganisms due to their composition, which is rich in carbohydrates. Therefore, they could be appropriate for use as raw materials in a sustainable refinery concept, including the production of hydrolytic enzymes with industrial applicability. In this work, xylanase production by the filamentous fungi <i>Talaromyces amestolkiae</i> in submerged culture was evaluated using five agroindustrial byproducts, namely, wheat bran, citrus pulp, rice bran, peanut skin, and peanut shell. Firstly, the aforementioned byproducts were characterized in terms of cellulose, xylan, lignin, and extractives. Next, production studies were performed, and wheat bran generated the highest enzymatic activity (5.4 U·mL<sup>−1</sup>), probably because of its large amount of xylan. Subsequently, a factorial design was performed to evaluate the independent variables yeast extract, wheat bran, K<sub>2</sub>HPO<sub>4</sub>, and pH, aiming to improve the variable response, xylanase activity. The condition that promoted the highest production, 13.02 U·mL<sup>−1</sup> (141% higher than the initial condition), was 20 g·L<sup>−1</sup> wheat bran, 2.5 g·L<sup>−1</sup> yeast extract, 3 g·L<sup>−1</sup> K<sub>2</sub>HPO<sub>4</sub>, and pH 7. Thus, industrial byproducts with a high content of xylan can be used as a culture medium to produce xylanase enzymes with a <i>Talaromyces</i> strain through an economical and sustainable approach. |
| first_indexed | 2024-03-10T00:16:41Z |
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| language | English |
| last_indexed | 2024-03-10T00:16:41Z |
| publishDate | 2022-05-01 |
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| record_format | Article |
| series | BioTech |
| spelling | doaj.art-26e142b8d43b46328a71a710c7f9c97a2023-11-23T15:49:37ZengMDPI AGBioTech2673-62842022-05-011121510.3390/biotech11020015Xylanase Production by <i>Talaromyces amestolkiae</i> Valuing Agroindustrial ByproductsGiórgia S. Barbieri0Heitor B. S. Bento1Fernanda de Oliveira2Flávio P. Picheli3Lídia M. Dias4Fernando Masarin5Valéria C. Santos-Ebinuma6Department of Engineering Bioprocess and Biotechnology, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Rodovia Araraquara-Jau km. 01, Araraquara 14800-903, SP, BrazilDepartment of Engineering Bioprocess and Biotechnology, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Rodovia Araraquara-Jau km. 01, Araraquara 14800-903, SP, BrazilDepartment of Engineering Bioprocess and Biotechnology, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Rodovia Araraquara-Jau km. 01, Araraquara 14800-903, SP, BrazilDepartment of Engineering Bioprocess and Biotechnology, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Rodovia Araraquara-Jau km. 01, Araraquara 14800-903, SP, BrazilDepartment of Engineering Bioprocess and Biotechnology, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Rodovia Araraquara-Jau km. 01, Araraquara 14800-903, SP, BrazilDepartment of Engineering Bioprocess and Biotechnology, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Rodovia Araraquara-Jau km. 01, Araraquara 14800-903, SP, BrazilDepartment of Engineering Bioprocess and Biotechnology, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Rodovia Araraquara-Jau km. 01, Araraquara 14800-903, SP, BrazilIn general, agroindustrial byproducts can be easily assimilated by several microorganisms due to their composition, which is rich in carbohydrates. Therefore, they could be appropriate for use as raw materials in a sustainable refinery concept, including the production of hydrolytic enzymes with industrial applicability. In this work, xylanase production by the filamentous fungi <i>Talaromyces amestolkiae</i> in submerged culture was evaluated using five agroindustrial byproducts, namely, wheat bran, citrus pulp, rice bran, peanut skin, and peanut shell. Firstly, the aforementioned byproducts were characterized in terms of cellulose, xylan, lignin, and extractives. Next, production studies were performed, and wheat bran generated the highest enzymatic activity (5.4 U·mL<sup>−1</sup>), probably because of its large amount of xylan. Subsequently, a factorial design was performed to evaluate the independent variables yeast extract, wheat bran, K<sub>2</sub>HPO<sub>4</sub>, and pH, aiming to improve the variable response, xylanase activity. The condition that promoted the highest production, 13.02 U·mL<sup>−1</sup> (141% higher than the initial condition), was 20 g·L<sup>−1</sup> wheat bran, 2.5 g·L<sup>−1</sup> yeast extract, 3 g·L<sup>−1</sup> K<sub>2</sub>HPO<sub>4</sub>, and pH 7. Thus, industrial byproducts with a high content of xylan can be used as a culture medium to produce xylanase enzymes with a <i>Talaromyces</i> strain through an economical and sustainable approach.https://www.mdpi.com/2673-6284/11/2/15xylanase<i>Talaromyces amestolkiae</i>enzymatic hydrolysissubmerged cultivationagroindustrial byproducts |
| spellingShingle | Giórgia S. Barbieri Heitor B. S. Bento Fernanda de Oliveira Flávio P. Picheli Lídia M. Dias Fernando Masarin Valéria C. Santos-Ebinuma Xylanase Production by <i>Talaromyces amestolkiae</i> Valuing Agroindustrial Byproducts BioTech xylanase <i>Talaromyces amestolkiae</i> enzymatic hydrolysis submerged cultivation agroindustrial byproducts |
| title | Xylanase Production by <i>Talaromyces amestolkiae</i> Valuing Agroindustrial Byproducts |
| title_full | Xylanase Production by <i>Talaromyces amestolkiae</i> Valuing Agroindustrial Byproducts |
| title_fullStr | Xylanase Production by <i>Talaromyces amestolkiae</i> Valuing Agroindustrial Byproducts |
| title_full_unstemmed | Xylanase Production by <i>Talaromyces amestolkiae</i> Valuing Agroindustrial Byproducts |
| title_short | Xylanase Production by <i>Talaromyces amestolkiae</i> Valuing Agroindustrial Byproducts |
| title_sort | xylanase production by i talaromyces amestolkiae i valuing agroindustrial byproducts |
| topic | xylanase <i>Talaromyces amestolkiae</i> enzymatic hydrolysis submerged cultivation agroindustrial byproducts |
| url | https://www.mdpi.com/2673-6284/11/2/15 |
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