Octanoic Acid-Enrichment Diet Improves Endurance Capacity and Reprograms Mitochondrial Biogenesis in Skeletal Muscle of Mice
Background: Medium Chain Fatty Acids (MCFAs) are a dietary supplement that exhibit interesting properties, due to their smaller molecular size. The acute consumption of MCFAs is expected to enhance exercise performance. However, the short-term effects of MCFAs on endurance performance remains poorly...
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MDPI AG
2022-06-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/14/13/2721 |
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| author | Anouk Charlot Lucas Morel Anthony Bringolf Isabelle Georg Anne-Laure Charles Fabienne Goupilleau Bernard Geny Joffrey Zoll |
| author_facet | Anouk Charlot Lucas Morel Anthony Bringolf Isabelle Georg Anne-Laure Charles Fabienne Goupilleau Bernard Geny Joffrey Zoll |
| author_sort | Anouk Charlot |
| collection | DOAJ |
| description | Background: Medium Chain Fatty Acids (MCFAs) are a dietary supplement that exhibit interesting properties, due to their smaller molecular size. The acute consumption of MCFAs is expected to enhance exercise performance. However, the short-term effects of MCFAs on endurance performance remains poorly understood. The aim of our study is to evaluate the octanoic acid (C8)-rich diet effect on endurance capacity, and to explore their molecular and cellular effects. Methods: C57BL/6J mice were fed with a chow diet (Control group) or an octanoic acid-rich diet (C8 diet) for 6 weeks. Spontaneous activity, submaximal and maximal exercise tests were carried out to characterize the exercise capacities of the mice. Beta-oxidation and mitochondrial biogenesis pathways were explored in skeletal muscle by RT-qPCR, Western Blot (Quadriceps) and histochemical staining (Gastrocnemius). Results: Mice fed with a C8-rich diet presented a higher spontaneous activity (<i>p <</i> 0.05) and endurance capacities (<i>p <</i> 0.05) than the control, but no effect on maximal effort was observed. They also presented changes in the skeletal muscle metabolic phenotype, with a higher number of the oxidative fibers, rich in mitochondria. At the molecular level, the C8-diet induced an AMPK activation (<i>p <</i> 0.05), associated with a significant increase in PGC1a and CS gene expression and protein levels. Conclusion: Our study provided evidence that C8-enrichment as a food supplementation improves endurance capacities and activates mitochondrial biogenesis pathways leading to higher skeletal muscle oxidative capacities. |
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| last_indexed | 2024-03-09T03:58:14Z |
| publishDate | 2022-06-01 |
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| series | Nutrients |
| spelling | doaj.art-27200589d92c4159a88254a0cbaf40ee2023-12-03T14:16:48ZengMDPI AGNutrients2072-66432022-06-011413272110.3390/nu14132721Octanoic Acid-Enrichment Diet Improves Endurance Capacity and Reprograms Mitochondrial Biogenesis in Skeletal Muscle of MiceAnouk Charlot0Lucas Morel1Anthony Bringolf2Isabelle Georg3Anne-Laure Charles4Fabienne Goupilleau5Bernard Geny6Joffrey Zoll7Centre de Recherche de Biomédecine de Strasbourg, UR 3072 Mitochondrie, Stress Oxydant et Protection Musculaire, Université de Strasbourg, 67000 Strasbourg, FranceDepartment of Infection and Immunity, Luxembourg Institute of Health, 4354 Esch-sur-Alzette, LuxembourgCentre de Recherche de Biomédecine de Strasbourg, UR 3072 Mitochondrie, Stress Oxydant et Protection Musculaire, Université de Strasbourg, 67000 Strasbourg, FranceCentre de Recherche de Biomédecine de Strasbourg, UR 3072 Mitochondrie, Stress Oxydant et Protection Musculaire, Université de Strasbourg, 67000 Strasbourg, FranceCentre de Recherche de Biomédecine