Protective effects of Camellia sinensis on Syzygium aromaticum- or chlorpyrifos-induced reproductive toxicity in male Wistar rats
Abstract Background The potential toxicity of clove, Syzygium aromaticum, notwithstanding its beneficial health effect to human health remains a critical issue. Purpose This study was designed to assess the effects of oil extracts of clove (S. aromaticum) on reproductive parameters in Wistar rats. T...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2019-07-01
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| Series: | Journal of Basic and Applied Zoology |
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| Online Access: | http://link.springer.com/article/10.1186/s41936-019-0122-2 |
| _version_ | 1811273597525688320 |
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| author | Damola V. Akinwande Joseph A. Adeyemi Solomon T. Olawuyi Busuyi K. Akinola Chris O. Adedire |
| author_facet | Damola V. Akinwande Joseph A. Adeyemi Solomon T. Olawuyi Busuyi K. Akinola Chris O. Adedire |
| author_sort | Damola V. Akinwande |
| collection | DOAJ |
| description | Abstract Background The potential toxicity of clove, Syzygium aromaticum, notwithstanding its beneficial health effect to human health remains a critical issue. Purpose This study was designed to assess the effects of oil extracts of clove (S. aromaticum) on reproductive parameters in Wistar rats. The ameliorative effect due to co-administration with green tea, Camellia sinensis, was also determined. Methods Adult rats were exposed via oral gavage to mineral oil (negative control), 5% green tea (GT), 12.5 mg/kg/day chlorpyrifos (CHL, positive control), 360 mg/kg/day clove oil (CO), green tea + chlorpyrifos (GT+CHL), or green tea + clove oil (GT+CO) for 3 weeks, after which the animals were sacrificed and the following sperm parameters: total sperm count, sperm motile count, sperm progressive assessment, and sperm morphology were determined. The serum levels of reproductive hormones, testosterone, follicle stimulating hormone (FSH) and luteinizing hormone (LH), were determined. The histological sections of the testes were also performed. Results The results revealed that S. aromaticum treatment resulted in significant damage to the sperm morphology especially at the neck and tail regions with only a marginal change to the total sperm count, sperm motile count, and sperm progressive assessment. The levels of testosterone, FSH, and LH decreased significantly in rats treated with S. aromaticum. Histopathological analyses revealed significant disruption of normal testes structure in rats that were treated with either clove oil or chlorpyrifos. Conclusion Overall, the results of this study show that the co-administration with C. sinensis has the potential to ameliorate the clove-induced reproductive toxicity in rats. |
| first_indexed | 2024-04-12T23:02:07Z |
| format | Article |
| id | doaj.art-26fe8bf843b944c191fddfae77fbf829 |
| institution | Directory Open Access Journal |
| issn | 2090-990X |
| language | English |
| last_indexed | 2024-04-12T23:02:07Z |
| publishDate | 2019-07-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Journal of Basic and Applied Zoology |
| spelling | doaj.art-26fe8bf843b944c191fddfae77fbf8292022-12-22T03:13:01ZengSpringerOpenJournal of Basic and Applied Zoology2090-990X2019-07-018011910.1186/s41936-019-0122-2Protective effects of Camellia sinensis on Syzygium aromaticum- or chlorpyrifos-induced reproductive toxicity in male Wistar ratsDamola V. Akinwande0Joseph A. Adeyemi1Solomon T. Olawuyi2Busuyi K. Akinola3Chris O. Adedire4Department of Biology, School of Sciences, Federal University of TechnologyDepartment of Biology, School of Sciences, Federal University of TechnologyDepartment of Anatomy, School of Health Sciences, Federal University of TechnologyDepartment of Anatomy, School of Health Sciences, Federal University of TechnologyDepartment of Biology, School of Sciences, Federal University of TechnologyAbstract Background The potential toxicity of clove, Syzygium aromaticum, notwithstanding its beneficial health effect to human health remains a critical issue. Purpose This study was designed to assess the effects of oil extracts of clove (S. aromaticum) on reproductive parameters in Wistar rats. The ameliorative effect due to co-administration with green tea, Camellia sinensis, was also determined. Methods Adult rats were exposed via oral gavage to mineral oil (negative control), 5% green tea (GT), 12.5 mg/kg/day chlorpyrifos (CHL, positive control), 360 mg/kg/day clove oil (CO), green tea + chlorpyrifos (GT+CHL), or green tea + clove oil (GT+CO) for 3 weeks, after which the animals were sacrificed and the following sperm parameters: total sperm count, sperm motile count, sperm progressive assessment, and sperm morphology were determined. The serum levels of reproductive hormones, testosterone, follicle stimulating hormone (FSH) and luteinizing hormone (LH), were determined. The histological sections of the testes were also performed. Results The results revealed that S. aromaticum treatment resulted in significant damage to the sperm morphology especially at the neck and tail regions with only a marginal change to the total sperm count, sperm motile count, and sperm progressive assessment. The levels of testosterone, FSH, and LH decreased significantly in rats treated with S. aromaticum. Histopathological analyses revealed significant disruption of normal testes structure in rats that were treated with either clove oil or chlorpyrifos. Conclusion Overall, the results of this study show that the co-administration with C. sinensis has the potential to ameliorate the clove-induced reproductive toxicity in rats.http://link.springer.com/article/10.1186/s41936-019-0122-2Plant-based pesticideChlorpyrifosSyzygium aromaticumSperm parametersSex hormonesHistopathology |
| spellingShingle | Damola V. Akinwande Joseph A. Adeyemi Solomon T. Olawuyi Busuyi K. Akinola Chris O. Adedire Protective effects of Camellia sinensis on Syzygium aromaticum- or chlorpyrifos-induced reproductive toxicity in male Wistar rats Journal of Basic and Applied Zoology Plant-based pesticide Chlorpyrifos Syzygium aromaticum Sperm parameters Sex hormones Histopathology |
| title | Protective effects of Camellia sinensis on Syzygium aromaticum- or chlorpyrifos-induced reproductive toxicity in male Wistar rats |
| title_full | Protective effects of Camellia sinensis on Syzygium aromaticum- or chlorpyrifos-induced reproductive toxicity in male Wistar rats |
| title_fullStr | Protective effects of Camellia sinensis on Syzygium aromaticum- or chlorpyrifos-induced reproductive toxicity in male Wistar rats |
| title_full_unstemmed | Protective effects of Camellia sinensis on Syzygium aromaticum- or chlorpyrifos-induced reproductive toxicity in male Wistar rats |
| title_short | Protective effects of Camellia sinensis on Syzygium aromaticum- or chlorpyrifos-induced reproductive toxicity in male Wistar rats |
| title_sort | protective effects of camellia sinensis on syzygium aromaticum or chlorpyrifos induced reproductive toxicity in male wistar rats |
| topic | Plant-based pesticide Chlorpyrifos Syzygium aromaticum Sperm parameters Sex hormones Histopathology |
| url | http://link.springer.com/article/10.1186/s41936-019-0122-2 |
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