Hydrothermal assisted biogenic synthesis of silver nanoparticles: A potential study on virulent candida isolates from COVID-19 patients
Till now the exact mechanism and effect of biogenic silver nanoparticles on fungus is an indefinable question. To focus on this issue, the first time we prepared hydrothermal assisted thyme coated silver nanoparticles (T/AgNPs) and their toxic effect on Candida isolates were determined. The role of...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2022-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9536612/?tool=EBI |
| _version_ | 1817986453888565248 |
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| author | Fatma O. Khalil Muhammad B. Taj Enas M. Ghonaim Shimaa Abed El-Sattar Sally W. Elkhadry Hala El-Refai Omar M. Ali Ahmed Salah A. Elgawad Heba Alshater |
| author_facet | Fatma O. Khalil Muhammad B. Taj Enas M. Ghonaim Shimaa Abed El-Sattar Sally W. Elkhadry Hala El-Refai Omar M. Ali Ahmed Salah A. Elgawad Heba Alshater |
| author_sort | Fatma O. Khalil |
| collection | DOAJ |
| description | Till now the exact mechanism and effect of biogenic silver nanoparticles on fungus is an indefinable question. To focus on this issue, the first time we prepared hydrothermal assisted thyme coated silver nanoparticles (T/AgNPs) and their toxic effect on Candida isolates were determined. The role of thyme (Thymus Vulgaris) in the reduction of silver ions and stabilization of T/AgNPs was estimated by Fourier transforms infrared spectroscopy, structure and size of present silver nanoparticles were detected via atomic force microscopy as well as high-resolution transmission electron microscopy. The biological activity of T/AgNPs was observed against Candida isolates from COVID-19 Patients. Testing of virulence of Candida species using Multiplex PCR. T/AgNPs proved highly effective against Candida albicans, Candida kruzei, Candida glabrata and MIC values ranging from 156.25 to 1,250 μg/mL and MFC values ranging from 312.5 to 5,000 μg/mL. The structural and morphological modifications due to T/AgNPs on Candida albicans were detected by TEM. It was highly observed that when Candida albicans cells were subjected to 50 and 100 μg/mL T/AgNPs, a remarkable change in the cell wall and cell membrane was observed. |
| first_indexed | 2024-04-14T00:09:21Z |
| format | Article |
| id | doaj.art-26e84307476c4777b397bc6900d9d8e0 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-14T00:09:21Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-26e84307476c4777b397bc6900d9d8e02022-12-22T02:23:24ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-011710Hydrothermal assisted biogenic synthesis of silver nanoparticles: A potential study on virulent candida isolates from COVID-19 patientsFatma O. KhalilMuhammad B. TajEnas M. GhonaimShimaa Abed El-SattarSally W. ElkhadryHala El-RefaiOmar M. AliAhmed Salah A. ElgawadHeba AlshaterTill now the exact mechanism and effect of biogenic silver nanoparticles on fungus is an indefinable question. To focus on this issue, the first time we prepared hydrothermal assisted thyme coated silver nanoparticles (T/AgNPs) and their toxic effect on Candida isolates were determined. The role of thyme (Thymus Vulgaris) in the reduction of silver ions and stabilization of T/AgNPs was estimated by Fourier transforms infrared spectroscopy, structure and size of present silver nanoparticles were detected via atomic force microscopy as well as high-resolution transmission electron microscopy. The biological activity of T/AgNPs was observed against Candida isolates from COVID-19 Patients. Testing of virulence of Candida species using Multiplex PCR. T/AgNPs proved highly effective against Candida albicans, Candida kruzei, Candida glabrata and MIC values ranging from 156.25 to 1,250 μg/mL and MFC values ranging from 312.5 to 5,000 μg/mL. The structural and morphological modifications due to T/AgNPs on Candida albicans were detected by TEM. It was highly observed that when Candida albicans cells were subjected to 50 and 100 μg/mL T/AgNPs, a remarkable change in the cell wall and cell membrane was observed.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9536612/?tool=EBI |
| spellingShingle | Fatma O. Khalil Muhammad B. Taj Enas M. Ghonaim Shimaa Abed El-Sattar Sally W. Elkhadry Hala El-Refai Omar M. Ali Ahmed Salah A. Elgawad Heba Alshater Hydrothermal assisted biogenic synthesis of silver nanoparticles: A potential study on virulent candida isolates from COVID-19 patients PLoS ONE |
| title | Hydrothermal assisted biogenic synthesis of silver nanoparticles: A potential study on virulent candida isolates from COVID-19 patients |
| title_full | Hydrothermal assisted biogenic synthesis of silver nanoparticles: A potential study on virulent candida isolates from COVID-19 patients |
| title_fullStr | Hydrothermal assisted biogenic synthesis of silver nanoparticles: A potential study on virulent candida isolates from COVID-19 patients |
| title_full_unstemmed | Hydrothermal assisted biogenic synthesis of silver nanoparticles: A potential study on virulent candida isolates from COVID-19 patients |
| title_short | Hydrothermal assisted biogenic synthesis of silver nanoparticles: A potential study on virulent candida isolates from COVID-19 patients |
| title_sort | hydrothermal assisted biogenic synthesis of silver nanoparticles a potential study on virulent candida isolates from covid 19 patients |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9536612/?tool=EBI |
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