Characterization of Cucumber Mosaic Virus Isolated from Passion Fruit (Passiflora edulis) in Korea

In July 2020, total RNA was extracted from passion fruit (Passiflora edulis) leaves showing virus symptoms such as chlorotic spots and vein banding in Haenam, South Korea. Cucumber mosaic virus (CMV)-HN2 was identified through reverse transcription polymerase chain reaction and sequencing analysis....

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Bibliographic Details
Main Authors: Ye-Yeong Kim, Tae-Seon Park, Ji-Soo Park, Dong-Joo Min, You-Seop Shin, Jin-Sung Hong
Format: Article
Language:English
Published: Hanrimwon Publishing Company 2024-03-01
Series:Research in Plant Disease
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Online Access:http://online-rpd.org/upload/pdf/RPD-2024-30-1-60.pdf
Description
Summary:In July 2020, total RNA was extracted from passion fruit (Passiflora edulis) leaves showing virus symptoms such as chlorotic spots and vein banding in Haenam, South Korea. Cucumber mosaic virus (CMV)-HN2 was identified through reverse transcription polymerase chain reaction and sequencing analysis. To confirm the biological characteristics of the CMV infecting passion fruit, 10 indicator plants were inoculated with CMV-HN2, and the results showed a typical CMV symptoms. Phylogenetic analysis based on the amino acid of the coat protein (CP) of CMVs revealed that the CMV passion fruit isolates belonged to subgroup I, among which CMV-HN2 belonged to subgroup IA. Additionally, CMVs isolated from passion fruit in Korea have amino acid sequence variation between the subgroup. Among them, CMV-HN2 had four to eight amino acid differences in CP from other CMV isolates from passion fruit. These results confirm the presence of genetic diversity in the CPs of passion fruit CMV isolates.
ISSN:1598-2262
2233-9191