Polymorphism rs652438 of gene <i>mmp12</i> and oxidative DNA damage in bronchial asthma: An experimental non-randomised study
Background. Personalised medicine is an avenue to create technologies for individual prognosis of the disease onset and development. The identification of individual gene haplotypes is prerequisite to detecting predispositions to multifactorial diseases. The level of serum 8-oxoguanine is an indicat...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
Ministry of Healthcare of the Russian Federation. “Kuban State Medical University”
2022-06-01
|
| Series: | Кубанский научный медицинский вестник |
| Subjects: | |
| Online Access: | https://ksma.elpub.ru/jour/article/view/2861 |
| _version_ | 1797295328998719488 |
|---|---|
| author | I. I. Pavlyuchenko L. R. Gusaruk E. E. Tekutskaya Yu. I. Prozorovskaya E. A. Pocheshkhova |
| author_facet | I. I. Pavlyuchenko L. R. Gusaruk E. E. Tekutskaya Yu. I. Prozorovskaya E. A. Pocheshkhova |
| author_sort | I. I. Pavlyuchenko |
| collection | DOAJ |
| description | Background. Personalised medicine is an avenue to create technologies for individual prognosis of the disease onset and development. The identification of individual gene haplotypes is prerequisite to detecting predispositions to multifactorial diseases. The level of serum 8-oxoguanine is an indicator of genotoxic stress underlying many pathologies.Objectives. A study of associations of mmp12 gene’s polymorphic variant rs652438 and the nature of genome oxidative damage in bronchial asthma.Methods. Genotyping of polymorphic variant rs652438 of gene mmp12 was performed using TaqMan-probe real-time PCR assays. The gene variant association with disease was assessed by odds ratio. The degree of DNA oxidative damage was estimated by 8-oxoguanine serum concentrations determined in monoclonal antibody-based enzyme immunoassays. The StatPro software package with StatTools (Palisade Corporation, USA) was used for statistical data processing.Results. The haplotype and allele frequencies were established for polymorphic locus rs652438 of the mmp12 gene in the control and bronchial asthma cohorts. Heterozygotes were shown to differ significantly; the estimate was 2.3-fold higher in the control vs. bronchial asthma (BA) cohort (p < 0.05). The AA and GG haplotype frequencies did not differ significantly. The minor allele G odds ratio (OR = 0.362, CI 95% 0.134–0.975) suggests its protective effect. This may be associated with a lowering activity of the encoded macrophage metalloelastase enzyme, which results in a poorer extracellular matrix destruction in the bronchial tree. The baseline 8-oxoG levels in the control and BA samples were 6.4 and 9.4 ng/mL, respectively (U = 25, Ucut-off = 23; p >0.05). An in vitro electromagnetic exposure of varying frequency leads to a significant oxidative genomic damage in both cohorts and an earlier reparative depletion in bronchial asthma vs. control.Conclusion. A protective effect of minor allele G against pathology has been demonstrated. Adaptations to oxidative genomic stress in bronchial asthma manifest by an impaired resistance to in vitro high-intensity electromagnetic exposures. |
| first_indexed | 2024-03-07T21:47:13Z |
| format | Article |
| id | doaj.art-271ea9c39f8e469dbbd0629c315069f8 |
| institution | Directory Open Access Journal |
| issn | 1608-6228 2541-9544 |
| language | Russian |
| last_indexed | 2024-03-07T21:47:13Z |
| publishDate | 2022-06-01 |
| publisher | Ministry of Healthcare of the Russian Federation. “Kuban State Medical University” |
| record_format | Article |
| series | Кубанский научный медицинский вестник |
