Exogenous Allantoin Confers Rapeseed (<i>Brassica campestris</i>) Tolerance to Simulated Drought by Improving Antioxidant Metabolism and Physiology
Allantoin is an emerging plant metabolite, but its role in conferring drought-induced oxidative stress is still elusive. Therefore, an experiment was devised to explore the role of allantoin (0.5 and 1.0 mM; foliar spray) in rapeseed (<i>Brassica campestris</i> cv. BARI Sarisha-17) under...
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| Format: | Article |
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MDPI AG
2023-07-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/12/8/1508 |
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| author | Md. Rakib Hossain Raihan Mira Rahman Anshu Rastogi Masayuki Fujita Mirza Hasanuzzaman |
| author_facet | Md. Rakib Hossain Raihan Mira Rahman Anshu Rastogi Masayuki Fujita Mirza Hasanuzzaman |
| author_sort | Md. Rakib Hossain Raihan |
| collection | DOAJ |
| description | Allantoin is an emerging plant metabolite, but its role in conferring drought-induced oxidative stress is still elusive. Therefore, an experiment was devised to explore the role of allantoin (0.5 and 1.0 mM; foliar spray) in rapeseed (<i>Brassica campestris</i> cv. BARI Sarisha-17) under drought. Seedlings at fifteen days of age were subjected to drought, maintaining soil moisture levels at 50% and 25% field capacities, while well-irrigated plants served as the control group. Drought-stressed plants exhibited increased levels of lipid peroxidation and hydrogen peroxide, electrolyte leakage, and impaired glyoxalase systems. Thus, the growth, biomass, and yield attributes of rapeseed were significantly impaired under drought. However, the allantoin-supplemented plants showed a notable increase in their contents of ascorbate and glutathione and decreased dehydroascorbate and glutathione disulfide contents under drought. Moreover, the activity of antioxidant enzymes such as ascorbate peroxidase, dehydroascorbate reductase, glutathione reductase, glutathione peroxidase, and catalase were accelerated with the allantoin spray and the glyoxalase system was also enhanced under drought. Moreover, the improvement in water balance with reduction in proline and potassium ion contents was also observed when allantoin was applied to the plants. Overall, the beneficial effects of allantoin supplementation resulted in the improved plant growth, biomass, and yield of rapeseed under drought conditions. These findings suggest that allantoin acts as an efficient metabolite in mitigating the oxidative stress caused by reactive oxygen species by enhancing antioxidant defense mechanisms and the glyoxalase system. |
| first_indexed | 2024-03-11T00:10:16Z |
| format | Article |
| id | doaj.art-2715121f160a4101b24043d0ab7d4e7e |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-11T00:10:16Z |
| publishDate | 2023-07-01 |
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| series | Antioxidants |
| spelling | doaj.art-2715121f160a4101b24043d0ab7d4e7e2023-11-19T00:00:53ZengMDPI AGAntioxidants2076-39212023-07-01128150810.3390/antiox12081508Exogenous Allantoin Confers Rapeseed (<i>Brassica campestris</i>) Tolerance to Simulated Drought by Improving Antioxidant Metabolism and PhysiologyMd. Rakib Hossain Raihan0Mira Rahman1Anshu Rastogi2Masayuki Fujita3Mirza Hasanuzzaman4Department of Agronomy, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka 1207, BangladeshDepartment of Agronomy, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka 1207, BangladeshLaboratory of Bioclimatology, Department of Ecology and Environmental Protection, Poznań University of Life Sciences, Piątkowska 94, 60-649 Poznan, PolandFaculty of Agriculture, Kagawa University, Kita-Gun, Kagawa, Miki-cho 761-0795, JapanDepartment of Agronomy, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka 1207, BangladeshAllantoin is an emerging plant metabolite, but its role in conferring drought-induced oxidative stress is still elusive. Therefore, an experiment was devised to explore the role of allantoin (0.5 and 1.0 mM; foliar spray) in rapeseed (<i>Brassica campestris</i> cv. BARI Sarisha-17) under drought. Seedlings at fifteen days of age were subjected to drought, maintaining soil moisture levels at 50% and 25% field capacities, while well-irrigated plants served as the control group. Drought-stressed plants exhibited increased levels of lipid peroxidation and hydrogen peroxide, electrolyte leakage, and impaired glyoxalase systems. Thus, the growth, biomass, and yield attributes of rapeseed were significantly impaired under drought. However, the allantoin-supplemented plants showed a notable increase in their contents of ascorbate and glutathione and decreased dehydroascorbate and glutathione disulfide contents under drought. Moreover, the activity of antioxidant enzymes such as ascorbate peroxidase, dehydroascorbate reductase, glutathione reductase, glutathione peroxidase, and catalase were accelerated with the allantoin spray and the glyoxalase system was also enhanced under drought. Moreover, the improvement in water balance with reduction in proline and potassium ion contents was also observed when allantoin was applied to the plants. Overall, the beneficial effects of allantoin supplementation resulted in the improved plant growth, biomass, and yield of rapeseed under drought conditions. These findings suggest that allantoin acts as an efficient metabolite in mitigating the oxidative stress caused by reactive oxygen species by enhancing antioxidant defense mechanisms and the glyoxalase system.https://www.mdpi.com/2076-3921/12/8/1508antioxidant defense systemascorbate–glutathione pathwayhydrogen peroxidestress elicitorwater stressureides |
| spellingShingle | Md. Rakib Hossain Raihan Mira Rahman Anshu Rastogi Masayuki Fujita Mirza Hasanuzzaman Exogenous Allantoin Confers Rapeseed (<i>Brassica campestris</i>) Tolerance to Simulated Drought by Improving Antioxidant Metabolism and Physiology Antioxidants antioxidant defense system ascorbate–glutathione pathway hydrogen peroxide stress elicitor water stress ureides |
| title | Exogenous Allantoin Confers Rapeseed (<i>Brassica campestris</i>) Tolerance to Simulated Drought by Improving Antioxidant Metabolism and Physiology |
| title_full | Exogenous Allantoin Confers Rapeseed (<i>Brassica campestris</i>) Tolerance to Simulated Drought by Improving Antioxidant Metabolism and Physiology |
| title_fullStr | Exogenous Allantoin Confers Rapeseed (<i>Brassica campestris</i>) Tolerance to Simulated Drought by Improving Antioxidant Metabolism and Physiology |
| title_full_unstemmed | Exogenous Allantoin Confers Rapeseed (<i>Brassica campestris</i>) Tolerance to Simulated Drought by Improving Antioxidant Metabolism and Physiology |
| title_short | Exogenous Allantoin Confers Rapeseed (<i>Brassica campestris</i>) Tolerance to Simulated Drought by Improving Antioxidant Metabolism and Physiology |
| title_sort | exogenous allantoin confers rapeseed i brassica campestris i tolerance to simulated drought by improving antioxidant metabolism and physiology |
| topic | antioxidant defense system ascorbate–glutathione pathway hydrogen peroxide stress elicitor water stress ureides |
| url | https://www.mdpi.com/2076-3921/12/8/1508 |
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