de Strasbourg, UR 3072 Mitochondrie, Stress Oxydant et Protection Musculaire, Université de Strasbourg, 67000 Strasbourg, FranceCentre de Recherche de Biomédecine de Strasbourg, UR 3072 Mitochondrie, Stress Oxydant et Protection Musculaire, Université de Strasbourg, 67000 Strasbourg, FranceCentre de Recherche de Biomédecine de Strasbourg, UR 3072 Mitochondrie, Stress Oxydant et Protection Musculaire, Université de Strasbourg, 67000 Strasbourg, FranceCentre de Recherche de Biomédecine de Strasbourg, UR 3072 Mitochondrie, Stress Oxydant et Protection Musculaire, Université de Strasbourg, 67000 Strasbourg, FranceBackground: Medium Chain Fatty Acids (MCFAs) are a dietary supplement that exhibit interesting properties, due to their smaller molecular size. The acute consumption of MCFAs is expected to enhance exercise performance. However, the short-term effects of MCFAs on endurance performance remains poorly understood. The aim of our study is to evaluate the octanoic acid (C8)-rich diet effect on endurance capacity, and to explore their molecular and cellular effects. Methods: C57BL/6J mice were fed with a chow diet (Control group) or an octanoic acid-rich diet (C8 diet) for 6 weeks. Spontaneous activity, submaximal and maximal exercise tests were carried out to characterize the exercise capacities of the mice. Beta-oxidation and mitochondrial biogenesis pathways were explored in skeletal muscle by RT-qPCR, Western Blot (Quadriceps) and histochemical staining (Gastrocnemius). Results: Mice fed with a C8-rich diet presented a higher spontaneous activity (<i>p <</i> 0.05) and endurance capacities (<i>p <</i> 0.05) than the control, but no effect on maximal effort was observed. They also presented changes in the skeletal muscle metabolic phenotype, with a higher number of the oxidative fibers, rich in mitochondria. At the molecular level, the C8-diet induced an AMPK activation (<i>p <</i> 0.05), associated with a significant increase in PGC1a and CS gene expression and protein levels. Conclusion: Our study provided evidence that C8-enrichment as a food supplementation improves endurance capacities and activates mitochondrial biogenesis pathways leading to higher skeletal muscle oxidative capacities.https://www.mdpi.com/2072-6643/14/13/2721medium chain fatty acidoctanoic acidendurancemitochondrial biogenesisskeletal muscle |
| spellingShingle | Anouk Charlot Lucas Morel Anthony Bringolf Isabelle Georg Anne-Laure Charles Fabienne Goupilleau Bernard Geny Joffrey Zoll Octanoic Acid-Enrichment Diet Improves Endurance Capacity and Reprograms Mitochondrial Biogenesis in Skeletal Muscle of Mice Nutrients medium chain fatty acid octanoic acid endurance mitochondrial biogenesis skeletal muscle |
| title | Octanoic Acid-Enrichment Diet Improves Endurance Capacity and Reprograms Mitochondrial Biogenesis in Skeletal Muscle of Mice |
| title_full | Octanoic Acid-Enrichment Diet Improves Endurance Capacity and Reprograms Mitochondrial Biogenesis in Skeletal Muscle of Mice |
| title_fullStr | Octanoic Acid-Enrichment Diet Improves Endurance Capacity and Reprograms Mitochondrial Biogenesis in Skeletal Muscle of Mice |
| title_full_unstemmed | Octanoic Acid-Enrichment Diet Improves Endurance Capacity and Reprograms Mitochondrial Biogenesis in Skeletal Muscle of Mice |
| title_short | Octanoic Acid-Enrichment Diet Improves Endurance Capacity and Reprograms Mitochondrial Biogenesis in Skeletal Muscle of Mice |
| title_sort | octanoic acid enrichment diet improves endurance capacity and reprograms mitochondrial biogenesis in skeletal muscle of mice |
| topic | medium chain fatty acid octanoic acid endurance mitochondrial biogenesis skeletal muscle |
| url | https://www.mdpi.com/2072-6643/14/13/2721 |
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