A single mutation in Crimean-Congo hemorrhagic fever virus discovered in ticks impairs infectivity in human cells
Crimean-Congo hemorrhagic fever (CCHF) is the most widely distributed tick-borne viral infection in the world. Strikingly, reported mortality rates for CCHF are extremely variable, ranging from 5% to 80% (Whitehouse, 2004). CCHF virus (CCHFV, Nairoviridae) exhibits extensive genomic sequence diversi...
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2020-10-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/50999 |
| _version_ | 1818028500096909312 |
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| author | Brian L Hua Florine EM Scholte Valerie Ohlendorf Anne Kopp Marco Marklewitz Christian Drosten Stuart T Nichol Christina Spiropoulou Sandra Junglen Éric Bergeron |
| author_facet | Brian L Hua Florine EM Scholte Valerie Ohlendorf Anne Kopp Marco Marklewitz Christian Drosten Stuart T Nichol Christina Spiropoulou Sandra Junglen Éric Bergeron |
| author_sort | Brian L Hua |
| collection | DOAJ |
| description | Crimean-Congo hemorrhagic fever (CCHF) is the most widely distributed tick-borne viral infection in the world. Strikingly, reported mortality rates for CCHF are extremely variable, ranging from 5% to 80% (Whitehouse, 2004). CCHF virus (CCHFV, Nairoviridae) exhibits extensive genomic sequence diversity across strains (Deyde et al., 2006; Sherifi et al., 2014). It is currently unknown if genomic diversity is a factor contributing to variation in its pathogenicity. We obtained complete genome sequences of CCHFV directly from the tick reservoir. These new strains belong to a solitary lineage named Europe 2 that is circumstantially reputed to be less pathogenic than the epidemic strains from Europe 1 lineage. We identified a single tick-specific amino acid variant in the viral glycoprotein region that dramatically reduces its fusion activity in human cells, providing evidence that a glycoprotein precursor variant, present in ticks, has severely impaired function in human cells. |
| first_indexed | 2024-12-10T05:04:47Z |
| format | Article |
| id | doaj.art-272cbf6709bc4baf8f454ca96039430a |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-12-10T05:04:47Z |
| publishDate | 2020-10-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-272cbf6709bc4baf8f454ca96039430a2022-12-22T02:01:16ZengeLife Sciences Publications LtdeLife2050-084X2020-10-01910.7554/eLife.50999A single mutation in Crimean-Congo hemorrhagic fever virus discovered in ticks impairs infectivity in human cellsBrian L Hua0https://orcid.org/0000-0002-7580-3399Florine EM Scholte1https://orcid.org/0000-0003-2110-3087Valerie Ohlendorf2Anne Kopp3Marco Marklewitz4https://orcid.org/0000-0003-1828-8770Christian Drosten5Stuart T Nichol6Christina Spiropoulou7https://orcid.org/0000-0001-8406-3161Sandra Junglen8Éric Bergeron9https://orcid.org/0000-0003-3398-8628Centers for Disease Control and Prevention, Atlanta, United StatesCenters for Disease Control and Prevention, Atlanta, United StatesInstitute of Virology, Charité-Universitätsmedizin Berlin, corporate member of Free University Berlin, Humboldt-University Berlin, and Berlin Institute of Health, Berlin, Germany; German Center for Infection Research (DZIF), Berlin, GermanyInstitute of Virology, Charité-Universitätsmedizin Berlin, corporate member of Free University Berlin, Humboldt-University Berlin, and Berlin Institute of Health, Berlin, Germany; German Center for Infection Research (DZIF), Berlin, GermanyInstitute of Virology, Charité-Universitätsmedizin Berlin, corporate member of Free University Berlin, Humboldt-University Berlin, and Berlin Institute of Health, Berlin, Germany; German Center for Infection Research (DZIF), Berlin, GermanyInstitute of Virology, Charité-Universitätsmedizin Berlin, corporate member of Free University Berlin, Humboldt-University Berlin, and Berlin Institute of Health, Berlin, Germany; German Center for Infection Research (DZIF), Berlin, GermanyCenters for Disease Control and Prevention, Atlanta, United StatesCenters for Disease Control and Prevention, Atlanta, United StatesInstitute of Virology, Charité-Universitätsmedizin Berlin, corporate member of Free University Berlin, Humboldt-University Berlin, and Berlin Institute of Health, Berlin, Germany; German Center for Infection Research (DZIF), Berlin, GermanyCenters for Disease Control and Prevention, Atlanta, United StatesCrimean-Congo hemorrhagic fever (CCHF) is the most widely distributed tick-borne viral infection in the world. Strikingly, reported mortality rates for CCHF are extremely variable, ranging from 5% to 80% (Whitehouse, 2004). CCHF virus (CCHFV, Nairoviridae) exhibits extensive genomic sequence diversity across strains (Deyde et al., 2006; Sherifi et al., 2014). It is currently unknown if genomic diversity is a factor contributing to variation in its pathogenicity. We obtained complete genome sequences of CCHFV directly from the tick reservoir. These new strains belong to a solitary lineage named Europe 2 that is circumstantially reputed to be less pathogenic than the epidemic strains from Europe 1 lineage. We identified a single tick-specific amino acid variant in the viral glycoprotein region that dramatically reduces its fusion activity in human cells, providing evidence that a glycoprotein precursor variant, present in ticks, has severely impaired function in human cells.https://elifesciences.org/articles/50999congo hemorrhagic fever virusnairoviridaetick-borne virusvirus pathogenesismembrane fusionphylogenetic |
| spellingShingle | Brian L Hua Florine EM Scholte Valerie Ohlendorf Anne Kopp Marco Marklewitz Christian Drosten Stuart T Nichol Christina Spiropoulou Sandra Junglen Éric Bergeron A single mutation in Crimean-Congo hemorrhagic fever virus discovered in ticks impairs infectivity in human cells eLife congo hemorrhagic fever virus nairoviridae tick-borne virus virus pathogenesis membrane fusion phylogenetic |
| title | A single mutation in Crimean-Congo hemorrhagic fever virus discovered in ticks impairs infectivity in human cells |
| title_full | A single mutation in Crimean-Congo hemorrhagic fever virus discovered in ticks impairs infectivity in human cells |
| title_fullStr | A single mutation in Crimean-Congo hemorrhagic fever virus discovered in ticks impairs infectivity in human cells |
| title_full_unstemmed | A single mutation in Crimean-Congo hemorrhagic fever virus discovered in ticks impairs infectivity in human cells |
| title_short | A single mutation in Crimean-Congo hemorrhagic fever virus discovered in ticks impairs infectivity in human cells |
| title_sort | single mutation in crimean congo hemorrhagic fever virus discovered in ticks impairs infectivity in human cells |
| topic | congo hemorrhagic fever virus nairoviridae tick-borne virus virus pathogenesis membrane fusion phylogenetic |
| url | https://elifesciences.org/articles/50999 |
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