Characterization of Cucumber Mosaic Virus Isolated from Passion Fruit (Passiflora edulis) in Korea
In July 2020, total RNA was extracted from passion fruit (Passiflora edulis) leaves showing virus symptoms such as chlorotic spots and vein banding in Haenam, South Korea. Cucumber mosaic virus (CMV)-HN2 was identified through reverse transcription polymerase chain reaction and sequencing analysis....
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Hanrimwon Publishing Company
2024-03-01
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| Series: | Research in Plant Disease |
| Subjects: | |
| Online Access: | http://online-rpd.org/upload/pdf/RPD-2024-30-1-60.pdf |
| _version_ | 1827283923185434624 |
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| author | Ye-Yeong Kim Tae-Seon Park Ji-Soo Park Dong-Joo Min You-Seop Shin Jin-Sung Hong |
| author_facet | Ye-Yeong Kim Tae-Seon Park Ji-Soo Park Dong-Joo Min You-Seop Shin Jin-Sung Hong |
| author_sort | Ye-Yeong Kim |
| collection | DOAJ |
| description | In July 2020, total RNA was extracted from passion fruit (Passiflora edulis) leaves showing virus symptoms such as chlorotic spots and vein banding in Haenam, South Korea. Cucumber mosaic virus (CMV)-HN2 was identified through reverse transcription polymerase chain reaction and sequencing analysis. To confirm the biological characteristics of the CMV infecting passion fruit, 10 indicator plants were inoculated with CMV-HN2, and the results showed a typical CMV symptoms. Phylogenetic analysis based on the amino acid of the coat protein (CP) of CMVs revealed that the CMV passion fruit isolates belonged to subgroup I, among which CMV-HN2 belonged to subgroup IA. Additionally, CMVs isolated from passion fruit in Korea have amino acid sequence variation between the subgroup. Among them, CMV-HN2 had four to eight amino acid differences in CP from other CMV isolates from passion fruit. These results confirm the presence of genetic diversity in the CPs of passion fruit CMV isolates. |
| first_indexed | 2024-04-24T09:45:12Z |
| format | Article |
| id | doaj.art-2708838069e44f4596ed30c45371bc19 |
| institution | Directory Open Access Journal |
| issn | 1598-2262 2233-9191 |
| language | English |
| last_indexed | 2024-04-24T09:45:12Z |
| publishDate | 2024-03-01 |
| publisher | Hanrimwon Publishing Company |
| record_format | Article |
| series | Research in Plant Disease |
| spelling | doaj.art-2708838069e44f4596ed30c45371bc192024-04-15T02:29:03ZengHanrimwon Publishing CompanyResearch in Plant Disease1598-22622233-91912024-03-01301606510.5423/RPD.2024.30.1.601831Characterization of Cucumber Mosaic Virus Isolated from Passion Fruit (Passiflora edulis) in KoreaYe-Yeong Kim0Tae-Seon Park1Ji-Soo Park2Dong-Joo Min3You-Seop Shin4Jin-Sung Hong5Department of Plant Medicine, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 24341, KoreaDepartment of Plant Medicine, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 24341, KoreaDepartment of Plant Medicine, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 24341, KoreaDepartment of Plant Medicine, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 24341, KoreaDepartment of Plant Medicine, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 24341, KoreaDepartment of Plant Medicine, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 24341, KoreaIn July 2020, total RNA was extracted from passion fruit (Passiflora edulis) leaves showing virus symptoms such as chlorotic spots and vein banding in Haenam, South Korea. Cucumber mosaic virus (CMV)-HN2 was identified through reverse transcription polymerase chain reaction and sequencing analysis. To confirm the biological characteristics of the CMV infecting passion fruit, 10 indicator plants were inoculated with CMV-HN2, and the results showed a typical CMV symptoms. Phylogenetic analysis based on the amino acid of the coat protein (CP) of CMVs revealed that the CMV passion fruit isolates belonged to subgroup I, among which CMV-HN2 belonged to subgroup IA. Additionally, CMVs isolated from passion fruit in Korea have amino acid sequence variation between the subgroup. Among them, CMV-HN2 had four to eight amino acid differences in CP from other CMV isolates from passion fruit. These results confirm the presence of genetic diversity in the CPs of passion fruit CMV isolates.http://online-rpd.org/upload/pdf/RPD-2024-30-1-60.pdfcoat protein genepassion fruitsubgroup ia |
| spellingShingle | Ye-Yeong Kim Tae-Seon Park Ji-Soo Park Dong-Joo Min You-Seop Shin Jin-Sung Hong Characterization of Cucumber Mosaic Virus Isolated from Passion Fruit (Passiflora edulis) in Korea Research in Plant Disease coat protein gene passion fruit subgroup ia |
| title | Characterization of Cucumber Mosaic Virus Isolated from Passion Fruit (Passiflora edulis) in Korea |
| title_full | Characterization of Cucumber Mosaic Virus Isolated from Passion Fruit (Passiflora edulis) in Korea |
| title_fullStr | Characterization of Cucumber Mosaic Virus Isolated from Passion Fruit (Passiflora edulis) in Korea |
| title_full_unstemmed | Characterization of Cucumber Mosaic Virus Isolated from Passion Fruit (Passiflora edulis) in Korea |
| title_short | Characterization of Cucumber Mosaic Virus Isolated from Passion Fruit (Passiflora edulis) in Korea |
| title_sort | characterization of cucumber mosaic virus isolated from passion fruit passiflora edulis in korea |
| topic | coat protein gene passion fruit subgroup ia |
| url | http://online-rpd.org/upload/pdf/RPD-2024-30-1-60.pdf |
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