Prevalence and Antibiotic Resistance of <i>Salmonella</i> and <i>Campylobacter</i> Isolates from Raw Chicken Breasts in Retail Markets in the United States and Comparison to Data from the Plant Level
Chicken is the most popular meat in the United States, and consumers may be exposed to multidrug resistant <i>Salmonella</i> and <i>Campylobacter</i> through consumption of retail chicken breasts. This study aimed to (i) determine the percentage of raw, packaged, retail chick...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-02-01
|
| Series: | Life |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-1729/13/3/642 |
| _version_ | 1797610635472666624 |
|---|---|
| author | Sana Mujahid Michael Hansen Robyn Miranda Keith Newsom-Stewart James E. Rogers |
| author_facet | Sana Mujahid Michael Hansen Robyn Miranda Keith Newsom-Stewart James E. Rogers |
| author_sort | Sana Mujahid |
| collection | DOAJ |
| description | Chicken is the most popular meat in the United States, and consumers may be exposed to multidrug resistant <i>Salmonella</i> and <i>Campylobacter</i> through consumption of retail chicken breasts. This study aimed to (i) determine the percentage of raw, packaged, retail chicken breasts from 27 metro areas that tested positive for <i>Salmonella</i> and <i>Campylobacter</i>; (ii) investigate the antibiotic susceptibility profiles of a subset of the isolates; and (iii) compare the <i>Salmonella</i> prevalence data to establishment level <i>Salmonella</i> categorization data published by the U.S. Department of Agriculture (USDA). USDA Food Safety and Inspection Service (FSIS) Microbiology Laboratory Guidebook (MLG) methodology was used to isolate and identify <i>Salmonella</i> (<i>n</i> = 672), <i>Campylobacter</i> (<i>n</i> = 499) from 400 g samples. National Antimicrobial Resistance Monitoring System (NARMS) methodology was followed for antimicrobial susceptibility testing of <i>Salmonella</i> (<i>n</i> = 52) and <i>Campylobacter</i> (<i>n</i> = 16) isolates. <i>Salmonella</i> was found in 8.6% of samples and <i>Campylobacter</i> in 4.2%. Having a 3 rating in USDA’s <i>Salmonella</i> Categorization of Individual Establishments for chicken parts was predictive of having a higher <i>Salmonella</i> percent positive in our data set (<i>p</i> ≤ 0.05). A total of 73.1% of <i>Salmonella</i> isolates, and 62.5% of <i>Campylobacter</i> isolates were resistant to ≥one class of antibiotics, with 48.1% of <i>Salmonella</i> isolates resistant to ≥three classes. Current results support interventions that take a ‘farm-to-fork’ approach with distinction by poultry types and parts as well as serovars, to lower antibiotic resistant <i>Salmonella</i> infections in humans due to poultry. <b>Highlights:</b> <i>Salmonella</i> was found in 8.6% and <i>Campylobacter</i> in 4.2% of chicken breasts tested; A 3 rating by USDA was predictive of a higher <i>Salmonella</i> percent positive; 48.1% of <i>Salmonella</i> isolates were resistant to 3 or more classes of antibiotics. |
| first_indexed | 2024-03-11T06:16:58Z |
| format | Article |
| id | doaj.art-26fd882dfcc04a7b8778ed6e881a27bd |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-11T06:16:58Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-26fd882dfcc04a7b8778ed6e881a27bd2023-11-17T12:10:21ZengMDPI AGLife2075-17292023-02-0113364210.3390/life13030642Prevalence and Antibiotic Resistance of <i>Salmonella</i> and <i>Campylobacter</i> Isolates from Raw Chicken Breasts in Retail Markets in the United States and Comparison to Data from the Plant LevelSana Mujahid0Michael Hansen1Robyn Miranda2Keith Newsom-Stewart3James E. Rogers4Consumer Reports, 101 Truman Avenue, Yonkers, NY 10703, USAConsumer Reports, 101 Truman Avenue, Yonkers, NY 10703, USAConsumer Reports, 101 Truman Avenue, Yonkers, NY 10703, USAConsumer Reports, 101 Truman Avenue, Yonkers, NY 10703, USAConsumer Reports, 101 Truman Avenue, Yonkers, NY 10703, USAChicken is the most popular meat in the United States, and consumers may be exposed to multidrug resistant <i>Salmonella</i> and <i>Campylobacter</i> through consumption of retail chicken breasts. This study aimed to (i) determine the percentage of raw, packaged, retail chicken breasts from 27 metro areas that tested positive for <i>Salmonella</i> and <i>Campylobacter</i>; (ii) investigate the antibiotic susceptibility profiles of a subset of the isolates; and (iii) compare the <i>Salmonella</i> prevalence