RESPONSE SURFACE METHODOLOGY FOR THE OPTIMIZED PRODUCTION OF AN ALKALOPHILIC LACCASE FROM GAMMA-PROTEOBACTERIUM JB
Gamma-proteobacterium JB, an alkali-tolerant soil isolate, produced laccase (8X103 nkat/L) in M162 medium. The optimization of process conditions (pH, incubation time, agitation, and CuSO4 concentration) for laccase production during submerged fermentation was carried out using response surface meth...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
North Carolina State University
2009-05-01
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| Series: | BioResources |
| Subjects: | |
| Online Access: | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/viewFile/BioRes_04_2_0544_Singh_ASC_Response_Surface_Laccase/355 |
| Summary: | Gamma-proteobacterium JB, an alkali-tolerant soil isolate, produced laccase (8X103 nkat/L) in M162 medium. The optimization of process conditions (pH, incubation time, agitation, and CuSO4 concentration) for laccase production during submerged fermentation was carried out using response surface methodology (RSM) based on a central composite design (CCD). Maximum laccase production achieved was 7.4 X 104 nkat/L at pH 8.0, 210 rpm, 100 µM of CuSO4 after 60 h of incubation. This design of experiment methodology increased laccase production by 9.3 fold over the control. Experimental findings were in close agreement with the model predictions. |
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| ISSN: | 1930-2126 |