Topical hydrogel containing Fumaria vaillantii Loisel. extract enhances wound healing in rats
Abstract Background Fumaria species (Fumariacea) has traditionally been used in wound healing in Iranian folk medicine. However, with the discovery of newer agents, its use has faded off into total obscurity. This study explored the wound healing potential of a gel containing 10% Fumaria vaillantii...
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| Format: | Article |
| Language: | English |
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BMC
2019-09-01
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| Series: | BMC Complementary and Alternative Medicine |
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| Online Access: | http://link.springer.com/article/10.1186/s12906-019-2645-y |
| _version_ | 1818970396166193152 |
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| author | Fatemeh Davoodi-Roodbordeii Minoo Afshar Fatemeh Haji Abas Tabrizi Samira Choopani Giti Torkaman Fariborz Moayer Mona Salimi |
| author_facet | Fatemeh Davoodi-Roodbordeii Minoo Afshar Fatemeh Haji Abas Tabrizi Samira Choopani Giti Torkaman Fariborz Moayer Mona Salimi |
| author_sort | Fatemeh Davoodi-Roodbordeii |
| collection | DOAJ |
| description | Abstract Background Fumaria species (Fumariacea) has traditionally been used in wound healing in Iranian folk medicine. However, with the discovery of newer agents, its use has faded off into total obscurity. This study explored the wound healing potential of a gel containing 10% Fumaria vaillantii Loisel through topical application of total extract in a model of excisional as well as incisional wound healing in albino Wistar rats. Methods Rats were anesthetized, and excisional skin wound was established using a sterilized surgical scissors. The animals were then treated with 10% F.vaillantii topical gel formulation along with the gel base. The treatments were administered once a day after the injury for 21 days. For topical treatment, the hydrogel was formulated and evaluated for chemical and physical characteristics. Histopathological analysis with hematoxylin and eosin (H&E) was used for microscopic examination of the skin tissues on 21-day-old sections of excision wound. To verify collagen formation, hydroxyproline determination was performed 21 days post wound healing. Breaking strength was determined in a 10-day-old incision wound by the uniaxial tensile test. Results Topical administration of F.vaillantii gel formulation significantly enhanced skin wound closure on the 6th post-wounding day compared to both gel base and the negative control, indicating an accelerated wound healing process, while a significant difference was observed on 10th and 14th post –wound days in F.vaillantii treatment compared to the negative control groups. Gel formulation prepared with a 10% F. vaillantii extract exhibited a response in terms of wound epithelialization, angiogenesis and number of hair follicles at wound area better than the gel base on the 21st post-wound day. Application of gel base produced further advantages by increasing hydroxyproline content and collagen fiber thickness. Our results on incision wound model were supported by histopathological data indicating the role of gel base in the enhancement of breaking strength. Conclusion Traditional use of Fumaria species in the skin diseases was justified in this study by revealing the increase in wound healing activity after hydrogel containing F. vaillantii total extract administration. Graphical abstract |
| first_indexed | 2024-12-20T14:35:49Z |
| format | Article |
| id | doaj.art-26fb3de7558a48d6af4fd8147a8d0c82 |
| institution | Directory Open Access Journal |
| issn | 1472-6882 |
| language | English |
| last_indexed | 2024-12-20T14:35:49Z |
| publishDate | 2019-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary and Alternative Medicine |
| spelling | doaj.art-26fb3de7558a48d6af4fd8147a8d0c822022-12-21T19:37:28ZengBMCBMC Complementary and Alternative Medicine1472-68822019-09-011911910.1186/s12906-019-2645-yTopical hydrogel containing Fumaria vaillantii Loisel. extract enhances wound healing in ratsFatemeh Davoodi-Roodbordeii0Minoo Afshar1Fatemeh Haji Abas Tabrizi2Samira Choopani3Giti Torkaman4Fariborz Moayer5Mona Salimi6Department of pharmaceutics, Pharmaceutical Sciences Branch, Islamic Azad University (IAUPS)Department of pharmaceutics, Pharmaceutical Sciences Branch, Islamic Azad University (IAUPS)Department of Physiology and Pharmacology, Pasteur Institute of IranDepartment of Physiology and Pharmacology, Pasteur Institute of IranPhysical Therapy Department, Faculty of Medical Sciences, Tarbiat Modares UniversityDepartment of Pathobiology, College of Veterinary Medicine, Karaj Branch, Islamic Azad UniversityDepartment of Physiology and Pharmacology, Pasteur Institute of IranAbstract Background Fumaria species (Fumariacea) has traditionally been used in wound healing in Iranian folk medicine. However, with the discovery of newer agents, its use has faded off into total obscurity. This study explored the wound healing potential of a gel containing 10% Fumaria vaillantii Loisel through topical application of total extract in a model of excisional as well as incisional wound healing in albino Wistar rats. Methods Rats were anesthetized, and excisional skin wound was established using a sterilized surgical scissors. The animals were then treated with 10% F.vaillantii topical gel formulation along with the gel base. The treatments were administered once a day after the injury for 21 days. For topical treatment, the hydrogel was formulated and evaluated for chemical and physical characteristics. Histopathological analysis with hematoxylin and eosin (H&E) was used for microscopic examination of the skin tissues on 21-day-old sections of excision wound. To verify collagen formation, hydroxyproline determination was performed 21 days post wound healing. Breaking strength was determined in a 10-day-old incision wound by the uniaxial tensile test. Results Topical administration of F.vaillantii gel formulation significantly enhanced skin wound closure on the 6th post-wounding day compared to both gel base and the negative control, indicating an accelerated wound healing process, while a significant difference was observed on 10th and 14th post –wound days in F.vaillantii treatment compared to the negative control groups. Gel formulation prepared with a 10% F. vaillantii extract exhibited a response in terms of wound epithelialization, angiogenesis and number of hair follicles at wound area better than the gel base on the 21st post-wound day. Application of gel base produced further advantages by increasing hydroxyproline content and collagen fiber thickness. Our results on incision wound model were supported by histopathological data indicating the role of gel base in the enhancement of breaking strength. Conclusion Traditional use of Fumaria species in the skin diseases was justified in this study by revealing the increase in wound healing activity after hydrogel containing F. vaillantii total extract administration. Graphical abstracthttp://link.springer.com/article/10.1186/s12906-019-2645-yWound healingFumaria vaillantiiTopical hydrogelExcisionIncision |
| spellingShingle | Fatemeh Davoodi-Roodbordeii Minoo Afshar Fatemeh Haji Abas Tabrizi Samira Choopani Giti Torkaman Fariborz Moayer Mona Salimi Topical hydrogel containing Fumaria vaillantii Loisel. extract enhances wound healing in rats BMC Complementary and Alternative Medicine Wound healing Fumaria vaillantii Topical hydrogel Excision Incision |
| title | Topical hydrogel containing Fumaria vaillantii Loisel. extract enhances wound healing in rats |
| title_full | Topical hydrogel containing Fumaria vaillantii Loisel. extract enhances wound healing in rats |
| title_fullStr | Topical hydrogel containing Fumaria vaillantii Loisel. extract enhances wound healing in rats |
| title_full_unstemmed | Topical hydrogel containing Fumaria vaillantii Loisel. extract enhances wound healing in rats |
| title_short | Topical hydrogel containing Fumaria vaillantii Loisel. extract enhances wound healing in rats |
| title_sort | topical hydrogel containing fumaria vaillantii loisel extract enhances wound healing in rats |
| topic | Wound healing Fumaria vaillantii Topical hydrogel Excision Incision |
| url | http://link.springer.com/article/10.1186/s12906-019-2645-y |
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