Optimization of <i>Bacillus amyloliquefaciens</i> BLB369 Culture Medium by Response Surface Methodology for Low Cost Production of Antifungal Activity
<i>Bacillus amyloliquefaciens</i> BLB369 is an important plant growth-promoting bacterium, which produces antifungal compounds. A statistics-based experimental design was used to optimize a liquid culture medium using inexpensive substrates for increasing its antifungal activity. A Plack...
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| Format: | Article |
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MDPI AG
2022-04-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/4/830 |
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| author | Imen Zalila-Kolsi Sameh Kessentini Slim Tounsi Kaïs Jamoussi |
| author_facet | Imen Zalila-Kolsi Sameh Kessentini Slim Tounsi Kaïs Jamoussi |
| author_sort | Imen Zalila-Kolsi |
| collection | DOAJ |
| description | <i>Bacillus amyloliquefaciens</i> BLB369 is an important plant growth-promoting bacterium, which produces antifungal compounds. A statistics-based experimental design was used to optimize a liquid culture medium using inexpensive substrates for increasing its antifungal activity. A Plackett–Burman design was first applied to elucidate medium components having significant effects on antifungal production. Then the steepest ascent method was employed to approach the experimental design space, followed by an application of central composite design. Three factors were retained (candy waste, peptone, and sodium chloride), and polynomial and original trigonometric models fitted the antifungal activity. The trigonometric model ensured a better fit. The contour and surface plots showed concentric increasing levels pointing out an optimized activity. Hence, the polynomial and trigonometric models showed a maximal antifungal activity of 251.9 (AU/mL) and 255.5 (AU/mL) for (19.17, 19.88, 3.75) (g/L) and (19.61, 20, 3.7) (g/L) of candy waste, peptone, and NaCl, respectively. This study provides a potential strategy for improving the fermentation of <i>B. amyloliquefaciens</i> BLB369 in low-cost media for large-scale industrial production. |
| first_indexed | 2024-03-09T04:22:41Z |
| format | Article |
| id | doaj.art-2726342a4dc049f99c5be8a8847cb68a |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T04:22:41Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-2726342a4dc049f99c5be8a8847cb68a2023-12-03T13:45:20ZengMDPI AGMicroorganisms2076-26072022-04-0110483010.3390/microorganisms10040830Optimization of <i>Bacillus amyloliquefaciens</i> BLB369 Culture Medium by Response Surface Methodology for Low Cost Production of Antifungal ActivityImen Zalila-Kolsi0Sameh Kessentini1Slim Tounsi2Kaïs Jamoussi3Laboratory of Biopesticides, Centre of Biotechnology of Sfax, University of Sfax, P.O. Box 1177, Sfax 3018, TunisiaLaboratory of Probability and Statistics, Faculty of Sciences of Sfax, University of Sfax, P.O. Box 1171, Sfax 3000, TunisiaLaboratory of Biopesticides, Centre of Biotechnology of Sfax, University of Sfax, P.O. Box 1177, Sfax 3018, TunisiaLaboratory of Biopesticides, Centre of Biotechnology of Sfax, University of Sfax, P.O. Box 1177, Sfax 3018, Tunisia<i>Bacillus amyloliquefaciens</i> BLB369 is an important plant growth-promoting bacterium, which produces antifungal compounds. A statistics-based experimental design was used to optimize a liquid culture medium using inexpensive substrates for increasing its antifungal activity. A Plackett–Burman design was first applied to elucidate medium components having significant effects on antifungal production. Then the steepest ascent method was employed to approach the experimental design space, followed by an application of central composite design. Three factors were retained (candy waste, peptone, and sodium chloride), and polynomial and original trigonometric models fitted the antifungal activity. The trigonometric model ensured a better fit. The contour and surface plots showed concentric increasing levels pointing out an optimized activity. Hence, the polynomial and trigonometric models showed a maximal antifungal activity of 251.9 (AU/mL) and 255.5 (AU/mL) for (19.17, 19.88, 3.75) (g/L) and (19.61, 20, 3.7) (g/L) of candy waste, peptone, and NaCl, respectively. This study provides a potential strategy for improving the fermentation of <i>B. amyloliquefaciens</i> BLB369 in low-cost media for large-scale industrial production.https://www.mdpi.com/2076-2607/10/4/830antifungal activityresponse surface methodologyPlackett–Burman designcentral composite designpolynomial and trigonometric regression models |
| spellingShingle | Imen Zalila-Kolsi Sameh Kessentini Slim Tounsi Kaïs Jamoussi Optimization of <i>Bacillus amyloliquefaciens</i> BLB369 Culture Medium by Response Surface Methodology for Low Cost Production of Antifungal Activity Microorganisms antifungal activity response surface methodology Plackett–Burman design central composite design polynomial and trigonometric regression models |
| title | Optimization of <i>Bacillus amyloliquefaciens</i> BLB369 Culture Medium by Response Surface Methodology for Low Cost Production of Antifungal Activity |
| title_full | Optimization of <i>Bacillus amyloliquefaciens</i> BLB369 Culture Medium by Response Surface Methodology for Low Cost Production of Antifungal Activity |
| title_fullStr | Optimization of <i>Bacillus amyloliquefaciens</i> BLB369 Culture Medium by Response Surface Methodology for Low Cost Production of Antifungal Activity |
| title_full_unstemmed | Optimization of <i>Bacillus amyloliquefaciens</i> BLB369 Culture Medium by Response Surface Methodology for Low Cost Production of Antifungal Activity |
| title_short | Optimization of <i>Bacillus amyloliquefaciens</i> BLB369 Culture Medium by Response Surface Methodology for Low Cost Production of Antifungal Activity |
| title_sort | optimization of i bacillus amyloliquefaciens i blb369 culture medium by response surface methodology for low cost production of antifungal activity |
| topic | antifungal activity response surface methodology Plackett–Burman design central composite design polynomial and trigonometric regression models |
| url | https://www.mdpi.com/2076-2607/10/4/830 |
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