Extended spectrum beta-lactamases in <i>Escherichia coli</i> from municipal wastewater
Introduction and objective Over the past decades, awareness of the environmental load of resistant organisms has increased. The presented paper focuses on antibiotic resistance and detection of resistance genes in environmental <i>E. coli</i> and on the evaluation of biofilm formation in...
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| Format: | Article |
| Language: | English |
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Institute of Rural Health
2015-09-01
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| Series: | Annals of Agricultural and Environmental Medicine |
| Subjects: | |
| Online Access: | http://www.journalssystem.com/aaem/Extended-spectrum-beta-lactamases-in-Escherichia-coli-from-municipal-wastewater,72307,0,2.html |
| _version_ | 1818694970007093248 |
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| author | Tatiana Čornejová Jan Venglovsky Gabriela Gregova Marta Kmetova Vladimir Kmet |
| author_facet | Tatiana Čornejová Jan Venglovsky Gabriela Gregova Marta Kmetova Vladimir Kmet |
| author_sort | Tatiana Čornejová |
| collection | DOAJ |
| description | Introduction and objective
Over the past decades, awareness of the environmental load of resistant organisms has increased. The presented paper focuses on antibiotic resistance and detection of resistance genes in environmental <i>E. coli</i> and on the evaluation of biofilm formation in ESBLs (extended spectrum beta-lactamase) producing <i>E. coli</i> isolated from an urban wastewater treatment plant.
Material and Methods
Wastewater samples and artificially added polystyrene pellets were used as the source for <i>E. coli</i> isolation. Minimal inhibitory concentrations of 19 antibiotics were determined according to CLSI (2013). Biofilm formation was investigated by crystal violet or resazurin methods. CTX-M, carbapenemases, <i>qnrS</i>, mobile elements and virulence factors were determined by PCR. Clonal relatedness of strains was detected by principal component analysis by a Maldi biotyper.
Results
ESBL phenotype was detected in 26% of environmental strains. CTX-M, CMY-2 and <i>qnrS</i> genes of antibiotic resistance were detected. IMP gene together with integron 1 in one ertapenem resistant <i>E. coli</i> was also recorded. There was no evident correlation between antibiotic resistance, virulence and biofilm production.
Conclusions
Conclusions. The results showed that the wastewater is a source of ESBLs, carbapenemases and plasmid fluoroquinolone resistance. Strains with biofilm production, antibiotic resistance of CTX-M group, CMY-2, <i>qnrS</i> genes and virulence factors present a potential environmental health risk. |
| first_indexed | 2024-12-17T13:38:02Z |
| format | Article |
| id | doaj.art-26e1a44931564d648995e2eb6a9301a5 |
| institution | Directory Open Access Journal |
| issn | 1232-1966 1898-2263 |
| language | English |
| last_indexed | 2024-12-17T13:38:02Z |
| publishDate | 2015-09-01 |
| publisher | Institute of Rural Health |
| record_format | Article |
| series | Annals of Agricultural and Environmental Medicine |
| spelling | doaj.art-26e1a44931564d648995e2eb6a9301a52022-12-21T21:46:23ZengInstitute of Rural HealthAnnals of Agricultural and Environmental Medicine1232-19661898-22632015-09-0122344745010.5604/12321966.116771072307Extended spectrum beta-lactamases in <i>Escherichia coli</i> from municipal wastewaterTatiana Čornejová0Jan Venglovsky1Gabriela Gregova2Marta Kmetova3Vladimir Kmet4University of Veterinary Medicine and Pharmacy, Košice, SlovakiaUniversity of Veterinary Medicine and Pharmacy, Košice, SlovakiaUniversity of Veterinary Medicine and Pharmacy, Košice, SlovakiaFaculty of Medicine, Department of Medical Microbiology and Clinical Microbiology, P. J. Safarik University, Kosice, SlovakiaInstitute of Animal Physiology, Slovak Academy of Sciences, Košice, SlovakiaIntroduction and objective Over the past decades, awareness of the environmental load of resistant organisms has increased. The presented paper focuses on antibiotic resistance and detection of resistance genes in environmental <i>E. coli</i> and on the evaluation of biofilm formation in ESBLs (extended spectrum beta-lactamase) producing <i>E. coli</i> isolated from an urban wastewater treatment plant. Material and Methods Wastewater samples and artificially added polystyrene pellets were used as the source for <i>E. coli</i> isolation. Minimal inhibitory concentrations of 19 antibiotics were determined according to CLSI (2013). Biofilm formation was investigated by crystal violet or resazurin methods. CTX-M, carbapenemases, <i>qnrS</i>, mobile elements and virulence factors were determined by PCR. Clonal relatedness of strains was detected by principal component analysis by a Maldi biotyper. Results ESBL phenotype was detected in 26% of environmental strains. CTX-M, CMY-2 and <i>qnrS</i> genes of antibiotic resistance were detected. IMP gene together with integron 1 in one ertapenem resistant <i>E. coli</i> was also recorded. There was no evident correlation between antibiotic resistance, virulence and biofilm production. Conclusions Conclusions. The results showed that the wastewater is a source of ESBLs, carbapenemases and plasmid fluoroquinolone resistance. Strains with biofilm production, antibiotic resistance of CTX-M group, CMY-2, <i>qnrS</i> genes and virulence factors present a potential environmental health risk.http://www.journalssystem.com/aaem/Extended-spectrum-beta-lactamases-in-Escherichia-coli-from-municipal-wastewater,72307,0,2.htmlE.coliESBLvirulencebiofilm |
| spellingShingle | Tatiana Čornejová Jan Venglovsky Gabriela Gregova Marta Kmetova Vladimir Kmet Extended spectrum beta-lactamases in <i>Escherichia coli</i> from municipal wastewater Annals of Agricultural and Environmental Medicine E.coli ESBL virulence biofilm |
| title | Extended spectrum beta-lactamases in <i>Escherichia coli</i> from municipal wastewater |
| title_full | Extended spectrum beta-lactamases in <i>Escherichia coli</i> from municipal wastewater |
| title_fullStr | Extended spectrum beta-lactamases in <i>Escherichia coli</i> from municipal wastewater |
| title_full_unstemmed | Extended spectrum beta-lactamases in <i>Escherichia coli</i> from municipal wastewater |
| title_short | Extended spectrum beta-lactamases in <i>Escherichia coli</i> from municipal wastewater |
| title_sort | extended spectrum beta lactamases in i escherichia coli i from municipal wastewater |
| topic | E.coli ESBL virulence biofilm |
| url | http://www.journalssystem.com/aaem/Extended-spectrum-beta-lactamases-in-Escherichia-coli-from-municipal-wastewater,72307,0,2.html |
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