Generation of induced pluripotent stem cells (NIMHi004-A, NIMHi005-A and NIMHi006-A) from healthy individuals of Indian ethnicity with no mutation for Parkinson’s disease related genes
This study describes the generation and characterization of 3 induced pluripotent stem cell lines (iPSCs) generated by somatic reprogramming of peripheral blood mononuclear cells (PBMCs) obtained from healthy individuals. The reprogramming was carried out using non-integrating Sendai virus vectors e...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-04-01
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| Series: | Stem Cell Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506122000654 |
| _version_ | 1818995712156762112 |
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| author | Soham Jagtap Sowmithra Ravi Yadav Pramod Kumar Pal Indrani Datta |
| author_facet | Soham Jagtap Sowmithra Ravi Yadav Pramod Kumar Pal Indrani Datta |
| author_sort | Soham Jagtap |
| collection | DOAJ |
| description | This study describes the generation and characterization of 3 induced pluripotent stem cell lines (iPSCs) generated by somatic reprogramming of peripheral blood mononuclear cells (PBMCs) obtained from healthy individuals. The reprogramming was carried out using non-integrating Sendai virus vectors expressing hKOS, hc-myc and hKlf4. The donors did not carry any mutations for a panel of 13 genes associated with occurrence and progression of Parkinson’s disease (PD). These iPSC lines serve as age and gender matched control for the PD patient derived iPSC lines reported by us previously (Datta et al., 2020), neither did the samples have any chromosomal abnormalities. |
| first_indexed | 2024-12-20T21:18:12Z |
| format | Article |
| id | doaj.art-2707a91c486b454a839b6f29f2f18953 |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-12-20T21:18:12Z |
| publishDate | 2022-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-2707a91c486b454a839b6f29f2f189532022-12-21T19:26:22ZengElsevierStem Cell Research1873-50612022-04-0160102716Generation of induced pluripotent stem cells (NIMHi004-A, NIMHi005-A and NIMHi006-A) from healthy individuals of Indian ethnicity with no mutation for Parkinson’s disease related genesSoham Jagtap0 Sowmithra1Ravi Yadav2Pramod Kumar Pal3Indrani Datta4Department of Biophysics, National Institute of Mental Health and Neurosciences, Bengaluru 560029, Karnataka, IndiaDepartment of Biophysics, National Institute of Mental Health and Neurosciences, Bengaluru 560029, Karnataka, IndiaDepartment of Neurology, National Institute of Mental Health and Neurosciences, Bengaluru 560029, Karnataka, IndiaDepartment of Neurology, National Institute of Mental Health and Neurosciences, Bengaluru 560029, Karnataka, IndiaDepartment of Biophysics, National Institute of Mental Health and Neurosciences, Bengaluru 560029, Karnataka, India; Corresponding author at: Department of Biophysics, National Institute of Mental Health and Neurosciences, P.B. No – 2900, Hosur Road, Bengaluru 560029, Karnataka, India.This study describes the generation and characterization of 3 induced pluripotent stem cell lines (iPSCs) generated by somatic reprogramming of peripheral blood mononuclear cells (PBMCs) obtained from healthy individuals. The reprogramming was carried out using non-integrating Sendai virus vectors expressing hKOS, hc-myc and hKlf4. The donors did not carry any mutations for a panel of 13 genes associated with occurrence and progression of Parkinson’s disease (PD). These iPSC lines serve as age and gender matched control for the PD patient derived iPSC lines reported by us previously (Datta et al., 2020), neither did the samples have any chromosomal abnormalities.http://www.sciencedirect.com/science/article/pii/S1873506122000654Healthy control iPSCs for PD iPSCsScreened for PD-related genesIndian ethnicity |
| spellingShingle | Soham Jagtap Sowmithra Ravi Yadav Pramod Kumar Pal Indrani Datta Generation of induced pluripotent stem cells (NIMHi004-A, NIMHi005-A and NIMHi006-A) from healthy individuals of Indian ethnicity with no mutation for Parkinson’s disease related genes Stem Cell Research Healthy control iPSCs for PD iPSCs Screened for PD-related genes Indian ethnicity |
| title | Generation of induced pluripotent stem cells (NIMHi004-A, NIMHi005-A and NIMHi006-A) from healthy individuals of Indian ethnicity with no mutation for Parkinson’s disease related genes |
| title_full | Generation of induced pluripotent stem cells (NIMHi004-A, NIMHi005-A and NIMHi006-A) from healthy individuals of Indian ethnicity with no mutation for Parkinson’s disease related genes |
| title_fullStr | Generation of induced pluripotent stem cells (NIMHi004-A, NIMHi005-A and NIMHi006-A) from healthy individuals of Indian ethnicity with no mutation for Parkinson’s disease related genes |
| title_full_unstemmed | Generation of induced pluripotent stem cells (NIMHi004-A, NIMHi005-A and NIMHi006-A) from healthy individuals of Indian ethnicity with no mutation for Parkinson’s disease related genes |
| title_short | Generation of induced pluripotent stem cells (NIMHi004-A, NIMHi005-A and NIMHi006-A) from healthy individuals of Indian ethnicity with no mutation for Parkinson’s disease related genes |
| title_sort | generation of induced pluripotent stem cells nimhi004 a nimhi005 a and nimhi006 a from healthy individuals of indian ethnicity with no mutation for parkinson s disease related genes |
| topic | Healthy control iPSCs for PD iPSCs Screened for PD-related genes Indian ethnicity |
| url | http://www.sciencedirect.com/science/article/pii/S1873506122000654 |
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