Development of a semi-open chamber system for the gas exchange measurement of whole-canopy under steady and unsteady states in cucumber seedlings
Abstract Background Large-scale data on the photosynthetic characteristics of whole crop canopy is crucial for improving yield. However, current data collection methods remain challenging, and the time constraints associated with photosynthetic data collection further complicate matters. Developing...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-08-01
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| Series: | Plant Methods |
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| Online Access: | https://doi.org/10.1186/s13007-023-01059-1 |
| _version_ | 1797559455513051136 |
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| author | Yu Hyun Moon Ui Jeong Woo Ha Seon Sim Tae Yeon Lee Ha Rang Shin Jung Su Jo Sung Kyeom Kim |
| author_facet | Yu Hyun Moon Ui Jeong Woo Ha Seon Sim Tae Yeon Lee Ha Rang Shin Jung Su Jo Sung Kyeom Kim |
| author_sort | Yu Hyun Moon |
| collection | DOAJ |
| description | Abstract Background Large-scale data on the photosynthetic characteristics of whole crop canopy is crucial for improving yield. However, current data collection methods remain challenging, and the time constraints associated with photosynthetic data collection further complicate matters. Developing a practical yet easy-to-use tool for collecting whole-canopy data is essential to address these challenges. Furthermore, it is necessary to obtain instantaneous measurements of photosynthetic rate over a wide range of CO2 concentrations under an unsteady state to enable faster data collection and obtain reliable biochemical limits of carbon assimilation. This study developed a semi-open chamber system with steady and unsteady state measurement techniques to collect biochemical photosynthetic data from an entire cucumber canopy, emphasizing the correction procedures for CO2 concentration of unsteady state measurements applicable regardless of chamber scale. Results After constructing a semi-open chamber system, we described how to correct measurement errors according to chamber volume. In order to assess the accuracy of the newly developed system, an analysis was conducted to determine the overall measurement error resulting from variations in the reference, sample CO2 concentration, and leakage flow rate. The total measurement error was accurate to no more than 10%. Furthermore, the difference between the photosynthetic rate of the single leaf and that of the whole-canopy was not significant in Rubisco activity-limited carboxylation range. In addition, the Farquhar–von Caemmerer–Berry (FvCB) model parameters and the photosynthetic rate estimation values were compared to evaluate the steady- and unsteady state measurement methods between the cucumber seedlings' single-leaf and whole-canopy. The average root mean square error of the FvCB model in the steady (standard A-Ci response) and unsteady states (800 to 400 ramp) of the chambers was 1.4 and 2.3, respectively. Results show that the developed system is suitable for measuring the gas exchange rate of the cucumber canopy. Conclusions We demonstrate the correction method for measurement errors to enable the gas exchange rate of the whole-canopy even in an unsteady state. The correction method of the measurement system of the gas exchange rate for the whole- canopy can be applied regardless of the volume of the chamber, and it can be applied simply to other chamber systems. In addition, an unsteady state measurement method for fast data collection was also applicable. However, it was deemed necessary to identify a more optimal measurement range by conducting measurements across a broader range of values. |
| first_indexed | 2024-03-10T17:45:41Z |
| format | Article |
| id | doaj.art-26e91041a3dc459288ce68d4bbd81fe1 |
| institution | Directory Open Access Journal |
| issn | 1746-4811 |
| language | English |
| last_indexed | 2024-03-10T17:45:41Z |
| publishDate | 2023-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Plant Methods |
