The study of UCH-L1 and S100B serum concentration in the diagnosis of diffuse and focal severe traumatic brain injury
Purpose: To evaluate the feasibility of determining serum concentration of biomarker of neuronal protein damage UCH-L1 and biomarker of astroglia damage S100 on the 1st day after injury, particularly for the diagnosis of focal and diffuse brain impairments based on clinical and biochemica...
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| Format: | Article |
| Language: | English |
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Romodanov Neurosurgery Institute
2017-12-01
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| Series: | Ukrainian Neurosurgical Journal |
| Online Access: | https://theunj.org/article/view/113373 |
| _version_ | 1827377571224879104 |
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| author | Oleg Y. Kobyletsky Lyudmyla M. Bielska Volodymyr M. Shevaha Vadym V. Biloshytsky |
| author_facet | Oleg Y. Kobyletsky Lyudmyla M. Bielska Volodymyr M. Shevaha Vadym V. Biloshytsky |
| author_sort | Oleg Y. Kobyletsky |
| collection | DOAJ |
| description |
Purpose: To evaluate the feasibility of determining serum concentration of biomarker of neuronal protein damage UCH-L1 and biomarker of astroglia damage S100 on the 1st day after injury, particularly for the diagnosis of focal and diffuse brain impairments based on clinical and biochemical and computer tomographic study in patients with acute severe brain injury.Methods. We used the results of diagnostic tests and therapeutic manipulations in 72 patients aged 16 to 76 years with severe traumatic brain injury. Correlation of the molecular biological study results (determining the UCH-L1 and S100B serum concentration by solid phase enzyme immunoassay — ELISA using sets of reagents Sigma-Aldrich, USA, on the 1st day after severe TBI) with the injury type (diffuse or focal according to L. F. Marshall classification) was evaluated.Results. In patients with isolated severe TBI, after exclusion of concomitant extracranial injuries, intoxication and other causes for unconsciousness, UCH-L1 / S100B concentration ratio exceeding the cut-off value of 15.8 indicated a high probability of diffuse injury, ratio less than 15.8 was a marker of focal injury. The sensitivity of the model was 77.8%, specificity — 79.6%.Conclusion. It has been shown that the estimation of serum concentration of neuronal damage biomarker UCH-L1 and astroglial damage biomarker S100B in patients with severe TBI on the 1st day after injury allows diagnose diffuse and focal brain damage with high efficiency. |
| first_indexed | 2024-03-08T12:42:08Z |
| format | Article |
| id | doaj.art-26e4cd7136ee4694892a30974c8adf45 |
| institution | Directory Open Access Journal |
| issn | 2663-9084 2663-9092 |
| language | English |
| last_indexed | 2024-03-08T12:42:08Z |
| publishDate | 2017-12-01 |
| publisher | Romodanov Neurosurgery Institute |
| record_format | Article |
| series | Ukrainian Neurosurgical Journal |
| spelling | doaj.art-26e4cd7136ee4694892a30974c8adf452024-01-21T08:27:08ZengRomodanov Neurosurgery InstituteUkrainian Neurosurgical Journal2663-90842663-90922017-12-014485410.25305/unj.113373The study of UCH-L1 and S100B serum concentration in the diagnosis of diffuse and focal severe traumatic brain injuryOleg Y. Kobyletsky0Lyudmyla M. Bielska1Volodymyr M. Shevaha2Vadym V. Biloshytsky3https://orcid.org/0000-0003-0680-0538Danylo Halytsky Lviv National Medical University, Lviv, UkraineRomodanov Neurosurgery Institute, Kyiv, UkraineDanylo Halytsky Lviv National Medical University, Lviv, UkraineRomodanov Neurosurgery Institute, Kyiv, Ukraine Purpose: To evaluate the feasibility of determining serum concentration of biomarker of neuronal protein damage UCH-L1 and biomarker of astroglia damage S100 on the 1st day after injury, particularly for the diagnosis of focal and diffuse brain impairments based on clinical and biochemical and computer tomographic study in patients with acute severe brain injury.Methods. We used the results of diagnostic tests and therapeutic manipulations in 72 patients aged 16 to 76 years with severe traumatic brain injury. Correlation of the molecular biological study results (determining the UCH-L1 and S100B serum concentration by solid phase enzyme immunoassay — ELISA using sets of reagents Sigma-Aldrich, USA, on the 1st day after severe TBI) with the injury type (diffuse or focal according to L. F. Marshall classification) was evaluated.Results. In patients with isolated severe TBI, after exclusion of concomitant extracranial injuries, intoxication and other causes for unconsciousness, UCH-L1 / S100B concentration ratio exceeding the cut-off value of 15.8 indicated a high probability of diffuse injury, ratio less than 15.8 was a marker of focal injury. The sensitivity of the model was 77.8%, specificity — 79.6%.Conclusion. It has been shown that the estimation of serum concentration of neuronal damage biomarker UCH-L1 and astroglial damage biomarker S100B in patients with severe TBI on the 1st day after injury allows diagnose diffuse and focal brain damage with high efficiency.https://theunj.org/article/view/113373 |
| spellingShingle | Oleg Y. Kobyletsky Lyudmyla M. Bielska Volodymyr M. Shevaha Vadym V. Biloshytsky The study of UCH-L1 and S100B serum concentration in the diagnosis of diffuse and focal severe traumatic brain injury Ukrainian Neurosurgical Journal |
| title | The study of UCH-L1 and S100B serum concentration in the diagnosis of diffuse and focal severe traumatic brain injury |
| title_full | The study of UCH-L1 and S100B serum concentration in the diagnosis of diffuse and focal severe traumatic brain injury |
| title_fullStr | The study of UCH-L1 and S100B serum concentration in the diagnosis of diffuse and focal severe traumatic brain injury |
| title_full_unstemmed | The study of UCH-L1 and S100B serum concentration in the diagnosis of diffuse and focal severe traumatic brain injury |
| title_short | The study of UCH-L1 and S100B serum concentration in the diagnosis of diffuse and focal severe traumatic brain injury |
| title_sort | study of uch l1 and s100b serum concentration in the diagnosis of diffuse and focal severe traumatic brain injury |
| url | https://theunj.org/article/view/113373 |
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