Reduced sputum expression of interferon-stimulated genes in severe COPD
Clarissa Hilzendeger,1 Jane da Silva,2 Monique Henket,1 Florence Schleich,1 Jean Louis Corhay,1 Tatiana Kebadze,3 Michael R Edwards,3 Patrick Mallia,3 Sebastian L Johnston,3 Renaud Louis1 1Department of Respiratory Medicine, Centre Hospitalier Universitaire (CHU) Liege, Groupe Interdisciplinaire de...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2016-06-01
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| Series: | International Journal of COPD |
| Subjects: | |
| Online Access: | https://www.dovepress.com/reduced-sputum-expression-of-interferon-stimulated-genes-in-severe-cop-peer-reviewed-article-COPD |
| _version_ | 1818482934453108736 |
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| author | Hilzendeger C da Silva J Henket M Schleich F Corhay JL Kebadze T Edwards MR Mallia P Johnston SL Louis R |
| author_facet | Hilzendeger C da Silva J Henket M Schleich F Corhay JL Kebadze T Edwards MR Mallia P Johnston SL Louis R |
| author_sort | Hilzendeger C |
| collection | DOAJ |
| description | Clarissa Hilzendeger,1 Jane da Silva,2 Monique Henket,1 Florence Schleich,1 Jean Louis Corhay,1 Tatiana Kebadze,3 Michael R Edwards,3 Patrick Mallia,3 Sebastian L Johnston,3 Renaud Louis1 1Department of Respiratory Medicine, Centre Hospitalier Universitaire (CHU) Liege, Groupe Interdisciplinaire de Génoprotéomique Appliquée (GIGA) I3 University of Liege, Belgium; 2Department of Medicine, Post-graduate Program in Health Sciences, University of Southern Santa Catarina, Palhoça-SC, Brazil; 3Airway Disease Division, Airway Disease Infection Section, National Heart and Lung Institute, Imperial College, London, UK Background: Exacerbations of COPD are frequent and commonly triggered by respiratory tract infections. The purpose of our study was to investigate innate immunity in stable COPD patients.Methods: Induced sputum was collected from 51 stable consecutive COPD patients recruited from the COPD Clinic of CHU Liege and 35 healthy subjects. Expression of interferons beta (IFN-β) and lambda1 (IL-29), IFN-stimulated genes (ISGs) MxA, OAS, and viperin were measured in total sputum cells by reverse transcription quantitative polymerase chain reaction (RT-qPCR). The presence of Picornaviruses was assessed by RT-PCR, while potential pathogenic microorganisms (PPM) were identified by sputum bacteriology.Results: Expression of IL-29 was found in 16 of 51 COPD patients (31%) and in nine of 35 healthy subjects (26%), while IFN-β was detected in six of 51 COPD patients (12%) and in two of 35 healthy subjects (6%). ISGs were easily detectable in both groups. In the whole group of COPD patients, OAS expression was decreased (P<0.05), while that of viperin was increased (P<0.01) compared to healthy subjects. No difference was found with respect to MxA. COPD patients from group D of Global Initiative for Chronic Obstructive Lung Disease (GOLD) had reduced expression of all three ISGs (P<0.01 for MxA, P<0.05 for OAS, and P<0.01 for viperin) as compared to those of group B patients. Picornaviruses were detected in eight of 51 (16%) COPD patients vs four of 33 (12%) healthy subjects, while PPM were detected in seven of 39 (18%) COPD patients and associated with raised sputum neutrophil counts. IFN-β expression was raised when either picornavirus or PPM were detected (P=0.06), but no difference was seen regarding IL-29 or ISGs.Conclusion: ISGs expression was reduced in severe COPD that may favor exacerbation and contribute to disease progress by altering response to infection. Keywords: COPD, innate immunity, interferon-β, interferon-λ1, interferon-stimulated genes |
| first_indexed | 2024-12-10T11:53:31Z |
| format | Article |
| id | doaj.art-271995afd2b44aca88fefeef99783d66 |
| institution | Directory Open Access Journal |
| issn | 1178-2005 |
| language | English |
| last_indexed | 2024-12-10T11:53:31Z |
| publishDate | 2016-06-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of COPD |
