Anticandidal and Antibiofilm Effect of Synbiotics including Probiotics and Inulin-Type Fructans
Background: There is great interest in the search for new alternatives to antimicrobial drugs, and the use of synbiotics is a promising approach to this problem. This study evaluated the growth inhibition and antibiofilm activity of the short-chain fatty acids produced by <i>Lacticaseibacillus...
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| Format: | Article |
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MDPI AG
2022-08-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/11/8/1135 |
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| author | Ricardo García-Gamboa Miguel Domínguez-Simi Misael S. Gradilla-Hernández Jorge Bravo Andrés Moya Blanca Ruiz-Álvarez Marisela González-Avila |
| author_facet | Ricardo García-Gamboa Miguel Domínguez-Simi Misael S. Gradilla-Hernández Jorge Bravo Andrés Moya Blanca Ruiz-Álvarez Marisela González-Avila |
| author_sort | Ricardo García-Gamboa |
| collection | DOAJ |
| description | Background: There is great interest in the search for new alternatives to antimicrobial drugs, and the use of synbiotics is a promising approach to this problem. This study evaluated the growth inhibition and antibiofilm activity of the short-chain fatty acids produced by <i>Lacticaseibacillus rhamnosus</i> and <i>Pediococcus acidilactici</i> in combination with inulin-type fructans against <i>Candida albicans</i>. Methods: The growth inhibition of <i>Candida</i> was evaluated using microdilution analysis in 96-well microtiter plates; different concentrations of cell-free supernatants of <i>Lacticaseibacillus rhamnosus</i> and <i>Pediococcus acidilactici</i> were exposed to <i>Candida albicans</i>. The antibiofilm assessment was carried out using the crystal violet staining assay. The short-chain fatty acids were analyzed by gas chromatography. Results: The clinically isolated <i>Candida albicans</i> interacted with supernatants from <i>Lacticaseibacillus rhamnosus</i> and <i>Pediococcus acidilactici</i> and showed significant growth inhibition and antibiofilm formation versus the controls. Lactate and acetic acid were elevated in the supernatants. The results suggest that the supernatants obtained from the synbiotic combinations of <i>Lacticaseibacillus rhamnosus</i> and <i>Pediococcus acidilactici</i> with inulin-type fructans can inhibit the growth and biofilm formation against a clinically isolated <i>Candida albicans</i> strain. Conclusions: These results suggest that synbiotic formulations could be a promising alternative to antifungal drugs in candidiasis therapy. |
| first_indexed | 2024-03-09T04:46:37Z |
| format | Article |
| id | doaj.art-26ee469ec07c49ddbd061de3a772d859 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-09T04:46:37Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-26ee469ec07c49ddbd061de3a772d8592023-12-03T13:15:06ZengMDPI AGAntibiotics2079-63822022-08-01118113510.3390/antibiotics11081135Anticandidal and Antibiofilm Effect of Synbiotics including Probiotics and Inulin-Type FructansRicardo García-Gamboa0Miguel Domínguez-Simi1Misael S. Gradilla-Hernández2Jorge Bravo3Andrés Moya4Blanca Ruiz-Álvarez5Marisela González-Avila6Centre for Research and Assistance in Technology and Design of the State of Jalisco A.C., Normalistas 800, Guadalajara 44270, MexicoCentre for Research and Assistance in Technology and Design of the State of Jalisco A.C., Normalistas 800, Guadalajara 44270, MexicoTecnológico de Monterrey, Escuela de Ingeniería y Ciencias, Av. General Ramón Corona 2514, Nuevo Mexico, Zapopan 45138, MexicoCentre for Research and Assistance in Technology and Design of the State of Jalisco A.C., Normalistas 800, Guadalajara 44270, MexicoFoundation for the Promotion of Health and Biomedical Research in the Valencian Community Public Health (FISABIO), 46020 Valencia, SpainCentre for Research and Assistance in Technology and Design of the State of Jalisco A.C., Normalistas 800, Guadalajara 44270, MexicoCentre for Research and Assistance in Technology and Design of the State of Jalisco A.C., Normalistas 800, Guadalajara 44270, MexicoBackground: There is great interest in the search for new alternatives to antimicrobial drugs, and the use of synbiotics is a promising approach to this problem. This study evaluated the growth inhibition and antibiofilm activity of the short-chain fatty acids produced by <i>Lacticaseibacillus rhamnosus</i> and <i>Pediococcus acidilactici</i> in combination with inulin-type fructans against <i>Candida albicans</i>. Methods: The growth inhibition of <i>Candida</i> was evaluated using microdilution analysis in 96-well microtiter plates; different concentrations of cell-free supernatants of <i>Lacticaseibacillus rhamnosus</i> and <i>Pediococcus acidilactici</i> were exposed to <i>Candida albicans</i>. The antibiofilm assessment was carried out using the crystal violet staining assay. The short-chain fatty acids were analyzed by gas chromatography. Results: The clinically isolated <i>Candida albicans</i> interacted with supernatants from <i>Lacticaseibacillus rhamnosus</i> and <i>Pediococcus acidilactici</i> and showed significant growth inhibition and antibiofilm formation versus the controls. Lactate and acetic acid were elevated in the supernatants. The results suggest that the supernatants obtained from the synbiotic combinations of <i>Lacticaseibacillus rhamnosus</i> and <i>Pediococcus acidilactici</i> with inulin-type fructans can inhibit the growth and biofilm formation against a clinically isolated <i>Candida albicans</i> strain. Conclusions: These results suggest that synbiotic formulations could be a promising alternative to antifungal drugs in candidiasis therapy.https://www.mdpi.com/2079-6382/11/8/1135biofilms<i>Candida</i>probioticsinulin-type fructansantimicrobial activityantibiofilm effect |
| spellingShingle | Ricardo García-Gamboa Miguel Domínguez-Simi Misael S. Gradilla-Hernández Jorge Bravo Andrés Moya Blanca Ruiz-Álvarez Marisela González-Avila Anticandidal and Antibiofilm Effect of Synbiotics including Probiotics and Inulin-Type Fructans Antibiotics biofilms <i>Candida</i> probiotics inulin-type fructans antimicrobial activity antibiofilm effect |
| title | Anticandidal and Antibiofilm Effect of Synbiotics including Probiotics and Inulin-Type Fructans |
| title_full | Anticandidal and Antibiofilm Effect of Synbiotics including Probiotics and Inulin-Type Fructans |
| title_fullStr | Anticandidal and Antibiofilm Effect of Synbiotics including Probiotics and Inulin-Type Fructans |
| title_full_unstemmed | Anticandidal and Antibiofilm Effect of Synbiotics including Probiotics and Inulin-Type Fructans |
| title_short | Anticandidal and Antibiofilm Effect of Synbiotics including Probiotics and Inulin-Type Fructans |
| title_sort | anticandidal and antibiofilm effect of synbiotics including probiotics and inulin type fructans |
| topic | biofilms <i>Candida</i> probiotics inulin-type fructans antimicrobial activity antibiofilm effect |
| url | https://www.mdpi.com/2079-6382/11/8/1135 |
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