| spelling | doaj.art-26e91041a3dc459288ce68d4bbd81fe12023-11-20T09:32:37ZengBMCPlant Methods1746-48112023-08-0119111410.1186/s13007-023-01059-1Development of a semi-open chamber system for the gas exchange measurement of whole-canopy under steady and unsteady states in cucumber seedlingsYu Hyun Moon0Ui Jeong Woo1Ha Seon Sim2Tae Yeon Lee3Ha Rang Shin4Jung Su Jo5Sung Kyeom Kim6Department of Horticultural Science, College of Agricultural and Science, Kyungpook National UniversityDepartment of Horticultural Science, College of Agricultural and Science, Kyungpook National UniversityDepartment of Horticultural Science, College of Agricultural and Science, Kyungpook National UniversityDepartment of Horticultural Science, College of Agricultural and Science, Kyungpook National UniversityDepartment of Horticultural Science, College of Agricultural and Science, Kyungpook National UniversityInstitute of Agricultural Science and Technology, Kyungpook National UniversityDepartment of Horticultural Science, College of Agricultural and Science, Kyungpook National UniversityAbstract Background Large-scale data on the photosynthetic characteristics of whole crop canopy is crucial for improving yield. However, current data collection methods remain challenging, and the time constraints associated with photosynthetic data collection further complicate matters. Developing a practical yet easy-to-use tool for collecting whole-canopy data is essential to address these challenges. Furthermore, it is necessary to obtain instantaneous measurements of photosynthetic rate over a wide range of CO2 concentrations under an unsteady state to enable faster data collection and obtain reliable biochemical limits of carbon assimilation. This study developed a semi-open chamber system with steady and unsteady state measurement techniques to collect biochemical photosynthetic data from an entire cucumber canopy, emphasizing the correction procedures for CO2 concentration of unsteady state measurements applicable regardless of chamber scale. Results After constructing a semi-open chamber system, we described how to correct measurement errors according to chamber volume. In order to assess the accuracy of the newly developed system, an analysis was conducted to determine the overall measurement error resulting from variations in the reference, sample CO2 concentration, and leakage flow rate. The total measurement error was accurate to no more than 10%. Furthermore, the difference between the photosynthetic rate of the single leaf and that of the whole-canopy was not significant in Rubisco activity-limited carboxylation range. In addition, the Farquhar–von Caemmerer–Berry (FvCB) model parameters and the photosynthetic rate estimation values were compared to evaluate the steady- and unsteady state measurement methods between the cucumber seedlings' single-leaf and whole-canopy. The average root mean square error of the FvCB model in the steady (standard A-Ci response) and unsteady states (800 to 400 ramp) of the chambers was 1.4 and 2.3, respectively. Results show that the developed system is suitable for measuring the gas exchange rate of the cucumber canopy. Conclusions We demonstrate the correction method for measurement errors to enable the gas exchange rate of the whole-canopy even in an unsteady state. The correction method of the measurement system of the gas exchange rate for the whole- canopy can be applied regardless of the volume of the chamber, and it can be applied simply to other chamber systems. In addition, an unsteady state measurement method for fast data collection was also applicable. However, it was deemed necessary to identify a more optimal measurement range by conducting measurements across a broader range of values.https://doi.org/10.1186/s13007-023-01059-1CO2 assimilation rateCucumber seedlingsGas exchange ratePhotosynthesis modelSemi-open chamberWhole-canopy |
| spellingShingle | Yu Hyun Moon Ui Jeong Woo Ha Seon Sim Tae Yeon Lee Ha Rang Shin Jung Su Jo Sung Kyeom Kim Development of a semi-open chamber system for the gas exchange measurement of whole-canopy under steady and unsteady states in cucumber seedlings Plant Methods CO2 assimilation rate Cucumber seedlings Gas exchange rate Photosynthesis model Semi-open chamber Whole-canopy |
| title | Development of a semi-open chamber system for the gas exchange measurement of whole-canopy under steady and unsteady states in cucumber seedlings |
| title_full | Development of a semi-open chamber system for the gas exchange measurement of whole-canopy under steady and unsteady states in cucumber seedlings |
| title_fullStr | Development of a semi-open chamber system for the gas exchange measurement of whole-canopy under steady and unsteady states in cucumber seedlings |
| title_full_unstemmed | Development of a semi-open chamber system for the gas exchange measurement of whole-canopy under steady and unsteady states in cucumber seedlings |
| title_short | Development of a semi-open chamber system for the gas exchange measurement of whole-canopy under steady and unsteady states in cucumber seedlings |
| title_sort | development of a semi open chamber system for the gas exchange measurement of whole canopy under steady and unsteady states in cucumber seedlings |
| topic | CO2 assimilation rate Cucumber seedlings Gas exchange rate Photosynthesis model Semi-open chamber Whole-canopy |
| url | https://doi.org/10.1186/s13007-023-01059-1 |
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