Histomorphometric and immunohistochemical evaluation of collagen containing xenogeneic bone blocks used for lateral bone augmentation in staged implant placement
Abstract Background The osteoconductive properties of collagen containing xenogeneic bone blocks (CCXBB) remain unclear. The aim of this prospective single-arm clinical study was to assess the histological outcomes of CCXBB blocks used as bone replacement grafts for lateral bone augmentation procedu...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2017-06-01
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| Series: | International Journal of Implant Dentistry |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s40729-017-0087-1 |
| _version_ | 1811206359141580800 |
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| author | Alberto Ortiz-Vigón Sergio Martinez-Villa Iñaki Suarez Fabio Vignoletti Mariano Sanz |
| author_facet | Alberto Ortiz-Vigón Sergio Martinez-Villa Iñaki Suarez Fabio Vignoletti Mariano Sanz |
| author_sort | Alberto Ortiz-Vigón |
| collection | DOAJ |
| description | Abstract Background The osteoconductive properties of collagen containing xenogeneic bone blocks (CCXBB) remain unclear. The aim of this prospective single-arm clinical study was to assess the histological outcomes of CCXBB blocks used as bone replacement grafts for lateral bone augmentation procedures. Methods In 15 patients with severe horizontal alveolar ridge resorption, lateral augmentation procedures were performed using CCXBB as bone replacement grafts. Twenty-six weeks postoperatively, a re-entry procedure was performed to evaluate the bone width for adequate implant placement and two histological specimens were retrieved from each patient, one being processed for ground sectioning and the other for decalcified paraffin-included sections. In non-decalcified sections, the relative proportions occupied by bone, biomaterials, and connective tissue present in the biopsies were identified. In de-calcified sections, structures and cells positive for osteopontin (OPN), tartrate-resistant acid phosphatase activity (TRAP), osteocalcin (OSC), and alkaline phosphatase (ALP) were assessed. Results Soft tissue dehiscence occurred during the follow-up in 5 out of 15 patients (33.3%). The mean crest width at baseline was 2.78 mm (SD 0.57) and the mean crest width at re-entry was 6.90 mm (SD 1.22), with a mean ridge width increase of 4.12 mm (SD 1.32). Twenty-six bone biopsies were obtained from 13 patients. Histomorphometric analysis showed a mean of 26.90% (SD 12.21) of mineralized vital bone (MVB), 21.37% (SD 7.36) of residual CCXBB, 47.13% (SD 19.15) of non-mineralized tissue, and 0.92% of DBBM. The immunohistochemical analysis revealed a large number of OPN-positive cells 8.12% (SD 4.73), a lower proportion of TRAP positive multinuclear cells 5.09% (SD 4.91), OSC-positive cells 4.09% (SD 4.34), and a limited amount of ALP positive cells 1.63% (SD 2). Conclusions CCXBB achieved significant horizontal crestal width allowing for staged implant placement in most of the patients. In light of the histological outcomes and implant failures, special attention must be placed to prevent soft tissue dehiscence when CCXBB is used in severe atrophic alveolar crests. |
| first_indexed | 2024-04-12T03:46:07Z |
| format | Article |
| id | doaj.art-270096ec8aa442a69e2932e07b78b928 |
| institution | Directory Open Access Journal |
| issn | 2198-4034 |
| language | English |
| last_indexed | 2024-04-12T03:46:07Z |
| publishDate | 2017-06-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | International Journal of Implant Dentistry |
| spelling | doaj.art-270096ec8aa442a69e2932e07b78b9282022-12-22T03:49:07ZengSpringerOpenInternational Journal of Implant Dentistry2198-40342017-06-013111210.1186/s40729-017-0087-1Histomorphometric and immunohistochemical evaluation of collagen containing xenogeneic bone blocks used for lateral bone augmentation in staged implant placementAlberto Ortiz-Vigón0Sergio Martinez-Villa1Iñaki Suarez2Fabio Vignoletti3Mariano Sanz4ETEP Research Group, Facultad de Odontología, Universidad Complutense de MadridETEP Research Group, Facultad de Odontología, Universidad Complutense de MadridETEP Research Group, Facultad de Odontología, Universidad