Phenotypic, molecular detection, and Antibiotic Resistance Profile (MDR and XDR) of Aeromonas hydrophila isolated from Farmed Tilapia zillii and Mugil cephalus
Abstract In the present study, Aeromonas hydrophila was isolated from Tilapia zillii and Mugil cephalus samples collected during different seasons from various Suez Canal areas in Egypt. The prevalence of A. hydrophila, virulence genes, and antibiotic resistance profile of the isolates to the common...
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| Format: | Article |
| Language: | English |
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BMC
2024-03-01
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| Series: | BMC Veterinary Research |
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| Online Access: | https://doi.org/10.1186/s12917-024-03942-y |
| _version_ | 1797266751600197632 |
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| author | Hala F. Ayoub Ahmed R. khafagy Aboelkair M. Esawy Noura Abo El-moaty Khairiah Mubarak Alwutayd Abdallah Tageldein Mansour Reham A. Ibrahim Dalia A. Abdel-moneam Reham M. El-Tarabili |
| author_facet | Hala F. Ayoub Ahmed R. khafagy Aboelkair M. Esawy Noura Abo El-moaty Khairiah Mubarak Alwutayd Abdallah Tageldein Mansour Reham A. Ibrahim Dalia A. Abdel-moneam Reham M. El-Tarabili |
| author_sort | Hala F. Ayoub |
| collection | DOAJ |
| description | Abstract In the present study, Aeromonas hydrophila was isolated from Tilapia zillii and Mugil cephalus samples collected during different seasons from various Suez Canal areas in Egypt. The prevalence of A. hydrophila, virulence genes, and antibiotic resistance profile of the isolates to the commonly used antibiotics in aquaculture were investigated to identify multiple drug resistance (MDR) and extensive drug-resistant (XDR) strains. In addition, a pathogenicity test was conducted using A. hydrophila, which was isolated and selected based on the prevalence of virulence and resistance genes, and morbidity of natural infected fish. The results revealed that A. hydrophila was isolated from 38 of the 120 collected fish samples (31.6%) and confirmed phenotypically and biochemically. Several virulence genes were detected in retrieved A. hydrophila isolates, including aerolysin aerA (57.9%), ser (28.9%), alt (26.3%), ast (13.1%), act (7.9%), hlyA (7.9%), and nuc (18.4%). Detection of antibiotic-resistant genes revealed that all isolates were positive for bla pse1 (100%), bla SHV (42.1%), tetA (60.5%), and sul1 (42.1%). 63.1% of recovered isolates were considered MDR, while 28.9% of recovered isolates were considered XDR. Some isolates harbor both virulence and MDR genes; the highest percentage carried 11, followed by isolates harboring 9 virulence and resistance genes. It could be concluded that the high prevalence of A. hydrophila in aquaculture species and their diverse antibiotic resistance and virulence genes suggest the high risk of Aeromonas infection and could have important implications for aquaculture and public health. |
| first_indexed | 2024-04-25T01:05:40Z |
| format | Article |
| id | doaj.art-26efe0d09a8548888ac6360564f1ac24 |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-04-25T01:05:40Z |
| publishDate | 2024-03-01 |
| publisher | BMC |
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| series | BMC Veterinary Research |
| spelling | doaj.art-26efe0d09a8548888ac6360564f1ac242024-03-10T12:15:04ZengBMCBMC Veterinary Research1746-61482024-03-0120111310.1186/s12917-024-03942-yPhenotypic, molecular detection, and Antibiotic Resistance Profile (MDR and XDR) of Aeromonas hydrophila isolated from Farmed Tilapia zillii and Mugil cephalusHala F. Ayoub0Ahmed R. khafagy1Aboelkair M. Esawy2Noura Abo El-moaty3Khairiah Mubarak Alwutayd4Abdallah Tageldein Mansour5Reham A. Ibrahim6Dalia A. Abdel-moneam7Reham M. El-Tarabili8Department of Fish Health and Management, Central Laboratory for Aquaculture Research (CLAR), Agricultural Research CenterDepartment of Bacteriology, Immunology, and Mycology, Faculty of Veterinary Medicine, Ain Shams UniversityDepartment of Microbiology, Animal