Guidelines and Considerations for Metabolic Tolerance Tests in Mice
Raquel Benedé-Ubieto,1,* Olga Estévez-Vázquez,1,* Pierluigi Ramadori,2 Francisco Javier Cubero,3,4 Yulia A Nevzorova1,4,5 1Department of Physiology, Genetics and Microbiology, Faculty of Biology, Complutense University, Madrid, Spain; 2Division of Chronic Inflammatio...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2020-02-01
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| Series: | Diabetes, Metabolic Syndrome and Obesity |
| Subjects: | |
| Online Access: | https://www.dovepress.com/guidelines-and-considerations-for-metabolic-tolerance-tests-in-mice-peer-reviewed-article-DMSO |
| _version_ | 1797935600598253568 |
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| author | Benedé-Ubieto R Estévez-Vázquez O Ramadori P Cubero FJ Nevzorova YA |
| author_facet | Benedé-Ubieto R Estévez-Vázquez O Ramadori P Cubero FJ Nevzorova YA |
| author_sort | Benedé-Ubieto R |
| collection | DOAJ |
| description | Raquel Benedé-Ubieto,1,* Olga Estévez-Vázquez,1,* Pierluigi Ramadori,2 Francisco Javier Cubero,3,4 Yulia A Nevzorova1,4,5 1Department of Physiology, Genetics and Microbiology, Faculty of Biology, Complutense University, Madrid, Spain; 2Division of Chronic Inflammation and Cancer, German Cancer Research Center Heidelberg (DKFZ), Heidelberg, Germany; 3Department of Immunology, Ophthalmology & ENT, Complutense University School of Medicine, Madrid, Spain; 4 12 de Octubre Health Research Institute (imas12), Madrid, Spain; 5Department of Internal Medicine III, University Hospital RWTH Aachen, Aachen, Germany*These authors contributed equally to this workCorrespondence: Yulia A NevzorovaDepartment of Physiology, Genetics and Microbiology, Faculty of Biology, Complutense University, c/José A. Novais, 2, Madrid 28040, SpainTel +49-(0)241-80-80662Fax +49-(0)241-80-82455Email ynevzorova@ukaachen.deAbstract: The epidemic of the century, Diabetes Mellitus (DM) is continuously rising. Intensive research is urgently needed whereby experimental models represent an essential tool to optimise the diagnostic strategy and to improve therapy. In this review, we describe the central principles of the metabolic tests available in order to study glucose and insulin homeostasis in mice, focusing on the most widely used – the glucose and insulin tolerance tests. We provide detailed experimental procedures as well as the practical implementation of these methods and discuss the main factors that should be taken into account when using this methodology.Keywords: metabolic tolerance test, T2DM, obesity, metabolic syndrome, insulin resistance, glucose intolerance |
| first_indexed | 2024-04-10T18:16:37Z |
| format | Article |
| id | doaj.art-2700436fe7f04a97bb83321911aafcdd |
| institution | Directory Open Access Journal |
| issn | 1178-7007 |
| language | English |
| last_indexed | 2024-04-10T18:16:37Z |
| publishDate | 2020-02-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Diabetes, Metabolic Syndrome and Obesity |
| spelling | doaj.art-2700436fe7f04a97bb83321911aafcdd2023-02-02T08:31:43ZengDove Medical PressDiabetes, Metabolic Syndrome and Obesity1178-70072020-02-01Volume 1343945051883Guidelines and Considerations for Metabolic Tolerance Tests in MiceBenedé-Ubieto REstévez-Vázquez ORamadori PCubero FJNevzorova YARaquel Benedé-Ubieto,1,* Olga Estévez-Vázquez,1,* Pierluigi Ramadori,2 Francisco Javier Cubero,3,4 Yulia A Nevzorova1,4,5 1Department of Physiology, Genetics and Microbiology, Faculty of Biology, Complutense University, Madrid, Spain; 2Division of Chronic Inflammation and Cancer, German Cancer Research Center Heidelberg (DKFZ), Heidelberg, Germany; 3Department of Immunology, Ophthalmology & ENT, Complutense University School of Medicine, Madrid, Spain; 4 12 de Octubre Health Research Institute (imas12), Madrid, Spain; 5Department of Internal Medicine III, University Hospital RWTH Aachen, Aachen, Germany*These authors contributed equally to this workCorrespondence: Yulia A NevzorovaDepartment of Physiology, Genetics and Microbiology, Faculty of Biology, Complutense University, c/José A. Novais, 2, Madrid 28040, SpainTel +49-(0)241-80-80662Fax +49-(0)241-80-82455Email ynevzorova@ukaachen.deAbstract: The epidemic of the century, Diabetes Mellitus (DM) is continuously rising. Intensive research is urgently needed whereby experimental models represent an essential tool to optimise the diagnostic strategy and to improve therapy. In this review, we describe the central principles of the metabolic tests available in order to study glucose and insulin homeostasis in mice, focusing on the most widely used – the glucose and insulin tolerance tests. We provide detailed experimental procedures as well as the practical implementation of these methods and discuss the main factors that should be taken into account when using this methodology.Keywords: metabolic tolerance test, T2DM, obesity, metabolic syndrome, insulin resistance, glucose intolerancehttps://www.dovepress.com/guidelines-and-considerations-for-metabolic-tolerance-tests-in-mice-peer-reviewed-article-DMSOmetabolic tolerance testt2dmobesitymetabolic syndromeinsulin resistanceglucose intolerance. |
| spellingShingle | Benedé-Ubieto R Estévez-Vázquez O Ramadori P Cubero FJ Nevzorova YA Guidelines and Considerations for Metabolic Tolerance Tests in Mice Diabetes, Metabolic Syndrome and Obesity metabolic tolerance test t2dm obesity metabolic syndrome insulin resistance glucose intolerance. |
| title | Guidelines and Considerations for Metabolic Tolerance Tests in Mice |
| title_full | Guidelines and Considerations for Metabolic Tolerance Tests in Mice |
| title_fullStr | Guidelines and Considerations for Metabolic Tolerance Tests in Mice |
| title_full_unstemmed | Guidelines and Considerations for Metabolic Tolerance Tests in Mice |
| title_short | Guidelines and Considerations for Metabolic Tolerance Tests in Mice |
| title_sort | guidelines and considerations for metabolic tolerance tests in mice |
| topic | metabolic tolerance test t2dm obesity metabolic syndrome insulin resistance glucose intolerance. |
| url | https://www.dovepress.com/guidelines-and-considerations-for-metabolic-tolerance-tests-in-mice-peer-reviewed-article-DMSO |
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