Transcriptional Inhibition of <i>Sp-IAG</i> by Crustacean Female Sex Hormone in the Mud Crab, <i>Scylla paramamosain</i>
In crustaceans, the regulation of sex differentiation is mediated by insulin-like androgenic hormone (IAG) and crustacean female sex hormone (CFSH). CFSH is reported to inhibit IAG gene (<i>Sp-IAG</i>) expression in the mud crab <i>Scylla paramamosain</i>, but the regulatory...
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MDPI AG
2020-07-01
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| Series: | International Journal of Molecular Sciences |
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| author | Qingling Jiang Bei Lu Guizhong Wang Haihui Ye |
| author_facet | Qingling Jiang Bei Lu Guizhong Wang Haihui Ye |
| author_sort | Qingling Jiang |
| collection | DOAJ |
| description | In crustaceans, the regulation of sex differentiation is mediated by insulin-like androgenic hormone (IAG) and crustacean female sex hormone (CFSH). CFSH is reported to inhibit IAG gene (<i>Sp-IAG</i>) expression in the mud crab <i>Scylla paramamosain</i>, but the regulatory mechanism is not well understood. A 2674 bp 5′ flanking <i>Sp-IAG</i> contains many potential transcription factor binding sites. In this study, analysis of serially deleted 5′ flanking <i>Sp-IAG</i> and site-directed mutation (SDM) of transcription factor binding sites of the same gene showed that the promoter activity of reporter vectors with Sox-5-binding site, signal transducers and activators of transcription (STAT)-binding site and activator protein 1 (AP-1)-binding site were significantly higher than that of vectors without these regions, suggesting that they were involved in transcriptional regulation of <i>Sp-IAG</i> expression. The expression analysis of these transcription factor showed that there was no difference in the level of mRNA in <i>Sox-5</i> and <i>AP-1</i> in androgenic gland treated with recombinant CFSH, but expression of <i>Sp-STAT</i> was significantly reduced, suggesting that CFSH regulates the expression of <i>Sp-STAT</i>, inhibiting its function to regulate <i>Sp-IAG</i>. Further experiment revealed that RNAi mediated <i>Sp-STAT</i> gene knockdown reduced the expression of <i>Sp-IAG</i>. These results suggested that <i>Sp-</i>CFSH regulates <i>Sp-IAG</i> by inhibiting STAT. This is a pioneering finding on the transcriptional mechanism of <i>IAG</i> gene in crustaceans. |
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| language | English |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-26ffdf88bbf146678c0fa83d65c06fb92023-11-20T08:00:13ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-07-012115530010.3390/ijms21155300Transcriptional Inhibition of <i>Sp-IAG</i> by Crustacean Female Sex Hormone in the Mud Crab, <i>Scylla paramamosain</i>Qingling Jiang0Bei Lu1Guizhong Wang2Haihui Ye3College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, ChinaCollege of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, ChinaCollege of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, ChinaCollege of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, ChinaIn crustaceans, the regulation of sex differentiation is mediated by insulin-like androgenic hormone (IAG) and crustacean female sex hormone (CFSH). CFSH is reported to inhibit IAG gene (<i>Sp-IAG</i>) expression in the mud crab <i>Scylla paramamosain</i>, but the regulatory mechanism is not well understood. A 2674 bp 5′ flanking <i>Sp-IAG</i> contains many potential transcription factor binding sites. In this study, analysis of serially deleted 5′ flanking <i>Sp-IAG</i> and site-directed mutation (SDM) of transcription factor binding sites of the same gene showed that the promoter activity of reporter vectors with Sox-5-binding site, signal transducers and activators of transcription (STAT)-binding site and activator protein 1 (AP-1)-binding site were significantly higher than that of vectors without these regions, suggesting that they were involved in transcriptional regulation of <i>Sp-IAG</i> expression. The expression analysis of these transcription factor showed that there was no difference in the level of mRNA in <i>Sox-5</i> and <i>AP-1</i> in androgenic gland treated with recombinant CFSH, but expression of <i>Sp-STAT</i> was significantly reduced, suggesting that CFSH regulates the expression of <i>Sp-STAT</i>, inhibiting its function to regulate <i>Sp-IAG</i>. Further experiment revealed that RNAi mediated <i>Sp-STAT</i> gene knockdown reduced the expression of <i>Sp-IAG</i>. These results suggested that <i>Sp-</i>CFSH regulates <i>Sp-IAG</i> by inhibiting STAT. This is a pioneering finding on the transcriptional mechanism of <i>IAG</i> gene in crustaceans.https://www.mdpi.com/1422-0067/21/15/5300insulin-like androgenic hormonetranscription regulationcrustacean female sex hormonecrustacean |
| spellingShingle | Qingling Jiang Bei Lu Guizhong Wang Haihui Ye Transcriptional Inhibition of <i>Sp-IAG</i> by Crustacean Female Sex Hormone in the Mud Crab, <i>Scylla paramamosain</i> International Journal of Molecular Sciences insulin-like androgenic hormone transcription regulation crustacean female sex hormone crustacean |
| title | Transcriptional Inhibition of <i>Sp-IAG</i> by Crustacean Female Sex Hormone in the Mud Crab, <i>Scylla paramamosain</i> |
| title_full | Transcriptional Inhibition of <i>Sp-IAG</i> by Crustacean Female Sex Hormone in the Mud Crab, <i>Scylla paramamosain</i> |
| title_fullStr | Transcriptional Inhibition of <i>Sp-IAG</i> by Crustacean Female Sex Hormone in the Mud Crab, <i>Scylla paramamosain</i> |
| title_full_unstemmed | Transcriptional Inhibition of <i>Sp-IAG</i> by Crustacean Female Sex Hormone in the Mud Crab, <i>Scylla paramamosain</i> |
| title_short | Transcriptional Inhibition of <i>Sp-IAG</i> by Crustacean Female Sex Hormone in the Mud Crab, <i>Scylla paramamosain</i> |
| title_sort | transcriptional inhibition of i sp iag i by crustacean female sex hormone in the mud crab i scylla paramamosain i |
| topic | insulin-like androgenic hormone transcription regulation crustacean female sex hormone crustacean |
| url | https://www.mdpi.com/1422-0067/21/15/5300 |
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