| spelling | doaj.art-271ea9c39f8e469dbbd0629c315069f82024-02-25T10:57:26ZrusMinistry of Healthcare of the Russian Federation. “Kuban State Medical University”Кубанский научный медицинский вестник1608-62282541-95442022-06-01293627510.25207/1608-6228-2022-29-3-62-751316Polymorphism rs652438 of gene <i>mmp12</i> and oxidative DNA damage in bronchial asthma: An experimental non-randomised studyI. I. Pavlyuchenko0L. R. Gusaruk1E. E. Tekutskaya2Yu. I. Prozorovskaya3E. A. Pocheshkhova4Kuban State Medical UniversityKuban State Medical UniversityKuban State UniversityKuban State Medical UniversityKuban State Medical UniversityBackground. Personalised medicine is an avenue to create technologies for individual prognosis of the disease onset and development. The identification of individual gene haplotypes is prerequisite to detecting predispositions to multifactorial diseases. The level of serum 8-oxoguanine is an indicator of genotoxic stress underlying many pathologies.Objectives. A study of associations of mmp12 gene’s polymorphic variant rs652438 and the nature of genome oxidative damage in bronchial asthma.Methods. Genotyping of polymorphic variant rs652438 of gene mmp12 was performed using TaqMan-probe real-time PCR assays. The gene variant association with disease was assessed by odds ratio. The degree of DNA oxidative damage was estimated by 8-oxoguanine serum concentrations determined in monoclonal antibody-based enzyme immunoassays. The StatPro software package with StatTools (Palisade Corporation, USA) was used for statistical data processing.Results. The haplotype and allele frequencies were established for polymorphic locus rs652438 of the mmp12 gene in the control and bronchial asthma cohorts. Heterozygotes were shown to differ significantly; the estimate was 2.3-fold higher in the control vs. bronchial asthma (BA) cohort (p < 0.05). The AA and GG haplotype frequencies did not differ significantly. The minor allele G odds ratio (OR = 0.362, CI 95% 0.134–0.975) suggests its protective effect. This may be associated with a lowering activity of the encoded macrophage metalloelastase enzyme, which results in a poorer extracellular matrix destruction in the bronchial tree. The baseline 8-oxoG levels in the control and BA samples were 6.4 and 9.4 ng/mL, respectively (U = 25, Ucut-off = 23; p >0.05). An in vitro electromagnetic exposure of varying frequency leads to a significant oxidative genomic damage in both cohorts and an earlier reparative depletion in bronchial asthma vs. control.Conclusion. A protective effect of minor allele G against pathology has been demonstrated. Adaptations to oxidative genomic stress in bronchial asthma manifest by an impaired resistance to in vitro high-intensity electromagnetic exposures.https://ksma.elpub.ru/jour/article/view/2861bronchial asthmamatrix metalloproteinase genesoxidative dna damage8-oxoguanine |
| spellingShingle | I. I. Pavlyuchenko L. R. Gusaruk E. E. Tekutskaya Yu. I. Prozorovskaya E. A. Pocheshkhova Polymorphism rs652438 of gene <i>mmp12</i> and oxidative DNA damage in bronchial asthma: An experimental non-randomised study Кубанский научный медицинский вестник bronchial asthma matrix metalloproteinase genes oxidative dna damage 8-oxoguanine |
| title | Polymorphism rs652438 of gene <i>mmp12</i> and oxidative DNA damage in bronchial asthma: An experimental non-randomised study |
| title_full | Polymorphism rs652438 of gene <i>mmp12</i> and oxidative DNA damage in bronchial asthma: An experimental non-randomised study |
| title_fullStr | Polymorphism rs652438 of gene <i>mmp12</i> and oxidative DNA damage in bronchial asthma: An experimental non-randomised study |
| title_full_unstemmed | Polymorphism rs652438 of gene <i>mmp12</i> and oxidative DNA damage in bronchial asthma: An experimental non-randomised study |
| title_short | Polymorphism rs652438 of gene <i>mmp12</i> and oxidative DNA damage in bronchial asthma: An experimental non-randomised study |
| title_sort | polymorphism rs652438 of gene i mmp12 i and oxidative dna damage in bronchial asthma an experimental non randomised study |
| topic | bronchial asthma matrix metalloproteinase genes oxidative dna damage 8-oxoguanine |
| url | https://ksma.elpub.ru/jour/article/view/2861 |
| work_keys_str_mv | AT iipavlyuchenko polymorphismrs652438ofgeneimmp12iandoxidativednadamageinbronchialasthmaanexperimentalnonrandomisedstudy AT lrgusaruk polymorphismrs652438ofgeneimmp12iandoxidativednadamageinbronchialasthmaanexperimentalnonrandomisedstudy AT eetekutskaya polymorphismrs652438ofgeneimmp12iandoxidativednadamageinbronchialasthmaanexperimentalnonrandomisedstudy AT yuiprozorovskaya polymorphismrs652438ofgeneimmp12iandoxidativednadamageinbronchialasthmaanexperimentalnonrandomisedstudy AT eapocheshkhova polymorphismrs652438ofgeneimmp12iandoxidativednadamageinbronchialasthmaanexperimentalnonrandomisedstudy |