Production optimization, stability and oil emulsifying potential of biosurfactants from selected bacteria isolated from oil-contaminated sites
Oil pollution is of increasing concern for environmental safety and the use of microbial surfactants in oil remediation has become inevitable for their efficacy and ecofriendly nature. In this work, biosurfactants of bacteria isolated from oil-contaminated soil have been characterized. Four potent b...
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The Royal Society
2021-10-01
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| Series: | Royal Society Open Science |
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| Online Access: | https://royalsocietypublishing.org/doi/10.1098/rsos.211003 |
| _version_ | 1819018219715821568 |
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| author | Ferdausi Ali Sharup Das Tanim Jabid Hossain Sumaiya Islam Chowdhury Subrina Akter Zedny Tuhin Das Mohammad Nazmul Ahmed Chowdhury Mohammad Seraj Uddin |
| author_facet | Ferdausi Ali Sharup Das Tanim Jabid Hossain Sumaiya Islam Chowdhury Subrina Akter Zedny Tuhin Das Mohammad Nazmul Ahmed Chowdhury Mohammad Seraj Uddin |
| author_sort | Ferdausi Ali |
| collection | DOAJ |
| description | Oil pollution is of increasing concern for environmental safety and the use of microbial surfactants in oil remediation has become inevitable for their efficacy and ecofriendly nature. In this work, biosurfactants of bacteria isolated from oil-contaminated soil have been characterized. Four potent biosurfactant-producing strains (SD4, SD11, SD12 and SD13) were selected from 27 isolates based on drop collapse assay and emulsification index, and identified as species belonging to Bacillus, Burkholderia, Providencia and Klebsiella, revealed from their 16S rRNA gene-based analysis. Detailed morphological and biochemical characteristics of each selected isolate were determined. Their growth conditions for maximum biosurfactant production were optimized and found quite similar among the four isolates with a pH of 3.0 and temperature 37°C after 6 or 7 days of growth on kerosene. The biosurfactants of SD4, SD11 and SD12 appeared to be glycolipids and that of SD13 a lipopeptide. Emulsification activity of most of the biosurfactants was stable at low and high temperatures (4–100°C), a wide range of pH (2–10) and salt concentrations (2–7% NaCl). Each biosurfactant showed antimicrobial activity against two or more pathogenic bacteria. The biosurfactants were well-capable of emulsifying kerosene, diesel and soya bean, and could efficiently degrade diesel. |
| first_indexed | 2024-12-21T03:15:57Z |
| format | Article |
| id | doaj.art-270056f393da4ffca7f5913951ece7c6 |
| institution | Directory Open Access Journal |
| issn | 2054-5703 |
| language | English |
| last_indexed | 2024-12-21T03:15:57Z |
| publishDate | 2021-10-01 |
| publisher | The Royal Society |
| record_format | Article |
| series | Royal Society Open Science |
| spelling | doaj.art-270056f393da4ffca7f5913951ece7c62022-12-21T19:17:50ZengThe Royal SocietyRoyal Society Open Science2054-57032021-10-0181010.1098/rsos.211003Production optimization, stability and oil emulsifying potential of biosurfactants from selected bacteria isolated from oil-contaminated sitesFerdausi Ali0Sharup Das1Tanim Jabid Hossain2Sumaiya Islam Chowdhury3Subrina Akter Zedny4Tuhin Das5Mohammad Nazmul Ahmed Chowdhury6Mohammad Seraj Uddin7Department of Microbiology, University of Chittagong, Chattogram 4331, BangladeshDepartment of Microbiology, University of Chittagong, Chattogram 4331, BangladeshDepartment of Biochemistry and Molecular Biology, University of Chittagong, Chattogram 4331, BangladeshDepartment of Biochemistry and Molecular Biology, University of Chittagong, Chattogram 4331, BangladeshDepartment of Microbiology, University of Chittagong, Chattogram 4331, BangladeshDepartment of Microbiology, University of Chittagong, Chattogram 4331, BangladeshDepartment of Biochemistry and Molecular Biology, University of Chittagong, Chattogram 4331, BangladeshDepartment of Microbiology, University of Chittagong, Chattogram 4331, BangladeshOil pollution is of increasing concern for environmental safety and the use of microbial surfactants in oil remediation has become inevitable for their efficacy and ecofriendly nature. In this work, biosurfactants of bacteria isolated from oil-contaminated soil have been characterized. Four potent biosurfactant-producing strains (SD4, SD11, SD12 and SD13) were selected from 27 isolates based on drop collapse assay and emulsification index, and identified as species belonging to Bacillus, Burkholderia, Providencia and Klebsiella, revealed from their 16S rRNA gene-based analysis. Detailed morphological and biochemical characteristics of each selected isolate were determined. Their growth conditions for maximum biosurfactant production were optimized and found quite similar among the four isolates with a pH of 3.0 and temperature 37°C after 6 or 7 days of growth on kerosene. The biosurfactants of SD4, SD11 and SD12 appeared to be glycolipids and that of SD13 a lipopeptide. Emulsification activity of most of the biosurfactants was stable at low and high temperatures (4–100°C), a wide range of pH (2–10) and salt concentrations (2–7% NaCl). Each biosurfactant showed antimicrobial activity against two or more pathogenic bacteria. The biosurfactants were well-capable of emulsifying kerosene, diesel and soya bean, and could efficiently degrade diesel.https://royalsocietypublishing.org/doi/10.1098/rsos.211003microbial surfactantsbiosurfactant-producing bacteriapetroleum hydrocarbon bioremediationcrude oil biodegradationemulsification indexproduction optimization |
| spellingShingle | Ferdausi Ali Sharup Das Tanim Jabid Hossain Sumaiya Islam Chowdhury Subrina Akter Zedny Tuhin Das Mohammad Nazmul Ahmed Chowdhury Mohammad Seraj Uddin Production optimization, stability and oil emulsifying potential of biosurfactants from selected bacteria isolated from oil-contaminated sites Royal Society Open Science microbial surfactants biosurfactant-producing bacteria petroleum hydrocarbon bioremediation crude oil biodegradation emulsification index production optimization |
| title | Production optimization, stability and oil emulsifying potential of biosurfactants from selected bacteria isolated from oil-contaminated sites |
| title_full | Production optimization, stability and oil emulsifying potential of biosurfactants from selected bacteria isolated from oil-contaminated sites |
| title_fullStr | Production optimization, stability and oil emulsifying potential of biosurfactants from selected bacteria isolated from oil-contaminated sites |
| title_full_unstemmed | Production optimization, stability and oil emulsifying potential of biosurfactants from selected bacteria isolated from oil-contaminated sites |
| title_short | Production optimization, stability and oil emulsifying potential of biosurfactants from selected bacteria isolated from oil-contaminated sites |
| title_sort | production optimization stability and oil emulsifying potential of biosurfactants from selected bacteria isolated from oil contaminated sites |
| topic | microbial surfactants biosurfactant-producing bacteria petroleum hydrocarbon bioremediation crude oil biodegradation emulsification index production optimization |
| url | https://royalsocietypublishing.org/doi/10.1098/rsos.211003 |
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