Health Research InstituteDepartment of Microbiology, Animal Health Research InstituteDepartment of Biology, College of Science, Princess Nourah bint Abdulrahman UniversityFish and Animal Production and Aquaculture Department, College of Agriculture and Food Sciences, King Faisal UniversityMicrobiology Department, National Institute of Oceanography and Fisheries (NIOF)Department of Aquatic Animal Medicine and Management, Faculty of Veterinary Medicine, Cairo UniversityDepartment of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Suez Canal UniversityAbstract In the present study, Aeromonas hydrophila was isolated from Tilapia zillii and Mugil cephalus samples collected during different seasons from various Suez Canal areas in Egypt. The prevalence of A. hydrophila, virulence genes, and antibiotic resistance profile of the isolates to the commonly used antibiotics in aquaculture were investigated to identify multiple drug resistance (MDR) and extensive drug-resistant (XDR) strains. In addition, a pathogenicity test was conducted using A. hydrophila, which was isolated and selected based on the prevalence of virulence and resistance genes, and morbidity of natural infected fish. The results revealed that A. hydrophila was isolated from 38 of the 120 collected fish samples (31.6%) and confirmed phenotypically and biochemically. Several virulence genes were detected in retrieved A. hydrophila isolates, including aerolysin aerA (57.9%), ser (28.9%), alt (26.3%), ast (13.1%), act (7.9%), hlyA (7.9%), and nuc (18.4%). Detection of antibiotic-resistant genes revealed that all isolates were positive for bla pse1 (100%), bla SHV (42.1%), tetA (60.5%), and sul1 (42.1%). 63.1% of recovered isolates were considered MDR, while 28.9% of recovered isolates were considered XDR. Some isolates harbor both virulence and MDR genes; the highest percentage carried 11, followed by isolates harboring 9 virulence and resistance genes. It could be concluded that the high prevalence of A. hydrophila in aquaculture species and their diverse antibiotic resistance and virulence genes suggest the high risk of Aeromonas infection and could have important implications for aquaculture and public health.https://doi.org/10.1186/s12917-024-03942-yPrevalenceAeromonas hydrophilaVirulence genesAntibiotic resistance, cultured freshwater fish |
| spellingShingle | Hala F. Ayoub Ahmed R. khafagy Aboelkair M. Esawy Noura Abo El-moaty Khairiah Mubarak Alwutayd Abdallah Tageldein Mansour Reham A. Ibrahim Dalia A. Abdel-moneam Reham M. El-Tarabili Phenotypic, molecular detection, and Antibiotic Resistance Profile (MDR and XDR) of Aeromonas hydrophila isolated from Farmed Tilapia zillii and Mugil cephalus BMC Veterinary Research Prevalence Aeromonas hydrophila Virulence genes Antibiotic resistance, cultured freshwater fish |
| title | Phenotypic, molecular detection, and Antibiotic Resistance Profile (MDR and XDR) of Aeromonas hydrophila isolated from Farmed Tilapia zillii and Mugil cephalus |
| title_full | Phenotypic, molecular detection, and Antibiotic Resistance Profile (MDR and XDR) of Aeromonas hydrophila isolated from Farmed Tilapia zillii and Mugil cephalus |
| title_fullStr | Phenotypic, molecular detection, and Antibiotic Resistance Profile (MDR and XDR) of Aeromonas hydrophila isolated from Farmed Tilapia zillii and Mugil cephalus |
| title_full_unstemmed | Phenotypic, molecular detection, and Antibiotic Resistance Profile (MDR and XDR) of Aeromonas hydrophila isolated from Farmed Tilapia zillii and Mugil cephalus |
| title_short | Phenotypic, molecular detection, and Antibiotic Resistance Profile (MDR and XDR) of Aeromonas hydrophila isolated from Farmed Tilapia zillii and Mugil cephalus |
| title_sort | phenotypic molecular detection and antibiotic resistance profile mdr and xdr of aeromonas hydrophila isolated from farmed tilapia zillii and mugil cephalus |
| topic | Prevalence Aeromonas hydrophila Virulence genes Antibiotic resistance, cultured freshwater fish |
| url | https://doi.org/10.1186/s12917-024-03942-y |
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