Distinct skeletal muscle fiber characteristics and gene expression in diet-sensitive versus diet-resistant obesity
Inter-individual variability in weight gain and loss under energy surfeit and deficit conditions, respectively, are well recognized but poorly understood phenomena. We documented weight loss variability in an intensively supervised clinical weight loss program and assessed skeletal muscle gene expre...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2010-08-01
|
| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520370759 |
| _version_ | 1818920415921176576 |
|---|---|
| author | Martin F. Gerrits Sujoy Ghosh Nihan Kavaslar Benjamin Hill Anastasia Tour Erin L. Seifert Brittany Beauchamp Shelby Gorman Joan Stuart Robert Dent Ruth McPherson Mary-Ellen Harper |
| author_facet | Martin F. Gerrits Sujoy Ghosh Nihan Kavaslar Benjamin Hill Anastasia Tour Erin L. Seifert Brittany Beauchamp Shelby Gorman Joan Stuart Robert Dent Ruth McPherson Mary-Ellen Harper |
| author_sort | Martin F. Gerrits |
| collection | DOAJ |
| description | Inter-individual variability in weight gain and loss under energy surfeit and deficit conditions, respectively, are well recognized but poorly understood phenomena. We documented weight loss variability in an intensively supervised clinical weight loss program and assessed skeletal muscle gene expression and phenotypic characteristics related to variable response to a 900 kcal regimen. Matched pairs of healthy, diet-compliant, obese diet-sensitive (ODS) and diet-resistant (ODR) subjects were defined as those in the highest and lowest quintiles for weight loss rate. Physical activity energy expenditure was minimal and comparable. Following program completion and weight stabilization, skeletal muscle biopsies were obtained. Gene expression analysis of rectus femoris and vastus lateralis indicated upregulation of genes and gene sets involved in oxidative phosphorylation and glucose and fatty acid metabolism in ODS compared with ODR. In vastus lateralis, there was a higher proportion of oxidative (type I) fibers in ODS compared with ODR women and lean controls, fiber hypertrophy in ODS compared with ODR women and lean controls, and lower succinate dehydrogenase in oxidative and oxidative-glycolytic fibers in all obese compared with lean subjects. Intramuscular lipid content was generally higher in obese versus lean, and specifically higher in ODS vs. lean women. Altogether, our findings demonstrate differences in muscle gene expression and fiber composition related to clinical weight loss success. |
| first_indexed | 2024-12-20T01:21:24Z |
| format | Article |
| id | doaj.art-26fc79bb8a5248e388f5d32acb63e61e |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-20T01:21:24Z |
| publishDate | 2010-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-26fc79bb8a5248e388f5d32acb63e61e2022-12-21T19:58:24ZengElsevierJournal of Lipid Research0022-22752010-08-0151823942404Distinct skeletal muscle fiber characteristics and gene expression in diet-sensitive versus diet-resistant obesityMartin F. Gerrits0Sujoy Ghosh1Nihan Kavaslar2Benjamin Hill3Anastasia Tour4Erin L. Seifert5Brittany Beauchamp6Shelby Gorman7Joan Stuart8Robert Dent9Ruth McPherson10Mary-Ellen Harper11Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, 451 Smyth Rd., Ottawa, ON, Canada K1H 8M5Biomedicine Biotechnology Research Institute, North Carolina Central University, Durham, NC 27707University of Ottawa Heart Institute, 40 Ruskin Avenue, Ottawa, ON, Canada K1Y 4W7Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, 451 Smyth Rd., Ottawa, ON, Canada K1H 8M5Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, 451 Smyth Rd., Ottawa, ON, Canada K1H 8M5Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, 451 Smyth Rd., Ottawa, ON, Canada K1H 8M5Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, 451 Smyth Rd., Ottawa, ON, Canada K1H 8M5GlaxoSmithKline, R&D, 5 Moore Drive, RTP, NC 27709GlaxoSmithKline, R&D, 5 Moore Drive, RTP, NC 27709Ottawa Hospital Weight Management Clinic, Ottawa Hospital, 1053 Carling Avenue, Ottawa, ON, Canada K1Y 4E9To whom correspondence should be addressed.; University of Ottawa Heart Institute, 40 Ruskin Avenue, Ottawa, ON, Canada K1Y 4W7To whom correspondence should be addressed.; Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, 451 Smyth Rd., Ottawa, ON, Canada K1H 8M5Inter-individual