Complutense de MadridETEP Research Group, Facultad de Odontología, Universidad Complutense de MadridETEP Research Group, Facultad de Odontología, Universidad Complutense de MadridAbstract Background The osteoconductive properties of collagen containing xenogeneic bone blocks (CCXBB) remain unclear. The aim of this prospective single-arm clinical study was to assess the histological outcomes of CCXBB blocks used as bone replacement grafts for lateral bone augmentation procedures. Methods In 15 patients with severe horizontal alveolar ridge resorption, lateral augmentation procedures were performed using CCXBB as bone replacement grafts. Twenty-six weeks postoperatively, a re-entry procedure was performed to evaluate the bone width for adequate implant placement and two histological specimens were retrieved from each patient, one being processed for ground sectioning and the other for decalcified paraffin-included sections. In non-decalcified sections, the relative proportions occupied by bone, biomaterials, and connective tissue present in the biopsies were identified. In de-calcified sections, structures and cells positive for osteopontin (OPN), tartrate-resistant acid phosphatase activity (TRAP), osteocalcin (OSC), and alkaline phosphatase (ALP) were assessed. Results Soft tissue dehiscence occurred during the follow-up in 5 out of 15 patients (33.3%). The mean crest width at baseline was 2.78 mm (SD 0.57) and the mean crest width at re-entry was 6.90 mm (SD 1.22), with a mean ridge width increase of 4.12 mm (SD 1.32). Twenty-six bone biopsies were obtained from 13 patients. Histomorphometric analysis showed a mean of 26.90% (SD 12.21) of mineralized vital bone (MVB), 21.37% (SD 7.36) of residual CCXBB, 47.13% (SD 19.15) of non-mineralized tissue, and 0.92% of DBBM. The immunohistochemical analysis revealed a large number of OPN-positive cells 8.12% (SD 4.73), a lower proportion of TRAP positive multinuclear cells 5.09% (SD 4.91), OSC-positive cells 4.09% (SD 4.34), and a limited amount of ALP positive cells 1.63% (SD 2). Conclusions CCXBB achieved significant horizontal crestal width allowing for staged implant placement in most of the patients. In light of the histological outcomes and implant failures, special attention must be placed to prevent soft tissue dehiscence when CCXBB is used in severe atrophic alveolar crests.http://link.springer.com/article/10.1186/s40729-017-0087-1Clinical trialBone regenerationAlveolar ridge augmentationXenogeneic bone substitutesHeterograftsHistology |
| spellingShingle | Alberto Ortiz-Vigón Sergio Martinez-Villa Iñaki Suarez Fabio Vignoletti Mariano Sanz Histomorphometric and immunohistochemical evaluation of collagen containing xenogeneic bone blocks used for lateral bone augmentation in staged implant placement International Journal of Implant Dentistry Clinical trial Bone regeneration Alveolar ridge augmentation Xenogeneic bone substitutes Heterografts Histology |
| title | Histomorphometric and immunohistochemical evaluation of collagen containing xenogeneic bone blocks used for lateral bone augmentation in staged implant placement |
| title_full | Histomorphometric and immunohistochemical evaluation of collagen containing xenogeneic bone blocks used for lateral bone augmentation in staged implant placement |
| title_fullStr | Histomorphometric and immunohistochemical evaluation of collagen containing xenogeneic bone blocks used for lateral bone augmentation in staged implant placement |
| title_full_unstemmed | Histomorphometric and immunohistochemical evaluation of collagen containing xenogeneic bone blocks used for lateral bone augmentation in staged implant placement |
| title_short | Histomorphometric and immunohistochemical evaluation of collagen containing xenogeneic bone blocks used for lateral bone augmentation in staged implant placement |
| title_sort | histomorphometric and immunohistochemical evaluation of collagen containing xenogeneic bone blocks used for lateral bone augmentation in staged implant placement |
| topic | Clinical trial Bone regeneration Alveolar ridge augmentation Xenogeneic bone substitutes Heterografts Histology |
| url | http://link.springer.com/article/10.1186/s40729-017-0087-1 |
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