Antibacterial potencial of 12 Lichen species

Abstract Resistant bacterial infections are a major public health problem worldwide, which entails the need to search for new therapeutic agents. In this context, lichens stand out, provided that they are producers of structurally diverse compounds that have attractive biological properties, includi...

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Main Authors: ANA C. MICHELETTI, NELI K. HONDA, LUCIANA M. RAVAGLIA, TATIANA MATAYOSHI, ADRIANO A. SPIELMANN
Format: Article
Language:English
Published: Academia Brasileira de Ciências 2021-10-01
Series:Anais da Academia Brasileira de Ciências
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000700904&tlng=en
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author ANA C. MICHELETTI
NELI K. HONDA
LUCIANA M. RAVAGLIA
TATIANA MATAYOSHI
ADRIANO A. SPIELMANN
author_facet ANA C. MICHELETTI
NELI K. HONDA
LUCIANA M. RAVAGLIA
TATIANA MATAYOSHI
ADRIANO A. SPIELMANN
author_sort ANA C. MICHELETTI
collection DOAJ
description Abstract Resistant bacterial infections are a major public health problem worldwide, which entails the need to search for new therapeutic agents. In this context, lichens stand out, provided that they are producers of structurally diverse compounds that have attractive biological properties, including antimicrobial activity. Thus, extracts of 12 lichen species were prepared and their potential to inhibit the growth of 5 bacterial strains was evaluated in this work. The chemical compositions of these extracts were examined using TLC and microcrystallization, being the identity of the active compounds in each extract attributed based on the bioautography technique. The most active extracts (and their identified active compounds) were from Cladonia borealis (usnic, barbatic and 4-O-demethylbarbatic acids), Cladina confusa (usnic and perlatolic acids), Stereocaulom ramulosum (atranorin, perlatolic and anziaic acids) and Canoparmelia cryptochlorophaea (cryptochlorophaeic and caperatic acids), with MICs ranging from 7.8 to 31.25 μg/mL, including for resistant clinical strains. MIC values were also obtained for substances isolated from lichens for comparison purposes. A group of four extracts containing usnic acid was analyzed by 1H NMR in order to correlate relative proportion of major metabolites and extracts activity. The less active extracts in this group, in fact, presented low proportion of usnic acid.
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spelling doaj.art-26fc2a8f057c470880bc5f38d9d546072022-12-21T17:33:41ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências1678-26902021-10-0193410.1590/0001-3765202120191194Antibacterial potencial of 12 Lichen speciesANA C. MICHELETTIhttps://orcid.org/0000-0002-0797-6669NELI K. HONDAhttps://orcid.org/0000-0001-9417-4885LUCIANA M. RAVAGLIAhttps://orcid.org/0000-0001-7546-0229TATIANA MATAYOSHIhttps://orcid.org/0000-0002-1678-5776ADRIANO A. SPIELMANNhttps://orcid.org/0000-0003-0137-0807Abstract Resistant bacterial infections are a major public health problem worldwide, which entails the need to search for new therapeutic agents. In this context, lichens stand out, provided that they are producers of structurally diverse compounds that have attractive biological properties, including antimicrobial activity. Thus, extracts of 12 lichen species were prepared and their potential to inhibit the growth of 5 bacterial strains was evaluated in this work. The chemical compositions of these extracts were examined using TLC and microcrystallization, being the identity of the active compounds in each extract attributed based on the bioautography technique. The most active extracts (and their identified active compounds) were from Cladonia borealis (usnic, barbatic and 4-O-demethylbarbatic acids), Cladina confusa (usnic and perlatolic acids), Stereocaulom ramulosum (atranorin, perlatolic and anziaic acids) and Canoparmelia cryptochlorophaea (cryptochlorophaeic and caperatic acids), with MICs ranging from 7.8 to 31.25 μg/mL, including for resistant clinical strains. MIC values were also obtained for substances isolated from lichens for comparison purposes. A group of four extracts containing usnic acid was analyzed by 1H NMR in order to correlate relative proportion of major metabolites and extracts activity. The less active extracts in this group, in fact, presented low proportion of usnic acid.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000700904&tlng=enAntimicrobialbioautographylichenmicrodilution
spellingShingle ANA C. MICHELETTI
NELI K. HONDA
LUCIANA M. RAVAGLIA
TATIANA MATAYOSHI
ADRIANO A. SPIELMANN
Antibacterial potencial of 12 Lichen species
Anais da Academia Brasileira de Ciências
Antimicrobial
bioautography
lichen
microdilution
title Antibacterial potencial of 12 Lichen species
title_full Antibacterial potencial of 12 Lichen species
title_fullStr Antibacterial potencial of 12 Lichen species
title_full_unstemmed Antibacterial potencial of 12 Lichen species
title_short Antibacterial potencial of 12 Lichen species
title_sort antibacterial potencial of 12 lichen species
topic Antimicrobial
bioautography
lichen
microdilution
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000700904&tlng=en
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