Three-dimensional imaging of the mouse neurovasculature with magnetic resonance microscopy.
Knowledge of the three-dimensional (3D) architecture of blood vessels in the brain is crucial because the progression of various neuropathologies ranging from Alzheimer's disease to brain tumors involves anomalous blood vessels. The challenges in obtaining such data from patients, in conjunctio...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3144917?pdf=render |
| _version_ | 1818020065693401088 |
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| author | Arvind P Pathak Eugene Kim Jiangyang Zhang Melina V Jones |
| author_facet | Arvind P Pathak Eugene Kim Jiangyang Zhang Melina V Jones |
| author_sort | Arvind P Pathak |
| collection | DOAJ |
| description | Knowledge of the three-dimensional (3D) architecture of blood vessels in the brain is crucial because the progression of various neuropathologies ranging from Alzheimer's disease to brain tumors involves anomalous blood vessels. The challenges in obtaining such data from patients, in conjunction with development of mouse models of neuropathology, have made the murine brain indispensable for investigating disease induced neurovascular changes. Here we describe a novel method for "whole brain" 3D mapping of murine neurovasculature using magnetic resonance microscopy (μMRI). This approach preserves the vascular and white matter tract architecture, and can be combined with complementary MRI contrast mechanisms such as diffusion tensor imaging (DTI) to examine the interplay between the vasculature and white matter reorganization that often characterizes neuropathologies. Following validation with micro computed tomography (μCT) and optical microscopy, we demonstrate the utility of this method by: (i) combined 3D imaging of angiogenesis and white matter reorganization in both, invasive and non-invasive brain tumor models; (ii) characterizing the morphological heterogeneity of the vascular phenotype in the murine brain; and (iii) conducting "multi-scale" imaging of brain tumor angiogenesis, wherein we directly compared in vivo MRI blood volume measurements with ex vivo vasculature data. |
| first_indexed | 2024-04-14T08:01:07Z |
| format | Article |
| id | doaj.art-27202f482d7b4a448c7cff92fc9f4255 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-14T08:01:07Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-27202f482d7b4a448c7cff92fc9f42552022-12-22T02:04:54ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0167e2264310.1371/journal.pone.0022643Three-dimensional imaging of the mouse neurovasculature with magnetic resonance microscopy.Arvind P PathakEugene KimJiangyang ZhangMelina V JonesKnowledge of the three-dimensional (3D) architecture of blood vessels in the brain is crucial because the progression of various neuropathologies ranging from Alzheimer's disease to brain tumors involves anomalous blood vessels. The challenges in obtaining such data from patients, in conjunction with development of mouse models of neuropathology, have made the murine brain indispensable for investigating disease induced neurovascular changes. Here we describe a novel method for "whole brain" 3D mapping of murine neurovasculature using magnetic resonance microscopy (μMRI). This approach preserves the vascular and white matter tract architecture, and can be combined with complementary MRI contrast mechanisms such as diffusion tensor imaging (DTI) to examine the interplay between the vasculature and white matter reorganization that often characterizes neuropathologies. Following validation with micro computed tomography (μCT) and optical microscopy, we demonstrate the utility of this method by: (i) combined 3D imaging of angiogenesis and white matter reorganization in both, invasive and non-invasive brain tumor models; (ii) characterizing the morphological heterogeneity of the vascular phenotype in the murine brain; and (iii) conducting "multi-scale" imaging of brain tumor angiogenesis, wherein we directly compared in vivo MRI blood volume measurements with ex vivo vasculature data.http://europepmc.org/articles/PMC3144917?pdf=render |
| spellingShingle | Arvind P Pathak Eugene Kim Jiangyang Zhang Melina V Jones Three-dimensional imaging of the mouse neurovasculature with magnetic resonance microscopy. PLoS ONE |
| title | Three-dimensional imaging of the mouse neurovasculature with magnetic resonance microscopy. |
| title_full | Three-dimensional imaging of the mouse neurovasculature with magnetic resonance microscopy. |
| title_fullStr | Three-dimensional imaging of the mouse neurovasculature with magnetic resonance microscopy. |
| title_full_unstemmed | Three-dimensional imaging of the mouse neurovasculature with magnetic resonance microscopy. |
| title_short | Three-dimensional imaging of the mouse neurovasculature with magnetic resonance microscopy. |
| title_sort | three dimensional imaging of the mouse neurovasculature with magnetic resonance microscopy |
| url | http://europepmc.org/articles/PMC3144917?pdf=render |
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