| spelling | doaj.art-271995afd2b44aca88fefeef99783d662022-12-22T01:49:51ZengDove Medical PressInternational Journal of COPD1178-20052016-06-012016Issue 11485149427703Reduced sputum expression of interferon-stimulated genes in severe COPDHilzendeger Cda Silva JHenket MSchleich FCorhay JLKebadze TEdwards MRMallia PJohnston SLLouis RClarissa Hilzendeger,1 Jane da Silva,2 Monique Henket,1 Florence Schleich,1 Jean Louis Corhay,1 Tatiana Kebadze,3 Michael R Edwards,3 Patrick Mallia,3 Sebastian L Johnston,3 Renaud Louis1 1Department of Respiratory Medicine, Centre Hospitalier Universitaire (CHU) Liege, Groupe Interdisciplinaire de Génoprotéomique Appliquée (GIGA) I3 University of Liege, Belgium; 2Department of Medicine, Post-graduate Program in Health Sciences, University of Southern Santa Catarina, Palhoça-SC, Brazil; 3Airway Disease Division, Airway Disease Infection Section, National Heart and Lung Institute, Imperial College, London, UK Background: Exacerbations of COPD are frequent and commonly triggered by respiratory tract infections. The purpose of our study was to investigate innate immunity in stable COPD patients.Methods: Induced sputum was collected from 51 stable consecutive COPD patients recruited from the COPD Clinic of CHU Liege and 35 healthy subjects. Expression of interferons beta (IFN-β) and lambda1 (IL-29), IFN-stimulated genes (ISGs) MxA, OAS, and viperin were measured in total sputum cells by reverse transcription quantitative polymerase chain reaction (RT-qPCR). The presence of Picornaviruses was assessed by RT-PCR, while potential pathogenic microorganisms (PPM) were identified by sputum bacteriology.Results: Expression of IL-29 was found in 16 of 51 COPD patients (31%) and in nine of 35 healthy subjects (26%), while IFN-β was detected in six of 51 COPD patients (12%) and in two of 35 healthy subjects (6%). ISGs were easily detectable in both groups. In the whole group of COPD patients, OAS expression was decreased (P<0.05), while that of viperin was increased (P<0.01) compared to healthy subjects. No difference was found with respect to MxA. COPD patients from group D of Global Initiative for Chronic Obstructive Lung Disease (GOLD) had reduced expression of all three ISGs (P<0.01 for MxA, P<0.05 for OAS, and P<0.01 for viperin) as compared to those of group B patients. Picornaviruses were detected in eight of 51 (16%) COPD patients vs four of 33 (12%) healthy subjects, while PPM were detected in seven of 39 (18%) COPD patients and associated with raised sputum neutrophil counts. IFN-β expression was raised when either picornavirus or PPM were detected (P=0.06), but no difference was seen regarding IL-29 or ISGs.Conclusion: ISGs expression was reduced in severe COPD that may favor exacerbation and contribute to disease progress by altering response to infection. Keywords: COPD, innate immunity, interferon-β, interferon-λ1, interferon-stimulated geneshttps://www.dovepress.com/reduced-sputum-expression-of-interferon-stimulated-genes-in-severe-cop-peer-reviewed-article-COPDCOPDInnate ImmunityInterferon-Interferon-1Interferon-stimulated genes. |
| spellingShingle | Hilzendeger C da Silva J Henket M Schleich F Corhay JL Kebadze T Edwards MR Mallia P Johnston SL Louis R Reduced sputum expression of interferon-stimulated genes in severe COPD International Journal of COPD COPD Innate Immunity Interferon- Interferon-1 Interferon-stimulated genes. |
| title | Reduced sputum expression of interferon-stimulated genes in severe COPD |
| title_full | Reduced sputum expression of interferon-stimulated genes in severe COPD |
| title_fullStr | Reduced sputum expression of interferon-stimulated genes in severe COPD |
| title_full_unstemmed | Reduced sputum expression of interferon-stimulated genes in severe COPD |
| title_short | Reduced sputum expression of interferon-stimulated genes in severe COPD |
| title_sort | reduced sputum expression of interferon stimulated genes in severe copd |
| topic | COPD Innate Immunity Interferon- Interferon-1 Interferon-stimulated genes. |
| url | https://www.dovepress.com/reduced-sputum-expression-of-interferon-stimulated-genes-in-severe-cop-peer-reviewed-article-COPD |
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