Identification of BST2 as a conjunctival epithelial stem/progenitor cell marker
Summary: The conjunctival epithelium consists of conjunctival epithelial cells and goblet cells derived from conjunctival epithelial stem/progenitor cells. However, the source of these cells is not well known because no specific markers for conjunctival epithelial stem/progenitor cells have been dis...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-07-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223010933 |
| _version_ | 1827895544751587328 |
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| author | Masahiro Kitao Ryuhei Hayashi Kimihito Nomi Reiko Kobayashi Tomohiko Katayama Hiroshi Takayanagi Akiko Oguchi Yasuhiro Murakawa Kohji Nishida |
| author_facet | Masahiro Kitao Ryuhei Hayashi Kimihito Nomi Reiko Kobayashi Tomohiko Katayama Hiroshi Takayanagi Akiko Oguchi Yasuhiro Murakawa Kohji Nishida |
| author_sort | Masahiro Kitao |
| collection | DOAJ |
| description | Summary: The conjunctival epithelium consists of conjunctival epithelial cells and goblet cells derived from conjunctival epithelial stem/progenitor cells. However, the source of these cells is not well known because no specific markers for conjunctival epithelial stem/progenitor cells have been discovered. Therefore, to identify conjunctival epithelial stem/progenitor cell markers, we performed single-cell RNA sequencing of a conjunctival epithelial cell population derived from human-induced pluripotent stem cells (hiPSCs). The following conjunctival epithelial markers were identified: BST2, SLC2A3, AGR2, TMEM54, OLR1, and TRIM29. Notably, BST2 was strongly positive in the basal conjunctival epithelium, which is thought to be rich in stem/progenitor cells. Moreover, BST2 was able to sort conjunctival epithelial stem/progenitor cells from hiPSC-derived ocular surface epithelial cell populations. BST2-positive cells were highly proliferative and capable of successfully generating conjunctival epithelial sheets containing goblet cells. In conclusion, BST2 has been identified as a specific marker of conjunctival epithelial stem/progenitor cells. |
| first_indexed | 2024-03-12T22:22:29Z |
| format | Article |
| id | doaj.art-271c33d1775d4633846db8931c9df2d4 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-03-12T22:22:29Z |
| publishDate | 2023-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-271c33d1775d4633846db8931c9df2d42023-07-23T04:55:09ZengElsevieriScience2589-00422023-07-01267107016Identification of BST2 as a conjunctival epithelial stem/progenitor cell markerMasahiro Kitao0Ryuhei Hayashi1Kimihito Nomi2Reiko Kobayashi3Tomohiko Katayama4Hiroshi Takayanagi5Akiko Oguchi6Yasuhiro Murakawa7Kohji Nishida8Department of Ophthalmology, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, JapanDepartment of Ophthalmology, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan; Department of Stem Cells and Applied Medicine, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan; Integrated Frontier Research for Medical Science Division, Institute for Open and Transdisciplinary Research Initiatives, Osaka University, Suita, Osaka 565-0871, Japan; Corresponding authorBusiness Strategy Office, ROHTO Pharmaceutical, Osaka, Osaka 544-0012, JapanDepartment of Ophthalmology, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, JapanDepartment of Ophthalmology, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, JapanDepartment of Ophthalmology, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan; Department of Stem Cells and Applied Medicine, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, JapanRIKEN-IFOM Joint Laboratory for Cancer Genomics, RIKEN Center for Integrative Medical Sciences, Yokohama, Kanagawa 230-0045, JapanRIKEN-IFOM Joint Laboratory for Cancer Genomics, RIKEN Center for Integrative Medical Sciences, Yokohama, Kanagawa 230-0045, Japan; Institute for the Advanced Study of Human Biology (ASHBi), Kyoto University, Kyoto, Kyoto 606-8501, JapanDepartment of Ophthalmology, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan; Integrated Frontier Research for Medical Science Division, Institute for Open and Transdisciplinary Research Initiatives, Osaka University, Suita, Osaka 565-0871, Japan; Premium Research Institute for Human Metaverse Medicine (WPI-PRIMe), Osaka University, Suita, Osaka 565-0871, Japan; Corresponding authorSummary: The conjunctival epithelium consists of conjunctival epithelial cells and goblet cells derived from conjunctival epithelial stem/progenitor cells. However, the source of these cells is not well known because no specific markers for conjunctival epithelial stem/progenitor cells have been discovered. Therefore, to identify conjunctival epithelial stem/progenitor cell markers, we performed single-cell RNA sequencing of a conjunctival epithelial cell population derived from human-induced pluripotent stem cells (hiPSCs). The following conjunctival epithelial markers were identified: BST2, SLC2A3, AGR2, TMEM54, OLR1, and TRIM29. Notably, BST2 was strongly positive in the basal conjunctival epithelium, which is thought to be rich in stem/progenitor cells. Moreover, BST2 was able to sort conjunctival epithelial stem/progenitor cells from hiPSC-derived ocular surface epithelial cell populations. BST2-positive cells were highly proliferative and capable of successfully generating conjunctival epithelial sheets containing goblet cells. In conclusion, BST2 has been identified as a specific marker of conjunctival epithelial stem/progenitor cells.http://www.sciencedirect.com/science/article/pii/S2589004223010933Cell biologyStem cells researchTranscriptomics |
| spellingShingle | Masahiro Kitao Ryuhei Hayashi Kimihito Nomi Reiko Kobayashi Tomohiko Katayama Hiroshi Takayanagi Akiko Oguchi Yasuhiro Murakawa Kohji Nishida Identification of BST2 as a conjunctival epithelial stem/progenitor cell marker iScience Cell biology Stem cells research Transcriptomics |
| title | Identification of BST2 as a conjunctival epithelial stem/progenitor cell marker |
| title_full | Identification of BST2 as a conjunctival epithelial stem/progenitor cell marker |
| title_fullStr | Identification of BST2 as a conjunctival epithelial stem/progenitor cell marker |
| title_full_unstemmed | Identification of BST2 as a conjunctival epithelial stem/progenitor cell marker |
| title_short | Identification of BST2 as a conjunctival epithelial stem/progenitor cell marker |
| title_sort | identification of bst2 as a conjunctival epithelial stem progenitor cell marker |
| topic | Cell biology Stem cells research Transcriptomics |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223010933 |
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