A new set of BXD recombinant inbred lines from advanced intercross populations in mice
<p>Abstract</p> <p>Background</p> <p>Recombinant inbred (RI) strains are an important resource for mapping complex traits in many species. While large RI panels are available for <it>Arabidopsis</it>, maize, <it>C. elegans</it>, and <it>Dro...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2004-04-01
|
| Series: | BMC Genetics |
| Online Access: | http://www.biomedcentral.com/1471-2156/5/7 |
| _version_ | 1818572304695689216 |
|---|---|
| author | Gu Jing Silver Lee M Lu Lu Peirce Jeremy L Williams Robert W |
| author_facet | Gu Jing Silver Lee M Lu Lu Peirce Jeremy L Williams Robert W |
| author_sort | Gu Jing |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Recombinant inbred (RI) strains are an important resource for mapping complex traits in many species. While large RI panels are available for <it>Arabidopsis</it>, maize, <it>C. elegans</it>, and <it>Drosophila</it>, mouse RI panels typically consist of fewer than 30 lines. This is a severe constraint on the power and precision of mapping efforts and greatly hampers analysis of epistatic interactions.</p> <p>Results</p> <p>In order to address these limitations and to provide the community with a more effective collaborative RI mapping panel we generated new BXD RI strains from two independent advanced intercrosses (AI) between C57BL/6J (B6) and DBA/2J (D2) progenitor strains. Progeny were intercrossed for 9 to 14 generations before initiating inbreeding, which is still ongoing for some strains. Since this AI base population is highly recombinant, the 46 advanced recombinant inbred (ARI) strains incorporate approximately twice as many recombinations as standard RI strains, a fraction of which are inevitably shared by descent. When combined with the existing BXD RI strains, the merged BXD strain set triples the number of previously available unique recombinations and quadruples the total number of recombinations in the BXD background.</p> <p>Conclusion</p> <p>The combined BXD strain set is the largest mouse RI mapping panel. It is a powerful tool for collaborative analysis of quantitative traits and gene function that will be especially useful to study variation in transcriptome and proteome data sets under multiple environments. Additional strains also extend the value of the extensive phenotypic characterization of the previously available strains. A final advantage of expanding the BXD strain set is that both progenitors have been sequenced, and approximately 1.8 million SNPs have been characterized. This provides unprecedented power in screening candidate genes and can reduce the effective length of QTL intervals. It also makes it possible to reverse standard mapping strategies and to explore downstream effects of known sequence variants.</p> |
| first_indexed | 2024-12-14T18:55:22Z |
| format | Article |
| id | doaj.art-26e5f8ca6dbf4b60b59b39c9153a3cae |
| institution | Directory Open Access Journal |
| issn | 1471-2156 |
| language | English |
| last_indexed | 2024-12-14T18:55:22Z |
| publishDate | 2004-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genetics |
| spelling | doaj.art-26e5f8ca6dbf4b60b59b39c9153a3cae2022-12-21T22:51:07ZengBMCBMC Genetics1471-21562004-04-0151710.1186/1471-2156-5-7A new set of BXD recombinant inbred lines from advanced intercross populations in miceGu JingSilver Lee MLu LuPeirce Jeremy LWilliams Robert W<p>Abstract</p> <p>Background</p> <p>Recombinant inbred (RI) strains are an important resource for mapping complex traits in many species. While large RI panels are available for <it>Arabidopsis</it>, maize, <it>C. elegans</it>, and <it>Drosophila</it>, mouse RI panels typically consist of fewer than 30 lines. This is a severe constraint on the power and precision of mapping efforts and greatly hampers analysis of epistatic interactions.</p> <p>Results</p> <p>In order to address these limitations and to provide the community with a more effective collaborative RI mapping panel we generated new BXD RI strains from two independent advanced intercrosses (AI) between C57BL/6J (B6) and DBA/2J (D2) progenitor strains. Progeny were intercrossed for 9 to 14 generations before initiating inbreeding, which is still ongoing for some strains. Since this AI base population is highly recombinant, the 46 advanced recombinant inbred (ARI) strains incorporate approximately twice as many recombinations as standard RI strains, a fraction of which are inevitably shared by descent. When combined with the existing BXD RI strains, the merged BXD strain set triples the number of previously available unique recombinations and quadruples the total number of recombinations in the BXD background.</p> <p>Conclusion</p> <p>The combined BXD strain set is the largest mouse RI mapping panel. It is a powerful tool for collaborative analysis of quantitative traits and gene function that will be especially useful to study variation in transcriptome and proteome data sets under multiple environments. Additional strains also extend the value of the extensive phenotypic characterization of the previously available strains. A final advantage of expanding the BXD strain set is that both progenitors have been sequenced, and approximately 1.8 million SNPs have been characterized. This provides unprecedented power in screening candidate genes and can reduce the effective length of QTL intervals. It also makes it possible to reverse standard mapping strategies and to explore downstream effects of known sequence variants.</p>http://www.biomedcentral.com/1471-2156/5/7 |
| spellingShingle | Gu Jing Silver Lee M Lu Lu Peirce Jeremy L Williams Robert W A new set of BXD recombinant inbred lines from advanced intercross populations in mice BMC Genetics |
| title | A new set of BXD recombinant inbred lines from advanced intercross populations in mice |
| title_full | A new set of BXD recombinant inbred lines from advanced intercross populations in mice |
| title_fullStr | A new set of BXD recombinant inbred lines from advanced intercross populations in mice |
| title_full_unstemmed | A new set of BXD recombinant inbred lines from advanced intercross populations in mice |
| title_short | A new set of BXD recombinant inbred lines from advanced intercross populations in mice |
| title_sort | new set of bxd recombinant inbred lines from advanced intercross populations in mice |
| url | http://www.biomedcentral.com/1471-2156/5/7 |
| work_keys_str_mv | AT gujing anewsetofbxdrecombinantinbredlinesfromadvancedintercrosspopulationsinmice AT silverleem anewsetofbxdrecombinantinbredlinesfromadvancedintercrosspopulationsinmice AT lulu anewsetofbxdrecombinantinbredlinesfromadvancedintercrosspopulationsinmice AT peircejeremyl anewsetofbxdrecombinantinbredlinesfromadvancedintercrosspopulationsinmice AT williamsrobertw anewsetofbxdrecombinantinbredlinesfromadvancedintercrosspopulationsinmice AT gujing newsetofbxdrecombinantinbredlinesfromadvancedintercrosspopulationsinmice AT silverleem newsetofbxdrecombinantinbredlinesfromadvancedintercrosspopulationsinmice AT lulu newsetofbxdrecombinantinbredlinesfromadvancedintercrosspopulationsinmice AT peircejeremyl newsetofbxdrecombinantinbredlinesfromadvancedintercrosspopulationsinmice AT williamsrobertw newsetofbxdrecombinantinbredlinesfromadvancedintercrosspopulationsinmice |