data to establishment level <i>Salmonella</i> categorization data published by the U.S. Department of Agriculture (USDA). USDA Food Safety and Inspection Service (FSIS) Microbiology Laboratory Guidebook (MLG) methodology was used to isolate and identify <i>Salmonella</i> (<i>n</i> = 672), <i>Campylobacter</i> (<i>n</i> = 499) from 400 g samples. National Antimicrobial Resistance Monitoring System (NARMS) methodology was followed for antimicrobial susceptibility testing of <i>Salmonella</i> (<i>n</i> = 52) and <i>Campylobacter</i> (<i>n</i> = 16) isolates. <i>Salmonella</i> was found in 8.6% of samples and <i>Campylobacter</i> in 4.2%. Having a 3 rating in USDA’s <i>Salmonella</i> Categorization of Individual Establishments for chicken parts was predictive of having a higher <i>Salmonella</i> percent positive in our data set (<i>p</i> ≤ 0.05). A total of 73.1% of <i>Salmonella</i> isolates, and 62.5% of <i>Campylobacter</i> isolates were resistant to ≥one class of antibiotics, with 48.1% of <i>Salmonella</i> isolates resistant to ≥three classes. Current results support interventions that take a ‘farm-to-fork’ approach with distinction by poultry types and parts as well as serovars, to lower antibiotic resistant <i>Salmonella</i> infections in humans due to poultry. <b>Highlights:</b> <i>Salmonella</i> was found in 8.6% and <i>Campylobacter</i> in 4.2% of chicken breasts tested; A 3 rating by USDA was predictive of a higher <i>Salmonella</i> percent positive; 48.1% of <i>Salmonella</i> isolates were resistant to 3 or more classes of antibiotics.https://www.mdpi.com/2075-1729/13/3/642<i>Salmonella</i><i>Campylobacter</i>antibiotic resistanceretail chicken |
| spellingShingle | Sana Mujahid Michael Hansen Robyn Miranda Keith Newsom-Stewart James E. Rogers Prevalence and Antibiotic Resistance of <i>Salmonella</i> and <i>Campylobacter</i> Isolates from Raw Chicken Breasts in Retail Markets in the United States and Comparison to Data from the Plant Level Life <i>Salmonella</i> <i>Campylobacter</i> antibiotic resistance retail chicken |
| title | Prevalence and Antibiotic Resistance of <i>Salmonella</i> and <i>Campylobacter</i> Isolates from Raw Chicken Breasts in Retail Markets in the United States and Comparison to Data from the Plant Level |
| title_full | Prevalence and Antibiotic Resistance of <i>Salmonella</i> and <i>Campylobacter</i> Isolates from Raw Chicken Breasts in Retail Markets in the United States and Comparison to Data from the Plant Level |
| title_fullStr | Prevalence and Antibiotic Resistance of <i>Salmonella</i> and <i>Campylobacter</i> Isolates from Raw Chicken Breasts in Retail Markets in the United States and Comparison to Data from the Plant Level |
| title_full_unstemmed | Prevalence and Antibiotic Resistance of <i>Salmonella</i> and <i>Campylobacter</i> Isolates from Raw Chicken Breasts in Retail Markets in the United States and Comparison to Data from the Plant Level |
| title_short | Prevalence and Antibiotic Resistance of <i>Salmonella</i> and <i>Campylobacter</i> Isolates from Raw Chicken Breasts in Retail Markets in the United States and Comparison to Data from the Plant Level |
| title_sort | prevalence and antibiotic resistance of i salmonella i and i campylobacter i isolates from raw chicken breasts in retail markets in the united states and comparison to data from the plant level |
| topic | <i>Salmonella</i> <i>Campylobacter</i> antibiotic resistance retail chicken |
| url | https://www.mdpi.com/2075-1729/13/3/642 |
| work_keys_str_mv | AT sanamujahid prevalenceandantibioticresistanceofisalmonellaiandicampylobacteriisolatesfromrawchickenbreastsinretailmarketsintheunitedstatesandcomparisontodatafromtheplantlevel AT michaelhansen prevalenceandantibioticresistanceofisalmonellaiandicampylobacteriisolatesfromrawchickenbreastsinretailmarketsintheunitedstatesandcomparisontodatafromtheplantlevel AT robynmiranda prevalenceandantibioticresistanceofisalmonellaiandicampylobacteriisolatesfromrawchickenbreastsinretailmarketsintheunitedstatesandcomparisontodatafromtheplantlevel AT keithnewsomstewart prevalenceandantibioticresistanceofisalmonellaiandicampylobacteriisolatesfromrawchickenbreastsinretailmarketsintheunitedstatesandcomparisontodatafromtheplantlevel AT jameserogers prevalenceandantibioticresistanceofisalmonellaiandicampylobacteriisolatesfromrawchickenbreastsinretailmarketsintheunitedstatesandcomparisontodatafromtheplantlevel |