variability in weight gain and loss under energy surfeit and deficit conditions, respectively, are well recognized but poorly understood phenomena. We documented weight loss variability in an intensively supervised clinical weight loss program and assessed skeletal muscle gene expression and phenotypic characteristics related to variable response to a 900 kcal regimen. Matched pairs of healthy, diet-compliant, obese diet-sensitive (ODS) and diet-resistant (ODR) subjects were defined as those in the highest and lowest quintiles for weight loss rate. Physical activity energy expenditure was minimal and comparable. Following program completion and weight stabilization, skeletal muscle biopsies were obtained. Gene expression analysis of rectus femoris and vastus lateralis indicated upregulation of genes and gene sets involved in oxidative phosphorylation and glucose and fatty acid metabolism in ODS compared with ODR. In vastus lateralis, there was a higher proportion of oxidative (type I) fibers in ODS compared with ODR women and lean controls, fiber hypertrophy in ODS compared with ODR women and lean controls, and lower succinate dehydrogenase in oxidative and oxidative-glycolytic fibers in all obese compared with lean subjects. Intramuscular lipid content was generally higher in obese versus lean, and specifically higher in ODS vs. lean women. Altogether, our findings demonstrate differences in muscle gene expression and fiber composition related to clinical weight loss success.http://www.sciencedirect.com/science/article/pii/S0022227520370759mitochondriaoxidative phosphorylationfiber typethermogenesis |
| spellingShingle | Martin F. Gerrits Sujoy Ghosh Nihan Kavaslar Benjamin Hill Anastasia Tour Erin L. Seifert Brittany Beauchamp Shelby Gorman Joan Stuart Robert Dent Ruth McPherson Mary-Ellen Harper Distinct skeletal muscle fiber characteristics and gene expression in diet-sensitive versus diet-resistant obesity Journal of Lipid Research mitochondria oxidative phosphorylation fiber type thermogenesis |
| title | Distinct skeletal muscle fiber characteristics and gene expression in diet-sensitive versus diet-resistant obesity |
| title_full | Distinct skeletal muscle fiber characteristics and gene expression in diet-sensitive versus diet-resistant obesity |
| title_fullStr | Distinct skeletal muscle fiber characteristics and gene expression in diet-sensitive versus diet-resistant obesity |
| title_full_unstemmed | Distinct skeletal muscle fiber characteristics and gene expression in diet-sensitive versus diet-resistant obesity |
| title_short | Distinct skeletal muscle fiber characteristics and gene expression in diet-sensitive versus diet-resistant obesity |
| title_sort | distinct skeletal muscle fiber characteristics and gene expression in diet sensitive versus diet resistant obesity |
| topic | mitochondria oxidative phosphorylation fiber type thermogenesis |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520370759 |
| work_keys_str_mv | AT martinfgerrits distinctskeletalmusclefibercharacteristicsandgeneexpressionindietsensitiveversusdietresistantobesity AT sujoyghosh distinctskeletalmusclefibercharacteristicsandgeneexpressionindietsensitiveversusdietresistantobesity AT nihankavaslar distinctskeletalmusclefibercharacteristicsandgeneexpressionindietsensitiveversusdietresistantobesity AT benjaminhill distinctskeletalmusclefibercharacteristicsandgeneexpressionindietsensitiveversusdietresistantobesity AT anastasiatour distinctskeletalmusclefibercharacteristicsandgeneexpressionindietsensitiveversusdietresistantobesity AT erinlseifert distinctskeletalmusclefibercharacteristicsandgeneexpressionindietsensitiveversusdietresistantobesity AT brittanybeauchamp distinctskeletalmusclefibercharacteristicsandgeneexpressionindietsensitiveversusdietresistantobesity AT shelbygorman distinctskeletalmusclefibercharacteristicsandgeneexpressionindietsensitiveversusdietresistantobesity AT joanstuart distinctskeletalmusclefibercharacteristicsandgeneexpressionindietsensitiveversusdietresistantobesity AT robertdent distinctskeletalmusclefibercharacteristicsandgeneexpressionindietsensitiveversusdietresistantobesity AT ruthmcpherson distinctskeletalmusclefibercharacteristicsandgeneexpressionindietsensitiveversusdietresistantobesity AT maryellenharper distinctskeletalmusclefibercharacteristicsandgeneexpressionindietsensitiveversusdietresistantobesity |